Background: Gamma-polyglutamic acid (γ-PGA) is a microbially produced non-toxic peptide biopolymer which is gaining grounds in many biotechnological fields and has a wide range of applications.
Objectives: In this study, the characteristics of γ-PGA produced by Bacillus megaterium isolated from an oil seed were determined, while the nutritional requirements of the bacterium were optimized using a predictive 15 factor-16 run Plackett-Burman experimental design.
Material And Methods: The main effect of each factor, the interaction and quadratic effects of the factors on optimized production were determined from Box-Benkhen model using Dell Statistica v.