Publications by authors named "Teerasak E-Kobon"

Article Synopsis
  • Amphotericin B (AmB) is a strong antifungal and antiparasitic drug that kills leishmanial parasites by disrupting their cell membranes, making it important to study its effects on parasites like Leishmania orientalis to understand potential drug resistance.
  • This study involved exposing a specific Leishmania orientalis strain to AmB and analyzing genetic changes through sequencing methods, focusing on single nucleotide polymorphisms (SNPs) and gene expression changes.
  • The results indicated no significant chromosomal alterations post-treatment, but a higher incidence of SNPs in the control group, along with the downregulation of key genes, suggesting complex regulatory mechanisms might contribute to AmB resistance in this strain.
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Certain factors hinder the commercialization of biodesulfurization process, including low substrate-specificity of the currently reported desulfurizing bacteria and restricted mass transfer of organic-sulfur compounds in biphasic systems. These obstacles must be addressed to clean organic-sulfur rich petro-fuels that pose serious environmental and health challenges. In current study, a dibenzothiophene desulfurizing strain, Gordonia rubripertincta W3S5 (source: oil contaminated soil) was systematically evaluated for its potential to remove sulfur from individual compounds and mixture of organic-sulfur compounds.

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Article Synopsis
  • Mitochondrial DNAs (mtDNAs) are important for studying evolutionary relationships and species identification, particularly in eukaryotes, while kinetoplast DNAs (kDNAs) in kinetoplastids have a complex structure, comprising maxicircles and minicircles.
  • This study focused on the kDNAs of a newly examined species, Leishmania orientalis strain PCM2, utilizing hybrid genome sequencing to analyze and reconstruct its kDNA sequences.
  • The research discovered high similarity between L. orientalis maxicircle and another strain, L. enriettii, and identified multiple classes of minicircles, providing genetic insights that may assist in diagnosis and understanding the parasite's genetics in Thailand.
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Dietary fat can alter host metabolism and gut microbial composition. Crocodile oil (CO) was extracted from the fatty tissues of Crocodylus siamensis. CO, rich in monounsaturated- and polyunsaturated fatty acids, has been reported to reduce inflammation, counter toxification, and improve energy metabolism.

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The giant African snail (Order Stylommatophora: Family Achatinidae), (Bowdich, 1822), is the most significant and invasive land snail pest. The ecological adaptability of this snail involves high growth rate, reproductive capacity, and shell and mucus production, driven by several biochemical processes and metabolism. The available genomic information for provides excellent opportunities to hinder the underlying processes of adaptation, mainly carbohydrate and glycan metabolic pathways toward the shell and mucus formation.

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Hyperpigmentation is a medical and cosmetic problem caused by an excess accumulation of melanin or the overexpression of the enzyme tyrosinase, leading to several skin disorders, i.e., freckles, melasma, and skin cancer.

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Article Synopsis
  • - This study focuses on the mitochondrial DNA, or kinetoplast DNA (kDNA), of Leishmania martiniquensis, a species prevalent in Thailand, which is linked to drug resistance and survival in these parasites.
  • - Researchers extracted and reconstructed the complete maxicircle (19,008 bp) and identified 214 classes of minicircles from the L. martiniquensis strain PCM3 using advanced whole-genome sequencing techniques.
  • - The findings reveal close structural similarities between the maxicircle of L. martiniquensis and other Leishmania species, while phylogenetic analysis shows distinct evolution among the minicircles, paving the way for improved diagnostic and monitoring tools for this parasite.
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Background: Pasteurella multocida is an opportunistic pathogen causing porcine respiratory diseases by co-infections with other bacterial and viral pathogens. Various bacterial genera isolated from porcine respiratory tracts were shown to inhibit the growth of the porcine isolates of P. multocida.

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Leishmaniasis is a parasitic disease caused by protozoan flagellates of the genus . Recently, and , emerging species of , were isolated from patients in Thailand. Development of the vaccine is demanded; however, genetic differences between the two species make it difficult to design a vaccine that is effective for both species.

