Study on Mechanism of Mechanical Property Enhancement of Expansive Soil by Alkali-Activated Slag.

Expansive soil poses significant challenges for engineering due to its susceptibility to swelling and shrinkage. This study aims to explore effective methods for improving its mechanical properties using single alkaline activators, single slag, and their combination. Laboratory experiments were conducted to evaluate the unconfined compressive strength (UCS) and analyze curing mechanisms through X-ray diffraction (XRD) and scanning electron microscopy (SEM).

Transcriptome Regulation Mechanisms Difference between Female and Male in Response to Drought Stress and Rehydration.

Drought, a pervasive global challenge, significantly hampers plant growth and crop yields, with drought stress being a primary inhibitor. Among resilient species, , a warm-season and dioecious turfgrass, stands out for its strong drought resistance and minimal maintenance requirements, making it a favored choice in ecological management and landscaping. However, there is limited research on the physiological and molecular differences in drought resistance between male and female To decipher the transcriptional regulation dynamics of these sexes in response to drought, RNA-sequencing analysis was conducted using the 'Texoka' cultivar as a model.

Transcriptomic and physiological analysis provide new insight into seed shattering mechanism in Pennisetum alopecuroides 'Liqiu'.

Through the histological, physiological, and transcriptome-level identification of the abscission zone of Pennisetum alopecuroides 'Liqiu', we explored the structure and the genes related to seed shattering, ultimately revealing the regulatory network of seed shattering in P. alopecuroides. Pennisetum alopecuroides is one of the most representative ornamental grass species of Pennisetum genus.

Octoploids Show Enhanced Salt Tolerance through Chromosome Doubling in Switchgrass ( L.).

Polyploid plants often exhibit enhanced stress tolerance. Switchgrass is a perennial rhizomatous bunchgrass that is considered ideal for cultivation in marginal lands, including sites with saline soil. In this study, we investigated the physiological responses and transcriptome changes in the octoploid and tetraploid of switchgrass ( L.

Chromosome-level reference genome assembly provides insights into the evolution of .

is an important forage grass resource, which plays a vital role in ecological environment improvement. Therefore, the acquisition of genome resources is conducive to the study of the adaptability of species in ecological remediation and forage breeding development. Here we assembled a cv.

The first complete mitochondrial genome of Carex (C. breviculmis): a significantly expanded genome with highly structural variations.

The first complete mitochondrial genome of Carex (C. breviculmis) was sequenced and assembled, and its genomic signature was analyzed and the possible conformations of its mitochondrial genome were validated. Carex breviculmis is a very adaptable grass that is highly resistant to environmental stresses such as drought and low light.

Enhanced methane production in microbial electrolysis cell coupled anaerobic digestion system with MXene accelerants.

Accelerants can improve the anaerobic performance of a microbial electrolysis cell coupled anaerobic digestion (MEC-AD). MAX phase titanium aluminum carbide (MAX), multilayer TiCT MXene (ML-MXene) and few-layer TiCT MXene (FL-MXene) were utilized as accelerants for MEC-AD to promote CH production and CO reduction at a voltage of 0.6 V.

Chloroplast genomes of four species: Long repetitive sequences trigger dramatic changes in chloroplast genome structure.

The chloroplast genomes of angiosperms usually have a stable circular quadripartite structure that exhibits high consistency in genome size and gene order. As one of the most diverse genera of angiosperms, is of great value for the study of evolutionary relationships and speciation within its genus, but the study of the structure of its chloroplast genome is limited due to its highly expanded and restructured genome with a large number of repeats. In this study, we provided a more detailed account of the chloroplast genomes of using a hybrid assembly of second- and third-generation sequencing and examined structural variation within this genus.

Dual utilization of aloe peel: Aloe peel-derived carbon quantum dots enhanced anaerobic co-digestion of aloe peel.

Carbon materials have been widely used in anaerobic digestion (AD), but the role of zero-dimensional carbon quantum dots (CQDs) in anaerobic co-digestion (AcoD) has not yet been reported. In this work, the effect of aloe peel-derived CQDs (AP-CQDs) on the AcoD system of aloe peel and dairy manure was investigated. The addition of AP-CQDs accelerants increased the cumulative CH yield from 201.

Enhanced methane production in anaerobic co-digestion systems with modified black phosphorus.

Black phosphorus (BP) and BP modified by hydrogen peroxide (MBP) were used as accelerants to enhance CH production and CO reduction in microbial electrolysis cells (MECs) coupled with anaerobic co-digestion systems (MEC-AcoD). The MEC-AcoD group with a voltage of 0.6 V and 0.

Overexpression of from , which could interact with ABA2, improved plant cold tolerance mediated by ABA signaling.

ABI4 is considered an important transcription factor with multiple regulatory functions involved in many biological events. However, its role in abiotic stresses, especially low-temperature-induced stress, is poorly understood. In this study, the gene was derived from , a widely used forage grass.

Effects of Nitrogen Forms on the Growth and Nitrogen Accumulation in Seedlings.

Buffalograss [ (Nutt.) Engelm.] has become the most widely cultivated warm-season turfgrass in northern China because of its low-maintenance requirements.

