Mechanism of Intracellular Elemental Sulfur Oxidation in , Where Persulfide Dioxygenase Plays a Key Role.

Representatives of the colorless sulfur bacteria of the genus use reduced sulfur compounds in the processes of lithotrophic growth, which is accompanied by the storage of intracellular sulfur. However, it is still unknown how the transformation of intracellular sulfur occurs in representatives. Annotation of the genome of D-402 did not identify any genes for the oxidation or reduction of elemental sulfur.

Phylogeny and Metabolic Potential of New Giant Sulfur Bacteria of the Family from Coastal-Marine Sulfur Mats of the White Sea.

The family is currently represented by 25 genera in the Genome Taxonomy Database, of which only 6 have a definite taxonomic status. Two metagenome-assembled genomes (MAGs), WS_Bin1 and WS_Bin3, were assembled from metagenomes of the sulfur mats coating laminaria remnants in the White Sea. Using the obtained MAGs, we first applied phylogenetic analysis based on whole-genome sequences to address the systematics of , which clarify the taxonomy of this family.

and : New Molecular Markers for Phylogenetic and Biodiversity Studies of the Genus .

Currently, the phylogeny of the genus is based on comparative whole genome analysis because of the high homology of the 16S ribosomal RNA gene sequences within the genus. We analyzed the possibility of using various conservative genes as phylogenetic markers for the genus . We found that the levels of similarity of the nucleotide sequences of the tRNA(Ile)-lysidine synthase () and the β subunit of RNA polymerase () genes are in good agreement with the average nucleotide identity (ANI) values between the genomes of various representatives of the genus .

Metagenomics Revealed a New Genus ' Thiocaldithrix dubininis' gen. nov., sp. nov. and a New Species ' Thiothrix putei' sp. nov. in the Family , Some Members of Which Have Traits of Both Na- and H-Motive Energetics.

Two metagenome-assembled genomes (MAGs), GKL-01 and GKL-02, related to the family have been assembled from the metagenome of bacterial mat obtained from a sulfide-rich thermal spring in the North Caucasus. Based on average amino acid identity (AAI) values and genome-based phylogeny, MAG GKL-01 represented a new genus within the family. The GC content of the GKL-01 DNA (44%) differed significantly from that of other known members of the genus (50.

History of the Study of the Genus : From the First Enrichment Cultures to Pangenomic Analysis.

Representatives of the genus are filamentous, sulfur-oxidizing bacteria found in flowing waters with counter-oriented sulfide and oxygen gradients. They were first described at the end of the 19th century, but the first pure cultures of this species only became available 100 years later. An increase in the number of described species at the beginning of the 21st century shows that the classical phylogenetic marker, 16S rRNA gene, is not informative for species differentiation, which is possible based on genome analysis.

Correction: Ravin et al. Two New Species of Filamentous Sulfur Bacteria of the Genus , sp. nov. and ' Thiothrix sulfatifontis' sp. nov. 2022, , 1300.

The authors wish to make the following corrections to this paper [...

Two New Species of Filamentous Sulfur Bacteria of the Genus , sp. nov. and ' Thiothrix sulfatifontis' sp. nov.

The metagenome of foulings from sulfidic spring "Serovodorodny" (Tatarstan, Russia), where members of the genus was observed, was sequenced. Representatives of the phyla , and dominated in the microbial community. The complete genome of sp.

Comparative Genome Analysis of the Genus Involving Three Novel Species, sp. nov. Ku-5, sp. nov. AS and " Thiothrix anitrata" sp. nov. A52, Revealed the Conservation of the Pathways of Dissimilatory Sulfur Metabolism and Variations in the Genetic Inventory for Nitrogen Metabolism and Autotrophic Carbon Fixation.

Two strains of filamentous, colorless sulfur bacteria were isolated from bacterial fouling in the outflow of hydrogen sulfide-containing waters from a coal mine ( sp. Ku-5) and on the seashore of the White Sea ( sp. AS).

Reclassification of Sphaerotilus natans subsp. sulfidivorans Gridneva et al. 2011 as Sphaerotilus sulfidivorans sp. nov. and comparative genome analysis of the genus Sphaerotilus.

Filamentous iron oxides accumulating bacteria Sphaerotilus natans subsp. natans and S. natans subsp.

Genomic and Metabolic Insights into Two Novel Species from Enhanced Biological Phosphorus Removal Systems.

Two metagenome-assembled genomes (MAGs), obtained from laboratory-scale enhanced biological phosphorus removal bioreactors, were analyzed. The values of 16S rRNA gene sequence identity, average nucleotide identity, and average amino acid identity indicated that these genomes, designated as RT and SSD2, represented two novel species within the genus , ' Thiothrix moscowensis' and ' Thiothrix singaporensis'. A complete set of genes for the tricarboxylic acid cycle and electron transport chain indicates a respiratory type of metabolism.

Comparative Genomics of Strains D-401 and D-402 with Contrasting Physiology but Extremely High Level of Genomic Identity.

Representatives of filamentous colorless sulfur-oxidizing bacteria often dominate in sulfide biotopes, preventing the diffusion of toxic sulfide into the water column. One of the most intriguing groups is a recently described including strains D-401 and D-402. Both strains have identical genes encoding enzymes which are involved in the oxidation of hydrogen sulfide and thiosulfate.