Components of rice husk biochar in promoting the growth, sporulation and iturin A production of Bacillus sp. strain IA.

In a previously study, the Bacillus sp. strain IA was successfully isolated with high sensitivity to rice husk biochar (RHB). Moreover, RHB promoted an antibiotic iturin A production by strain IA.

Promotion of lipopeptide antibiotic production by sp. IA in the presence of rice husk biochar.

The purpose of this study is to isolate the beneficial microorganisms whose growth is promoted in the presence of charcoal materials. We successfully isolated strain IA, whose growth is promoted on an agar plate with charcoal materials, and identified it as a novel strain of the sp. The growth of strain IA in the liquid medium was promoted by the addition of both activated charcoal (AC) and rice husk biochar (RHB).

Isolation of antifungal bacteria from Japanese fermented soybeans, natto.

An inhibitory effect of a traditional Japanese fermented food, natto, was found against plant pathogens such as Rhizoctonia solani and Fusarium oxysporum, and the bacteria which showed inhibition were isolated from the natto. Among isolated bacteria, BC-1 and GAc exhibited a strong antagonistic effect in vitro against plant pathogens on an agar medium. The supernatant of bacterial culture also showed strong activity against R.

Screening of endophytic bacteria against fungal plant pathogens.

Bacterial endophytes were found from 6 plant leaves among 35 plant leaves screened. Two of the isolated bacteria showed antagonistic activity against fungal plant pathogens. An isolate named KL1 showed the clear inihibition against plant pathogens, Fusarium oxysporum and Rhizoctonia solani, on PDA as well as TSA plate.