Biobased monoliths for adenovirus purification.

Adenoviruses are important platforms for vaccine development and vectors for gene therapy, increasing the demand for high titers of purified viral preparations. Monoliths are macroporous supports regarded as ideal for the purification of macromolecular complexes, including viral particles. Although common monoliths are based on synthetic polymers as methacrylates, we explored the potential of biopolymers processed by clean technologies to produce monoliths for adenovirus purification.

Structural evaluation of an alternative Protein A biomimetic ligand for antibody purification.

Affinity chromatography is one of the most common techniques employed at the industrial-scale for antibody purification. In particular, the purification of human immunoglobulin G (hIgG) has gained relevance with the immobilization of its natural binding counterpart-Staphylococcus aureus Protein A (SpA) or with the recent development of biomimetic affinity ligands, namely triazine-based ligands. These ligands have been developed in order to overcome economic and leaching issues associated to SpA.

A green approach toward antibody purification: a sustainable biomimetic ligand for direct immobilization on (bio)polymeric supports.

This paper presents a sustainable strategy for improving the capture of antibodies by affinity chromatography. A novel biomimetic ligand (4-((4-chloro-6-(3-hydroxyphenoxy)-1,3,5-triazin-2-yl)oxy)naphthalen-1-ol) (TPN-BM) was synthesized using a greener and simple protocol to overcome solubility limitations associated with ligand 22/8, known as artificial protein A. Furthermore, its subsequent immobilization on chitosan-based monoliths induced by plasma surface activation allowed the design of a fast and efficient chromatographic platform for immunoglobulin G (IgG) purification.

Functional monolithic platforms: chromatographic tools for antibody purification.

Polymer monoliths are an efficient platform for antibody purification. The use of monoclonal antibodies (mAbs) and engineered antibody structures as therapeutics has increased exponentially over the past few decades. Several approaches use polymer monoliths to purify large quantities of antibody with defined clinical and performance requirements.

Anti-biofouling 3D porous systems: the blend effect of oxazoline-based oligomers on chitosan scaffolds.

The production, characterization and anti-biofouling activity of 3D porous scaffolds combining different blends of chitosan and oxazoline-based antimicrobial oligomers is reported. The incorporation of ammonium quaternized oligo(2-oxazoline)s into the composition of the scaffold enhances the stability of the chitosan scaffold under physiological conditions as well as its ability to repel protein adsorption. The blended scaffolds showed mean pore sizes in the range of 18-32 μm, a good pore interconnectivity and high porosity, as well as a large surface area, ultimate key features for anti-biofouling applications.

A Combined Strategy to Surface-Graft Stimuli-Responsive Hydrogels Using Plasma Activation and Supercritical Carbon Dioxide.

Differently shaped polymeric matrices were efficiently coated with stimuli-responsive hydrogels for a wide range of applications using a new methodology. By combining plasma surface activation and polymerization in supercritical media at mild conditions, we report the direct smart coating of microcarriers and membranes in gram-scale quantities with a scalable, green, and low-cost approach.