Novel molecular diagnostic (PCR) diagnosis and outcome of intestinal Echinococcus multilocularis in a dog from western Canada.

Objective: To describe the novel PCR diagnosis and outcome of intestinal Echinococcus multilocularis in a dog.

Animal: A 13-month-old female intact dog with naturally occurring intestinal E multilocularis.

Clinical Presentation, Progression, And Procedures: The 13-month-old dog initially presented with a reduced appetite and weight loss and then developed hematochezia.

New geographic records for in coyotes and moose from Nova Scotia, Canada.

spp. tapeworms can cause serious diseases in mammals, including humans. Within the species complex, metacestodes produce unilocular cysts that are responsible for cystic echinococcosis in animal intermediate hosts.

Host and geographic differences in prevalence and diversity of gastrointestinal helminths of foxes (), coyotes () and wolves () in Québec, Canada.

Wild canids are hosts to a wide range of parasites and can play a role in transmission of zoonoses. As many parasites are transmitted through food webs, and wild canids are at high trophic levels, parasite prevalence and diversity in wild canids can serve as excellent indicators of ecosystem health. Our main objectives were to update knowledge on the composition of gastrointestinal helminths in wild canids from Québec, Canada, and to describe differences in parasite prevalence and diversity among canid species and regions.

Copro-polymerase chain reaction has higher sensitivity compared to centrifugal fecal flotation in the diagnosis of taeniid cestodes, especially Echinococcus spp, in canids.

Prompt and reliable diagnostic tests for taeniid infection in canids are important due to the risk of zoonoses like Echinococcus spp. Current diagnostic methods relying on fecal flotation lack sensitivity and specificity, but this has rarely been quantified due to the challenges in performing adult cestode recovery (the gold standard) in domestic dogs (Canis familiaris). Therefore, we recovered adult Taenia and Echinococcus spp.

Molecular Evidence for Local Acquisition of Human Alveolar Echinococcosis in Saskatchewan, Canada.

Alveolar echinococcosis (AE) is a life-threatening parasitic disease caused by the zoonotic cestode Echinococcus multilocularis. Our goals were to confirm infection, identify species, and analyze biogeographical origin of metacestode tissues from a suspected human AE case in Saskatchewan, Canada. We conducted polymerase chain reaction (PCR) targeting the nad1 mitochondrial gene for E.

COVID-19: CADD to the rescue.

The recent outbreak of the deadly COVID-19 disease, being caused by the novel coronavirus (SARS-CoV-2), has put the world on red alert as it keeps spreading and recording more fatalities. Research efforts are being carried out to curtail the disease from spreading as it has been declared as of global health emergency. Hence, there is an exigent need to identify and design drugs that are capable of curing the infection and hinder its continual spread across the globe.

Molecular dynamics and combined docking studies for the identification of Zaire ebola virus inhibitors.

Ebola virus (EBOV) is a lethal human pathogen with a risk of global spread of its zoonotic infections, and specifically has the highest fatality rate amongst other species. There is a need for continuous effort towards having therapies, as a single licensed treatment to neutralize the EBOV is yet to come into reality. This present study virtually screened the MCULE database containing almost 36 million compounds against the structure of a Zaire Ebola viral protein (VP) 35 and a consensus scoring of both MCULE and CLCDDW docking programs remarked five compounds as potential hits.

Structure based virtual screening of the Ebola virus trimeric glycoprotein using consensus scoring.

Ebola virus (EBOV) causes zoonotic viral infection with a potential risk of global spread and a highly fatal effect on humans. Till date, no drug has gotten market approval for the treatment of Ebola virus disease (EVD), and this perhaps allows the use of both experimental and computational approaches in the antiviral drug discovery process. The main target of potential vaccines that are recently undergoing clinical trials is trimeric glycoprotein (GP) of the EBOV and its exact crystal structure was used in this structure based virtual screening study, with the aid of consensus scoring to select three possible hit compounds from about 36 million compounds in MCULE's database.

Identification of potential inhibitors against the Zika virus using consensus scoring.

The Zika virus (ZIKV) is a life threatening pathogen of zoonotic importance with prevalence in some parts of Africa and America. Unfortunately, there is yet to be a single approved vaccine or antiviral drug to treat the diseases and deformations being caused by the Zika virus infection. In this study, about 36 million compounds from MCULE database were virtually screened against a real matured ZIKV protein using a consensus scoring method to get improved hit rates.

Seroprevalence of canine leishmaniasis in Kwara, Oyo and Ogun states of Nigeria.

Leishmaniasis is an important tropical disease that is gradually gaining attention in Nigeria. The canine species which include domestic dogs have been named the reservoir host for the zoonotic form of leishmaniasis. The present study was carried out to determine the seroprevalence of canine leishmaniasis in three selected states of Nigeria using indirect enzyme linked immunosorbent assay (ELISA).

Echinococcus granulosus Prevalence in Dogs in Southwest Nigeria.

Echinococcosis is a public health parasitic disease that is cosmopolitan (Echinococcus granulosus) in its distribution. Domestic dogs (Canis familiaris) have been recognised as the definitive host of the parasite. The present study was carried out to determine the prevalence of canine echinococcosis in Southwest Nigeria using direct enzyme linked immunosorbent assay (ELISA) to detect sera antigen.