Single-molecule long-read sequencing analysis improves genome annotation and sheds new light on the transcripts and splice isoforms of Zoysia japonica.

Background: Zoysia japonica is an important warm-season turfgrass used worldwide. Although the draft genome sequence and a vast amount of next-generation sequencing data have been published, the current genome annotation and complete mRNA structure remain incomplete. Therefore, to analyze the full-length transcriptome of Z.

Chlorophyll Reductase Gene Participates in Chlorophyll Degradation and Photosynthesis.

The degradation of chlorophyll is of great significance to plant growth. The chlorophyll reductase NOL (NYC1-like) is in charge of catalyzing the degradation of chlorophyll and maintaining the stability of the photosystem. However, the molecular mechanisms of NOL-mediated chlorophyll degradation, senescence, and photosynthesis and its functions in other metabolic pathways remain unclear, especially in warm-season turfgrass.

Combination analysis of single-molecule long-read and Illumina sequencing provides insights into the anthocyanin accumulation mechanism in an ornamental grass, Pennisetum setaceum cv. Rubrum.

Combination analysis of single-molecule long-read and Illumina sequencing provide full-length transcriptome information and shed new light on the anthocyanin accumulation mechanism of Pennisetum setaceum cv. 'Rubrum'. Pennisetum setaceum cv.

Seed Germination Mechanism of Under Variable Temperature Determinded Using Integrated Single-Molecule Long-Read and Illumina Sequence Analysis.

The turfgrass species has broad development and utilization prospects in landscaping construction. However, seed dormancy and a low germination rate have inhibited its application. Furthermore, the molecular mechanisms of seed germination in have not been thoroughly studied.

Transcriptome profiling revealed candidate genes, pathways and transcription factors related to nitrogen utilization and excessive nitrogen stress in perennial ryegrass.

Ryegrass (Lolium perenne L.), a high-quality forage grass, is a good nutrient source for herbivorous livestock. However, improving nitrogen use efficiency and avoiding nitrate toxicity caused by excessive nitrogen are continual challenges in ryegrass production.

Expression of , a Phytoene Synthase Gene from Affects Plant Height and Photosynthetic Pigment Contents.

Phytoene synthase (PSY) is a key limiting enzyme in the carotenoid biosynthesis pathway for regulating phytoene synthesis. In this study, was isolated and identified from , an important lawn grass species. cDNA was 1230 bp in length, corresponding to 409 amino acids.

Use of bag-filter gas dust in anaerobic digestion of cattle manure for boosting the methane yield and digestate utilization.

Plenty of refractory and environmentally hazardous bag-filter gas dust (BGD) is produced in the iron-making process. The effects of untreated BGD on anaerobic digestion (AD) with cattle manure were investigated. The BGD had the potential to boost the methane yield and digestate utilization considerably.

Functional Characterization of the Pheophytinase Gene, , From in Regulating Chlorophyll Degradation and Photosynthesis.

Pheophytinase (PPH), the phytol hydrolase, plays important roles in chlorophyll degradation. Nevertheless, little attention has been paid to the PPHs in warm-season grass species; neither its detailed function in photosynthesis has been systematically explored to date. In this study, we isolated from , an excellent warm-season turfgrass species.

Enhanced anaerobic co-digestion performance by using surface-annealed titanium spheres at different atmospheres.

A series of surface-annealed titanium spheres (Ti-A, Ti-B, and Ti-C) in different atmospheres were used as accelerants in anaerobic co-digestion (AcoD) systems under magnetic field (MF). Surface-annealed titanium spheres and MF exhibit remarkable coupling and promoting effects on the AcoD performance. The cumulative biogas yield (435.

Coconut-shell-derived bio-based carbon enhanced microbial electrolysis cells for upgrading anaerobic co-digestion of cow manure and aloe peel waste.

Microbial electrolysis cells (MECs) and exogenous accelerants can augment anaerobic digestion performance. Herein, MECs and coconut-shell-derived bio-based carbon (CBC) accelerant are adopted to strengthen anaerobic co-digestion of cow manure and aloe peel waste. The MEC with the voltage of 0.

The development of SSR markers based on RNA-sequencing and its validation between and within Carex L. species.

Background: Carex L. is one of the largest genera in the Cyperaceae family and an important vascular plant in the ecosystem. However, the genetic background of Carex is complex and the classification is not clear.

PacBio single-molecule long-read sequencing shed new light on the transcripts and splice isoforms of the perennial ryegrass.

Perennial ryegrass (Lolium perenne), one of the most widely used forage and cool-season turfgrass worldwide, has a breeding history of more than 100 years. However, the current draft genome annotation and transcriptome characterization are incomplete mainly because of the enormous difficulty in obtaining full-length transcripts. To explore the complete structure of the mRNA and improve the current draft genome, we performed PacBio single-molecule long-read sequencing for full-length transcriptome sequencing in perennial ryegrass.

PacBio single-molecule long-read sequencing shed new light on the complexity of the Carex breviculmis transcriptome.

Background: Carex L., a grass genus commonly known as sedges, is distributed worldwide and contributes constructively to turf management, forage production, and ecological conservation. The development of next-generation sequencing (NGS) technologies has considerably improved our understanding of transcriptome complexity of Carex L.