Effect of porous structure of macroporous polymer supports on resolution in high-performance membrane chromatography of proteins.

The effect of porous structures of 2-mm thick diethylamine functionalized monolithic polymethacrylate discs on their chromatographic behavior in ion-exchange mode has been studied. Discs with small pores did not perform well because they exhibited high back pressure and substantial peak broadening. Discs characterized with pores larger than 1,000 nm did not provide good separations either because the time required for some protein molecules to traverse the length across the pore to reach the wall for adsorption/desorption process that is essential for the separation may be longer than their residence time within the matrix.

[Additive effect of repeated impulse air waves on the activity of rat and human hepatic enzymes].

Impulse air waves (IAW) applied in non-destructive and weakly destructive intensities reduced the activity of lysosomal and microsomal enzymes as well as the activity of adenylate cyclase in the liver of laboratory rats. Five times repeated single application of IAW in intensities causing no noticeable biochemical (and morphological) changes led to shifts in the activity of rat hepatic enzymes. IAW which failed to produce a biological effect in humans when applied separately, inhibited noticeably antipyrine biotransformation in volunteers when applied repeatedly 80 times.