Publications by authors named "Tatsuyoshi Higashimoto"
Article Synopsis
- Skeletal muscles can adapt and regenerate in response to injury or mechanical stress, but the specific cellular mechanisms behind this adaptation are not well understood.
- Researchers used single-cell RNA sequencing to analyze gene expression in overloaded muscles and found that the cellular makeup of overloaded muscles closely resembled that of regenerating muscles, especially during macrophage activity.
- They discovered that granulin, a factor produced by macrophages, plays a significant role in inhibiting muscle satellite cell differentiation, and granulin-knockout mice had reduced muscle growth due to premature differentiation of those cells.
Skeletal muscle homeostasis and function are ensured by orchestrated cellular interactions among several types of cells. A noticeable aspect of skeletal muscle biology is the drastic cell-cell communication changes that occur in multiple scenarios. The process of recovering from an injury, which is known as regeneration, has been relatively well investigated.
View Article and Find Full Text PDFMuscle satellite cells (MuSCs) supply nuclei to existing myofibers in response to mechanical loading. This myonuclear accretion is critical for efficient muscle hypertrophy. Herein, we present protocols for the detection of MuSC-derived new myonuclei in loaded mouse muscle, including procedures for EdU injection to stain myonuclei, followed by surgery and skeletal muscle fixation.
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