Publications by authors named "Tatyana Panchenko"
Article Synopsis
- Antibody responses are crucial for defending against SARS-CoV-2 by stopping the virus from entering cells, and a new assay called 2D-MBBA has been developed to measure various antibody isotypes simultaneously.
- This assay was used to analyze IgG, IgM, and IgA levels against the spike protein and its variants, and machine learning significantly improved predictions of how well these antibodies neutralize the virus in convalescent patients.
- The method can differentiate between antibody profiles in convalescent and vaccinated individuals and offers the potential for rapid testing of neutralization efficacy against new variants and pathogens using just a small blood sample.
Article Synopsis
- - Understanding antibody responses to SARS-CoV-2 is crucial for effective COVID-19 containment strategies; however, predicting neutralization capacity remains challenging, as convalescent patients show variable outcomes.
- - A study examined sera from 101 recovered healthcare workers, revealing sustained IgG levels against SARS-CoV-2 proteins, but most individuals had low neutralization ability, with only 6% demonstrating high neutralizing titers.
- - Findings suggest that possessing a variety of antibody isotypes (IgG, IgM, IgA) correlates with stronger neutralization responses, indicating that a broader antibody repertoire may enhance SARS-CoV-2 neutralization effectiveness.
J Immunol Methods
March 2021
Article Synopsis
- The study focuses on developing a cost-effective multiplex bead binding assay (MBBA) for analyzing protein-ligand interactions without the need for specialized equipment or expensive reagents.
- The method innovatively uses biotin-streptavidin interactions and fluorescently labeled magnetic beads to enhance experimental throughput.
- The effectiveness of this new MBBA method is demonstrated by characterizing antibodies against SARS-CoV-2, significantly improving efficiency and reducing the amount of antigen required compared to traditional methods.
Article Synopsis
- * Research on convalescent sera from COVID-19 patients showed that only a small number of antibodies specifically targeted the ACE2-interacting surface on the spike protein, indicating potential immune evasion by the virus.
- * Selection experiments revealed that while unbiased approaches favored antibodies targeting regions away from ACE2IS, biased methods successfully identified ACE2IS-binding antibodies, suggesting that the ACE2IS has evolved to be less immunogenic, which is key for vaccine development.
Article Synopsis
- - The COVID-19 pandemic is a persistent global challenge, where host immunity is crucial for protection, with the spike protein on SARS-CoV-2 being a key target for neutralizing antibodies through its interaction with the ACE2 receptor.
- - Research involving an RBD mutant showed that only a small portion of antibodies from recovered COVID-19 patients targeted the critical ACE2-interacting surface, indicating limited immune recognition of this viral component.
- - Different antibody selection methods revealed that while unbiased selection favored regions outside the ACE2IS, biased selection was effective in identifying ACE2IS-targeting antibodies, suggesting that the ACE2IS is less immunogenic, which is significant for future vaccine development.
Article Synopsis
- ATP-dependent chromatin remodellers help control access to genetic information by altering the positions of nucleosomes, but the way they identify and interact with different nucleosome types is not fully understood.
- A study used a DNA-barcoded mononucleosome library to evaluate the activity of human ISWI family remodellers and found that their accessory subunits can differentiate between nucleosomes with various modifications.
- The research revealed that the nucleosome's acidic patch is crucial for the activity of ISWI, CHD, and SWI/SNF remodellers, indicating that this region serves as an important interaction site that can be modulated by nearby nucleosome modifications.
Nat Chem Biol
December 2016
Article Synopsis
- - Histone covalent modifications, especially new lysine acylations like crotonylation, butyrylation, and propionylation, play a crucial role in epigenetic regulation through 'reader' modules.
- - The double PHD finger (DPF) domains of proteins MOZ and DPF2 show a strong preference for binding to crotonylated histones, as revealed by detailed structural studies.
- - Experiments, including immunofluorescence and ChIP-qPCR, demonstrate that MOZ interacts specifically with crotonylated histones, highlighting a novel regulatory mechanism in gene expression influenced by histone modification.
Article Synopsis
- Histone modifications play a key role in epigenetic regulation, with proteins like bromodomains binding to acetylated lysines, but new non-acetyl modifications like crotonylation are being studied.
- The AF9 YEATS domain has been found to preferentially bind to crotonyllysine instead of acetyllysine, showcasing specific interactions due to its unique structural features.
- Experimental findings indicate that AF9 interacts with crotonylated histone H3 and enhances gene expression, linking crotonylation directly to active transcription processes in cells.
Biotechnol Bioeng
August 2006
Article Synopsis
- Researchers investigated how varying levels of fluorinated amino acids affect the function and stability of an enzyme called chloramphenicol acetyltransferase (CAT).
- They found that replacing all leucine residues with 5',5',5'-trifluoroleucine (TFL) retains enzymatic activity at room temperature and improves secondary structure, but reduces stability when exposed to heat and solvents.
- While the enzyme's catalytic activity remains similar to the wild type, increasing TFL levels leads to decreased stability, indicating that enhanced structure from fluorination doesn’t guarantee better overall stability.
