Publications by authors named "Teresa Duran-Valle"
Article Synopsis
- This study aims to quickly and accurately detect the Y132F ERG11p mutation in Candida parapsilosis isolates and to analyze genetic diversity among these isolates for better tracking of fluconazole resistance.!* -
- The research involved 223 isolates from patients across 20 hospitals in Spain and Italy, using newly optimized PCR methods that require no DNA extraction and provide results in as little as 1.5 hours.!* -
- The findings confirm both PCR methods have 100% specificity and sensitivity, with Scheme 1 showing greater genetic diversity compared to Scheme 2, suggesting the use of specific microsatellite markers for better genotyping of resistant strains.!*
Background: Fluconazole-resistant Candida parapsilosis is a matter of concern.
Objectives: To describe fluconazole-resistant C. parapsilosis genotypes circulating across hospitals in Spain and Rome and to study their azole-resistance profile associated with ERG11p substitutions.
We performed a clinical evaluation of the Vitek MS matrix-assisted laser desorption ionization-time-of-flight mass spectrometry (MALDI-TOF MS) system with the commercial database version 2.0 for rapid identification of medically important yeasts as compared with the conventional phenotypic method API ID 32 C. We tested 161 clinical isolates, nine isolates from culture collections and five reference strains.
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