Highly Multiplexed Single-Cell In Situ Protein Analysis with Cleavable Fluorescent Antibodies.

Limitations on the number of proteins that can be quantified in single cells in situ impede advances in our deep understanding of normal cell physiology and disease pathogenesis. Herein, we present a highly multiplexed single-cell in situ protein analysis approach that is based on chemically cleavable fluorescent antibodies. In this method, antibodies tethered to fluorophores through a novel azide-based cleavable linker are utilized to detect their protein targets.