Publications by authors named "Teddy Nakayiki"

Article Synopsis
  • Researchers investigated whether bats in Uganda could be reservoirs for the o'nyong-nyong virus (ONNV) and chikungunya virus (CHIKV) through blood samples.
  • They tested 652 serum samples from six bat species and found that 13.2% of Egyptian rousettes and 8% of little free-tailed bats had antibodies to ONNV.
  • Although interepidemic circulation of ONNV and CHIKV was suggested, the inconsistent presence of antibodies indicates that Egyptian rousette bats are unlikely to be the main reservoirs for these viruses.
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After confirmation of two human cases of Rift Valley fever (RVF) in March 2016 in the Kabale district of Uganda, an entomological investigation was conducted with a focus on mosquito species composition and abundance of known and potential mosquito vector species, and virus testing to identify species most likely involved in Rift Valley fever virus transmission. This information could be used to forecast risk and facilitate improvement of prevention and response tools for use in preventing or controlling future outbreaks. From these collections, two virus isolates were obtained, one each from a pool of Aedes tricholabis and Ae.

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Background: Crimean-Congo Haemorrhagic Fever (CCHF) is an emerging human-health threat causing sporadic outbreaks in livestock farming communities. However, the full extent and the risks associated with exposure of such communities has not previously been well-described.

Methods: We collected blood samples from 800 humans, 666 cattle, 549 goats and 32 dogs in districts within and outside Ugandan cattle corridor in a cross-sectional survey, and tested for CCHFV-specific IgG antibodies using Enzyme-Linked Immunosorbent Assays.

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Article Synopsis
  • Serological cross-reactivity among flaviviruses makes it difficult to determine prior infections in animals in areas with multiple circulating strains.
  • The study hypothesized that subgenomic flavivirus RNA (sfRNA) could indicate Zika virus (ZIKV) infection because it persists in tissues, confirmed by detecting sfRNA in Jamaican fruit bats while NS5 was not present.
  • In a broader application with 198 bat samples from Uganda, sfRNA was detected in four bats from three species, marking the first discovery of ZIKV RNA in East African bats and supporting the use of sfRNA for identifying past flavivirus infections.
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In Uganda, the role of ticks in zoonotic disease transmission is not well described, partly, due to limited available information on tick diversity. This study aimed to identify the tick species that infest cattle. Between September and November 2017, ticks (n = 4362) were collected from 5 districts across Uganda (Kasese, Hoima, Gulu, Soroti, and Moroto) and identified morphologically at Uganda Virus Research Institute.

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While serological and virological evidence documents the exposure of bats to medically-important arboviruses, their role as reservoirs or amplifying hosts is less well-characterized. We describe a novel orbivirus () isolated from an Egyptian fruit bat () trapped in 2013 in Uganda and named Bukakata orbivirus. This is the fifth orbivirus isolated from a bat, however genetic information had previously only been available for one bat-associated orbivirus.

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Morphological abnormalities in ticks seem to be rare phenomena in nature, and are underreported in Africa. In this article, we describe general and local anomalies in two Amblyomma lepidum females and one Rhipicephalus decoloratus female collected from cattle in Moroto and Kasese districts, Uganda. One A.

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