Publications by authors named "Taylor Martin"

Objective: Female mice exhibit progressive progesterone (P4) deficiency, luteal cell degeneration, and premature embryo implantation failure at 5 months old. We attempted to rescue embryo implantation in non-virgin mice (5-6 months old) with exogenous P4 treatment on days 1.5 post-coitum (D1.

View Article and Find Full Text PDF

Mineral scale formation reduces the heat transfer efficiency and clogs pipes and valves, increasing power consumption. To address the environmental concerns of conventional scale inhibitors, this paper explores biodegradable and eco-friendly alternatives. It examines the effects of organic additives on calcium (Ca) and magnesium (Mg) scaling in water vaporization.

View Article and Find Full Text PDF

Objective: To assist residents in selecting the correct Current Procedural Terminology (CPT) code for evaluation and management (E/M) services thru the addition of disappearing help text into a standardized note template.

Methods: We created a disappearing text block that summarizes E/M requirements and embedded it into the note template used by residents at a pediatric urgent care clinic. An intervention cohort composed of post graduate year 1 (PGY 1) residents was instructed to use this note template, while senior residents (PGY 2-3) were instructed to use an identical template that lacked the help text.

View Article and Find Full Text PDF

Infections with the parasitic protozoan cause Chagas disease, which results in serious cardiac and/or digestive pathology in 30%-40% of individuals. However, symptomatic disease can take decades to become apparent, and there is a broad spectrum of possible outcomes. The complex and long-term nature of this infection places a major constraint on the scope for experimental studies in humans.

View Article and Find Full Text PDF

Background: The International Classification of Headache Disorders (ICHD-3) uses moderate or severe pain intensity in the diagnostic criterion for migraine. However, few studies have analyzed pain rating on a visual analog scale to identify the numerical intensity that correlates with migraine.

Objective: To evaluate the impact of daily self-rated headache pain among patients with either episodic or chronic migraine.

View Article and Find Full Text PDF
Article Synopsis
  • Data-Independent Acquisition (DIA) enhances co-immunoprecipitation analysis by reducing quantitation variability and improving the detection of specific interactors compared to Data-Dependent Acquisition (DDA).
  • A comparison of DIA and DDA across various bioinformatics workflows revealed that DIA can effectively generate spectral libraries without needing separate DDA experiments, and software struggles with indistinct signals from mock pull-downs.
  • Spectronaut and DIA-NN provided the best control of coefficient of variation in protein quantification, while using DIA for both building spectral libraries and quantifying proteins leads to more consistent results and fewer missing values.
View Article and Find Full Text PDF

As the world faces the brink of climatological disaster, it is crucial to utilize all available resources to facilitate environmental remediation, especially by accommodating waste streams. Lignocellulosic waste residues can be transformed into mesoporous biochar structures with substantial pore capacity. While biochars are considered a method of carbon dioxide removal (CDR), they are in fact an environmental double-edged sword that can be used to extract metal ions from water bodies.

View Article and Find Full Text PDF

Many nations are struggling to reduce deforestation, despite having extensive environmental protection laws in place and commitments to international agreements that address the biodiversity and climate crises. We developed a novel framework to quantify the extent to which contemporary deforestation is being captured under national and subnational laws. We then applied this framework to northern Australia as a case study, a development and deforestation hotspot with ecosystems of global significance.

View Article and Find Full Text PDF
Article Synopsis
  • - The mTORC1 pathway is crucial for regulating cell growth and metabolism in response to various environmental signals, particularly amino acids, which activate mTORC1 by influencing Rag GTPases that recruit mTORC1 to the lysosome.
  • - The study found that mTORC1 cannot respond to amino acids in cells without Rag GTPases or the Ragulator component p18, highlighting their role in both mTORC1 activation and the recruitment of associated regulatory complexes (GATOR1, GATOR2, and KICSTOR) to the lysosome.
  • - The findings indicate that the Rag-Ragulator complex is essential for the organization of the mTORC1 nutrient-sensing pathway, emphasizing that mTOR
View Article and Find Full Text PDF

A series of novel hydroxamic acid derivatives was designed and synthesized, and their growth inhibitory activity against bloodstream form was evaluated. These compounds are based on conformationally constrained, lipophilic, spiro carbocyclic 2,6-diketopiperazine (2,6-DKP) scaffolds and bear a side pharmacophoric functionality that contains an acetohydroxamic acid moiety (CHCONHOH) linked with the imidic nitrogen atom of the 2,6-DKP ring via an acetamido portion [CHCON(R), R = H, CH]. Most of these analogues were active in the midnanomolar to low micromolar range against .

View Article and Find Full Text PDF
Article Synopsis
  • Direct methods for assessing DNA polymerase fidelity are straightforward, but measuring RNA polymerases and reverse transcriptases is trickier due to extra preparation steps that can lead to errors.
  • The new method, Roll-Seq, uses single molecule real-time sequencing to evaluate the fidelity of RNA polymerases and reverse transcriptases simultaneously, by generating long concatemeric cDNA from a circular RNA template.
  • Roll-Seq results showed that while some reverse transcriptases had consistent substitution rates, others demonstrated lower fidelity during second-strand synthesis, highlighting the importance of RNA structure on polymerase accuracy.
View Article and Find Full Text PDF

Background: Systemic lupus erythematosus (SLE) is a chronic autoimmune disease with an unpredictable course of recurrent exacerbations alternating with more stable disease. SLE is characterized by broad immune activation and autoantibodies against double-stranded DNA and numerous proteins that exist in cells as aggregates with nucleic acids, such as Ro60, MOV10, and the L1 retrotransposon-encoded ORF1p.

