Alkaline phosphatase (ALP) enhancing iridoid glucosides from the Indonesian medicinal plant Barleria lupulina.

In order to find antiosteoporotic agents from natural resources, 32 Indonesian medicinal plants were screened for their effects on osteoblast differentiation by using alkaline phosphatase (ALP) activity in MC3T3-E1 osteoblast cells as a marker. From the extract of Barleria lupulina, which showed the most potent activity, 13 iridoid glucosides, including three new ones [8-O-acetylipolamiidic acid (1), 8-O-acetyl-6-O-(p-methoxy-cis-cinnamoyl)shanzhiside (2), and 8-O-acetyl-6-O-(p-methoxy-trans-cinnamoyl)shanzhiside (3)] were identified. Among the 13 iridoid glucosides, ipolamiide (4) showed the most potent activity in a dose-dependent manner.

Inhibitory Effect of TNF-alpha Produced by Macrophages Stimulated with Grifola frondosa Extract (ME) on the Growth of Influenza A/Aichi/2/68 Virus in MDCK Cells.

We investigated the inhibitory effect of the conditioned medium (CM) from P338D1 (D1) cells, a murine macrophage cell line, stimulated for 10 hours with a fixed dose (100 mug/ml) of the extracts from the fruit bodies of Grifola frondosa (ME) or its ultra filtration-based fractions (MFs), on the growth of influenza A/Aichi/2/68 virus in Madin-Darby canine kidney cells. Direct addition of ME and 3 kinds of MFs (MF1, MF2 and MF3) to the infected cells had no obvious inhibitory effect. However, virus yields were reduced in the presence of CMs.