Effects of TRPC1's Lysines on Heteromeric TRPC5-TRPC1 Channel Function.

Background: TRPC5 proteins form plasma membrane cation channels and are expressed in the nervous and cardiovascular systems. TRPC5 activation leads to cell depolarization and increases neuronal excitability, whereas a homologous TRPC1 inhibits TRPC5 function via heteromerization. The mechanism underlying the inhibitory effect of TRPC1 in TRPC5/TRPC1 heteromers remains unknown.

Epipleoneura parmula sp. nov. (Odonata: Coenagrionidae: Protoneurinae) from Amazonian Ecuador.

Epipleoneura parmula sp. nov. is described and illustrated based on males from eastern Ecuador (holotype: , ECUADOR, Orellana Province, Primavera, Laguna Taracoa {0.

A Catalogue and Historical Study of the Odonata of Ecuador.

We present a list of 425 valid Odonata species from Ecuador, providing a brief overview of each genus, a photograph of a representative species of each genus, and all records known to us for each species. Thirty-eight of the 425 species were previously unrecorded from Ecuador. Data were derived from published records, public and private collections, and field records accumulated since the 1960's by the authors and others who have contributed specimen information to this study.

Phylogenetic relationships of North American Gomphidae and their close relatives.

Intrafamilial relationships among clubtail dragonflies (Gomphidae) have been the subject of many morphological studies, but have not yet been systematically evaluated using molecular data. Here we present the first molecular phylogeny of Gomphidae. We include six of the eight subfamilies previously suggested to be valid, and evaluate generic relationships within them.

IMG/M: integrated genome and metagenome comparative data analysis system.

The Integrated Microbial Genomes with Microbiome Samples (IMG/M: https://img.jgi.doe.

Psaironeura angeloi, a new species of damselfly (Zygoptera: Coenagrionidae) from Central and South America.

Psaironeura angeloi sp. nov. (Holotype male deposited in FSCA: ECUADOR, Esmeraldas Province, small stream 5.

The standard operating procedure of the DOE-JGI Metagenome Annotation Pipeline (MAP v.4).

The DOE-JGI Metagenome Annotation Pipeline (MAP v.4) performs structural and functional annotation for metagenomic sequences that are submitted to the Integrated Microbial Genomes with Microbiomes (IMG/M) system for comparative analysis. The pipeline runs on nucleotide sequences provided via the IMG submission site.

Discovery of Novel Plant Interaction Determinants from the Genomes of 163 Root Nodule Bacteria.

Root nodule bacteria (RNB) or "rhizobia" are a type of plant growth promoting bacteria, typified by their ability to fix nitrogen for their plant host, fixing nearly 65% of the nitrogen currently utilized in sustainable agricultural production of legume crops and pastures. In this study, we sequenced the genomes of 110 RNB from diverse hosts and biogeographical regions, and undertook a global exploration of all available RNB genera with the aim of identifying novel genetic determinants of symbiotic association and plant growth promotion. Specifically, we performed a subtractive comparative analysis with non-RNB genomes, employed relevant transcriptomic data, and leveraged phylogenetic distribution patterns and sequence signatures based on known precepts of symbiotic- and host-microbe interactions.

ProDeGe: a computational protocol for fully automated decontamination of genomes.

Single amplified genomes and genomes assembled from metagenomes have enabled the exploration of uncultured microorganisms at an unprecedented scale. However, both these types of products are plagued by contamination. Since these genomes are now being generated in a high-throughput manner and sequences from them are propagating into public databases to drive novel scientific discoveries, rigorous quality controls and decontamination protocols are urgently needed.

IMG/M 4 version of the integrated metagenome comparative analysis system.

IMG/M (http://img.jgi.doe.

Clusters and superclusters of phased small RNAs in the developing inflorescence of rice.

To address the role of small regulatory RNAs in rice development, we generated a large data set of small RNAs from mature leaves and developing roots, shoots, and inflorescences. Using a spatial clustering algorithm, we identified 36,780 genomic groups of small RNAs. Most consisted of 24-nt RNAs that are expressed in all four tissues and enriched in repeat regions of the genome; 1029 clusters were composed primarily of 21-nt small RNAs and, strikingly, 831 of these contained phased RNAs and were preferentially expressed in developing inflorescences.

Mosquito and arbovirus activity during 1997-2002 in a wetland in northeastern Mississippi.

The species composition and population dynamics of adult mosquitoes in a wetland near Iuka, MS, were analyzed over a 6-yr period (1997-2002) and reverse transcription-polymerase chain reaction (PCR) detection rates of arboviruses determined during five of those years. Blood meals of three likely vector species were identified using a PCR-based method that allows identification of the host to species. Culex erraticus (Dyar & Knab) composed 51.

Simultaneous detection of three mosquito-borne encephalitis viruses (eastern equine, La Crosse, and St. Louis) with a single-tube multiplex reverse transcriptase polymerase chain reaction assay.

Three mosquito-borne human encephalitis viruses (eastern equine encephalitis virus [EEE], St. Louis encephalitis virus [SLE], and La Crosse encephalitis virus [LAC]) are sympatric in the southeastern United States. However, little is known concerning the temporal and spatial pattern of the distribution of these viruses in this area.