Results: Here we report that these 3 proteins are co-expressed and co-localized in a subset of SLE granulocytes and are concentrated in cytosolic dots that also contain DNA: RNA heteroduplexes and the DNA sensor ZBP1, but not cGAS.

View Article and Find Full Text PDF

Chagas disease is a zoonosis caused by the protozoan parasite Trypanosoma cruzi. Clinical outcomes range from long-term asymptomatic carriage to cardiac, digestive, neurological and composite presentations that can be fatal in both acute and chronic stages of the disease. Studies of T.

View Article and Find Full Text PDF

DNA base damage is a major source of oncogenic mutations. Such damage can produce strand-phased mutation patterns and multiallelic variation through the process of lesion segregation. Here we exploited these properties to reveal how strand-asymmetric processes, such as replication and transcription, shape DNA damage and repair.

View Article and Find Full Text PDF

Selection of nursery habitats by marine fish, such as European sea bass (Dicentrarchus labrax), is poorly understood. Identifying and protecting the full range of juvenile nursery habitats is vital to supporting resilient fish populations and economically important fisheries. We examined how the condition, stomach fullness, and diet of juvenile European sea bass, along with their abundance, differ at high or low tide between the following estuarine habitats: saltmarsh, oyster reefs, shingle, sand, and mud edge habitats.

View Article and Find Full Text PDF

Introduction: Literature shows failure of the outpatient clinic (OC) pathway after emergency department (ED) ultrasound diagnosis of symptomatic cholelithiasis (SC). We hypothesized SC to be more prevalent on final surgical pathology (FSP) in patients who successfully completed OC pathway.

Methods: This retrospective single-institution chart review compared OC and ED patients with right upper quadrant (RUQ) pain and cholelithiasis whom underwent cholecystectomy.

View Article and Find Full Text PDF

Chagas disease is caused by Trypanosoma cruzi, a protozoan parasite that displays considerable genetic diversity. Infections result in a range of pathological outcomes, and different strains can exhibit a wide spectrum of anti-parasitic drug tolerance. The genetic determinants of infectivity, virulence and therapeutic susceptibility remain largely unknown.

View Article and Find Full Text PDF
Article Synopsis
  • Digestive Chagas disease (DCD) is caused by Trypanosoma cruzi infection, and the study aims to explore its pathology and treatment options using a mouse model.
  • Treatment with the anti-parasitic drug benznidazole can lead to recovery in gastrointestinal function if administered early, but delayed treatment results in relapsed symptoms and permanent tissue damage.
  • The research emphasizes the importance of early diagnosis and treatment of T. cruzi infection to prevent DCD, suggesting that even asymptomatic individuals could benefit from treatment with benznidazole.
View Article and Find Full Text PDF

DNA base damage is a major source of oncogenic mutations and disruption to gene expression. The stalling of RNA polymerase II (RNAP) at sites of DNA damage and the subsequent triggering of repair processes have major roles in shaping the genome-wide distribution of mutations, clearing barriers to transcription, and minimizing the production of miscoded gene products. Despite its importance for genetic integrity, key mechanistic features of this transcription-coupled repair (TCR) process are controversial or unknown.

View Article and Find Full Text PDF

How chronic mutational processes and punctuated bursts of DNA damage drive evolution of the cancer genome is poorly understood. Here, we demonstrate a strategy to disentangle and quantify distinct mechanisms underlying genome evolution in single cells, during single mitoses and at single-strand resolution. To distinguish between chronic (reactive oxygen species (ROS)) and acute (ultraviolet light (UV)) mutagenesis, we microfluidically separate pairs of sister cells from the first mitosis following burst UV damage.

View Article and Find Full Text PDF
Article Synopsis
  • Laparoscopic pancreaticoduodenectomy (PD) is gaining popularity, but it has a challenging learning curve that may hinder surgeons moving to new institutions.
  • A study reviewed laparoscopic PD surgeries from two institutions, comparing outcomes and learning curves for the same surgeon at both locations.
  • Results showed that the surgeon achieved the same skill level much faster at the new institution, indicating that specialized surgical techniques can be effectively transferred without losing performance quality.
View Article and Find Full Text PDF

A hallmark of cancer is the avoidance of immune destruction. This process has been primarily investigated in locally advanced or metastatic cancer; however, much less is known about how pre-malignant or early invasive tumours evade immune detection. Here, to understand this process in early colorectal cancers (CRCs), we investigated how naive colon cancer organoids that were engineered in vitro to harbour Apc-null, Kras and Trp53-null (AKP) mutations adapted to the in vivo native colonic environment.

View Article and Find Full Text PDF

The uterus is transiently receptive for embryo implantation. It remains to be understood why the uterus does not reject a semi-allogeneic embryo (to the biological mother) or an allogeneic embryo (to a surrogate) for implantation. To gain insights, we examined uterine early response genes approaching embryo attachment on day 3 post coitum (D3) at 22 hours when blue dye reaction, an indication of embryo attachment, had not manifested in mice.

View Article and Find Full Text PDF

A PHP Error was encountered

Severity: Notice

Message: fwrite(): Write of 34 bytes failed with errno=28 No space left on device

Filename: drivers/Session_files_driver.php

Line Number: 272

Backtrace:

A PHP Error was encountered

Severity: Warning

Message: session_write_close(): Failed to write session data using user defined save handler. (session.save_path: /var/lib/php/sessions)

Filename: Unknown

Line Number: 0

Backtrace: