[Comparative study on nutritional status of patients in intensive care unit with different assessment tools].

Objective: To evaluate the nutritional status of patients in intensive care unit (ICU) by using nutritional risk screening 2002 scale (NRS2002), subjective general assessment (SGA) and critical illness nutritional risk score (NUTRIC), and to compare the characteristics and applicability of three scoring tools.

Methods: A cross-sectional survey was conducted. 315 patients admitted to the comprehensive ICU of Affiliated Qingdao Municipal Hospital of Qingdao University from April 2018 to July 2019 were enrolled.

[Preliminary study on the effect of lipopolysaccharide challenge on the genes expression of inflammatory factors in A549 cells].

Objective: To explore the effects of lipopolysaccharide (LPS) on the expression of inflammatory genes in A549 cells line under different concentrations and different action time, this study laid the foundation for further establishment of acute respiratory distress syndrome (ARDS) cell model in the optimal concentration-time way.

Methods: A549 cells line was incubated routinely in 5% CO incubator at 37 centigrade with high glucose DMEM medium which included 10% fetal calf serum. Cells in logarithmic phase was cultured for passage, the cells was count to adjust cell density to (5-7)×10 and tile evenly in six-hole plate.