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Article Synopsis
  • The study focuses on the genomic analysis of a neglected strain in Thailand, presenting a new bioinformatic workflow that combines de novo assembly and reference-based methods to generate high-quality genomic drafts from Illumina sequencing data.
  • Using this integrated approach, researchers produced a genomic draft of isolate PCM2, revealing important characteristics such as 3367 contigs and 8887 predicted genes, along with significant integrity and coverage compared to an existing reference genome.
  • The findings suggest that the proposed workflow is effective in generating a valuable genome for further analysis, which could aid in identifying strain-specific markers and virulence genes for drug and vaccine development.
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Article Synopsis
  • Autochthonous leishmaniasis, caused by protozoan parasites, is reported in both immunocompromised and immunocompetent patients in southern Thailand, yet knowledge on its genetics is limited, especially compared to northern isolates.
  • Researchers sequenced and analyzed the genomes of two southern isolates (PCM2 and PCM3) alongside other species, finding notable differences in genome size and protein variations that suggest distinct strains.
  • The study highlights six protein groups linked to virulence and drug resistance, emphasizing the importance of ongoing genetic monitoring of these parasites in Thailand and surrounding areas.
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Acne vulgaris is a common skin disease mainly caused by the Gram-positive pathogenic bacterium, . This bacterium stimulates the inflammation process in human sebaceous glands. The giant African snail () is an alien species that rapidly reproduces and seriously damages agricultural products in Thailand.

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Tropomyosin is a major allergen responsible for cross-allergenicity in a number of shellfish species. Although extensively characterized in marine crustaceans, the information of tropomyosin is limited to a few freshwater crustacean species. As a result, more cross-reactivity evidence and information of tropomyosin at the molecular level are required for the detection of freshwater crustaceans in the food industry.

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produces a capsule composed of different polysaccharides according to the capsular serotype (A, B, D, E, and F). Hyaluronic acid (HA) is a component of certain capsular types of this bacterium, especially capsular type A. Previously, 2 HA biosynthetic genes from a capsular type A strain were studied for the industrial-scale improvement of HA production.

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Skin pigment disorders are common cosmetic and medical problems. Many known compounds inhibit the key melanin-producing enzyme, tyrosinase, but their use is limited due to side effects. Natural-derived peptides also display tyrosinase inhibition.

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Gastropods are among the most diverse animals. Gastropod mucus contains several glycoproteins and peptides that vary by species and habitat. Some bioactive peptides from gastropod mucus were identified only in a few species.

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Background: The porcine respiratory tract harbours multiple microorganisms, and the interactions between these organisms could be associated with animal health status. Pasteurella multocida is a culturable facultative anaerobic bacterium isolated from healthy and diseased porcine respiratory tracts. The interaction between P.

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This study aimed to investigate the conserved FAD-binding region of the L-amino acid oxidase () genes in twelve gastropod genera commonly found in Thailand compared to those in other organisms using molecular cloning, nucleotide sequencing and bioinformatics analysis. Genomic DNA of gastropods and other invertebrates was extracted and screened using primers specific to the conserved FAD-binding region of . The amplified 143-bp fragments were cloned and sequenced.

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Pasteurella multocida causes respiratory infectious diseases in a multitude of birds and mammals. A number of virulence-associated genes were reported across different strains of P. multocida, including those involved in the iron transport and metabolism.

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Background: Pasteurella multocida is a Gram-negative bacterium that causes economically significant infections of a broad range of animal species. Pneumonic and septicaemic pasteurellosis caused by this bacterium remain important problems in pigs, cattle, and water buffaloes in Thailand. The aim of this study was to characterise the virulence-associated gene profiles and to develop an OmpA molecular typing scheme for classifying 191 bovine and porcine isolates of P.

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Several reports have shown antimicrobial and anticancer activities of mucous glycoproteins extracted from the giant African snail Achatina fulica. Anticancer properties of the snail mucous peptides remain incompletely revealed. The aim of this study was to predict anticancer peptides from A.

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