Role of Intracellular Adhesion icaAD and agr genes in Biofilm Formation in Clinical S. aureus Isolates and Assessment of Two Phenotypic Methods.

Objective: To determine the role of and genes in biofilm formation and evaluate the consistency of two phenotypic methods for biofilm measurement.

Methods: A total of 81 clinical strains were included and analyzed for biofilm formation by two methods. The microtitration plate method was optimized using computational fluid dynamics and compared with the Congo red assay.