Publications by authors named "Tatsuo Maejima"
Nanoparticles are currently attracting considerable attention because of their ability to conjugate to various substances. As such, these nanoparticles can assist the transfer of the conjugated substance to target tissues where they are gradually released. In this study, vancomycin-conjugated nanoparticles (VCM NPs) were prepared.
View Article and Find Full Text PDFWe established a mesenchymal stem cell clone, 5F9A, from rat bone marrow substrate adherent cells by repeated limiting dilutions. The cells have a fibroblastic shape and form intimate contacts with adjacent cells with interdigitations and junctions similar to adherence and tight junctions in a semi-confluent culture. Analysis of the phenotypes of these cells by RT-PCR and FACS demonstrated that they resembled mesenchymal stem cells, and the cells could differentiate into adiopocytes and osteoblasts under appropriate conditions in vitro showing their oligopotency.
View Article and Find Full Text PDFArticle Synopsis
- The 5F9A cell, a type of mesenchymal stem cell-like clone from rat bone marrow, can develop into fat cells (adipocytes) and bone-forming cells (osteoblasts) under the right conditions.
- The formation of multinucleated cells in 5F9A was induced by TPA and was largely driven by protein kinase C, suggesting two possible processes: karyokinesis without cytokinesis or cell fusion.
- Observations showed that most multinucleated cells arose from karyokinesis without cytokinesis, while cell fusion contributed to a lesser extent, confirmed through advanced microscopy techniques.