[Cytokine regulation in patients with brain tumours].

The aim of the study was to investigate serum levels, as well as the ability to produce in vitro, some pro-inflammatory and anti-inflammatory cytokines in patients with brain tumours (BT). Serum concentrations of IFN-gamma, IFN-alpha, TNF-alpha, IL-4, IL-6, IL-8, and RA IL-1 were determined in 17 patients with brain tumours, and in 21 healthy donors. The results showed that baseline levels of cytokines studied was above normal in most patients (78%) had the highest rates of IFN-gamma.

[The influence of solcoseryl on population and cellular parameters of HeLa and RD cultures].

Changes of population and cellular parameters of HeLa and RD cultures after introducing of solcoseryl in culture medium were studied by methods of scanning cytophotometry and cytomorphometry. Monolayer density, proliferation activity, the number of dead cells in a monolayer, the number of nucleoli in nuclei and distribution of cells in the populations by this parameter, RNA and DNA masses in nuclei and nucleoli, total volumes and surface areas of the nuclei and nucleoli were determined. It has been shown that solcoseryl differently affects the cultures both on population and on cellular levels of their organization.

[Immune reactivity and cytokine status in polytrauma].

The PHA-induced and serum levels of IFN-alpha and -gamma, TNF-alpha, IL-4, IL-6, IL-8, IL-RA, as well as CD3, CD4, CD8, CD20, CD56 lymphocytic subpopulations and serum IgG, IgM, and IgA were studied in 53 patients with multiple trauma to detect the most informative parameters of laboratory monitoring of the immunodeficiency developing during treatment. The stages of the study corresponded to the phases of development of an immune response: (1) an induction phase (days 1-4); (2) an immunoregulatory phase (days 5-10); (3) an effector phase (days 11-24). The findings suggest that at the early stage of the study, the drastic reduction was revealed in CD3, CD4 T-lymphocytic subpopulations, accompanied by inadequate humoral immunological parameters.

[Multiparametric study and cluster-analysis of the transformed cultures of human cells].

The cells of nepatocarcinoma (HEp-G2), adenocarcinoma of large intestine (Caco-2), embryonal kidney (HEK-293), neuroblastoma (SH-SY5Y), rabdomyosarcoma (RD), and larynx cancer (Hep-2) were studied by the methods of scanning cytophotometry, cytochemistry and cytomorphometry during 96 h of cultivation. The density of monolayers, proliferation activity, the number of dead cells, DNA content in the nuclei and distribution of the cells in the population by this parameter, total DNA content in the nucleoli (circumnucleolar chromatin), the number of nucleoli in the nuclei, distribution of cells by their number, the volume and area of the nucleus surface, total volume and area of the nucleoli surface were determined. The data obtained were used in the treelike cluster analysis of the cultures by Pierson correlation.

[Human large intestine adenocarcinoma cells (CaCo-2) in the process of cultivation].

Using cytomorphometry and cytophotometry cells of human large intestine adenocarcinoma (CaCo-2) were studied under condition of a 10 day cultivation. A reverse dependence was established between proliferative activity and monolayer density. The increase of the latter inhibits proliferation and promotes the formation of islets of polymorph cells.

[Behavior of HEp-2 cell line cells, their nuclei and nucleoli during cultivation and under the effect of Na-ds-RNA].

The behavior of human larynx cancer cells (HEp-2) and of their nuclei and nucleoli during the cultivation without the influence of Na-ds-RNA and after its introduction into the medium was investigated by methods of cytomorphometry and cytophotometry. The density of monolayer (the number of cells on the area unit), percentage of two-nuclear cells, the number of nucleoli in the nuclei, mitotic coefficient, volume and total surface of nuclei and nucleoli have been measured. In addition, the mass of DNA in the nuclei and that of the total RNA and DNA in the nuclei and in each nucleolus was measured.

[Various immunological and immunomorphological changes in patients with breast cancer after interferon administration].

The immunologic and immunomorphologic effect of native alpha-interferon eta phi-II Parenteral) in 12 patients with stage II breast cancer was studied. A preoperative course of interferon injected into tumor was found to induce local and general immunomodulating effect which suggested activation of cellular antitumor immunity.

[Modulating action of interferon preparations on cellular reactions of antitumor immunity].

The study established an immunomodulating effect of preparations of human leukocytic interferon in spontaneous rosette-formation test (E--RFC) and leukocyte adherence inhibition reaction (LAI) which takes place both in healthy donors and patients with cervical carcinoma. In E--RFC test, administration of interferon preparations caused the E-rosette forming cell fraction to increase in donors. A similar but much lower effect was observed in the patient group.