Biosafety Analysis of Metabolites of Strain 19/97 M, Promising for the Production of Biological Products.

A biosafety study was carried out concerning the metabolites of strain 19/97 M. This strain is a promising producer of biological preparations and shows antagonistic properties against fungi, which cause Fusarium wilt disease. The strain has a pronounced biological activity against conifers, cereals and legumes.

Cellulose-based scaffolds for fluorescence lifetime imaging-assisted tissue engineering.

Quantitative measurement of pH and metabolite gradients by microscopy is one of the challenges in the production of scaffold-grown organoids and multicellular aggregates. Herein, we used the cellulose-binding domain (CBD) of the Cellulomonas fimi CenA protein for designing biosensor scaffolds that allow measurement of pH and Ca gradients by fluorescence intensity and lifetime imaging (FLIM) detection modes. By fusing CBD with pH-sensitive enhanced cyan fluorescent protein (CBD-ECFP), we achieved efficient labeling of cellulose-based scaffolds based on nanofibrillar, bacterial cellulose, and decellularized plant materials.