Regulation of glycerol metabolism in Enterobacter aerogenes NBRC12010 under electrochemical conditions.

Enterobacter aerogenes NBRC12010 was able to ferment glycerol to ethanol and hydrogen gas. Fermentation of glycerol ceased in the stationary phase of growth, and it was activated by electrochemical reactions using thionine as an electron transfer mediator from bacterial cells to an electrode. Using resting cells of E.

Microbial production of hydrogen and ethanol from glycerol-containing wastes discharged from a biodiesel fuel production plant in a bioelectrochemical reactor with thionine.

H(2) and ethanol production from glycerol-containing wastes discharged from a biodiesel fuel production plant by Enterobacter aerogenes NBRC 12010 was demonstrated in bioelectrochemical cells. Thionine as an exogenous electron transfer mediator was reduced by E. aerogenes, and was re-oxidized by a working electrode applied at +0.

Effect of ammonium addition on methanogenic community in a fluidized bed anaerobic digestion.

After immobilization of anaerobes on carbon felt in a fluidized-bed anaerobic digester at an ammonium concentration of 500 mg N/l, the results of real-time PCR analysis indicated that the cell densities of the immobilized methanogens and bacteria increased compared with those of the free-living methanogens and bacteria in the original anaerobically digested sewage sludge, respectively. The results of the clone analysis of the original sludge suggested that the major methanogens were Methanosaeta sp. and the members of the order Methanomicrobiales, and that after immobilization, these were changed to Methanobacterium and Methanosarcina sp.

Removal of 3-chlorobenzoate using granules in the upflow anaerobic sludge blanket method.

The possibility of removing 3-chlorobenzoate from water using granules in the upflow anaerobic sludge blanket (UASB) method without the addition of a dechlorinating culture was studied on a laboratory scale. After three months of operation, the 3-chlorobenzoate concentration in the effluent decreased to less than 1 mg.l(-1).

Simultaneous methanogenesis and phototrophic bacterial growth in relatively dry sewage sludge under light.

Anaerobically digested sewage sludge with a variety of moisture content, namely 81%, 86%, 90% and 98%, were anaerobically cultured at 35 degrees C under light. Phototrophic bacteria grew in the 86% moisture sludge (bacteriochlorophyll a, 0.46 g/L), 90% sludge (bacteriochlorophyll a, 0.

Performance of a fixed-bed reactor packed with carbon felt during anaerobic digestion of cellulose.

The anaerobic digestion of cellulose was assessed in batch and semi-continuous studies using a carbon felt fixed-bed reactor. In the batch operation, the volatile solids reduction (%) and the cumulative methane production during the mesophilic and thermophilic digestion were 52.2% and 15.