Publications by authors named "Tatsuki Takakura"

The metabolic stages of bacterial development and viability under different stress conditions induced by disinfection, chemical treatments, temperature, or atmospheric changes have been thoroughly investigated. Here, we aim to evaluate early metabolic modifications in bacteria following induced stress, resulting in alterations to bacterial metabolism. A protocol was optimized for bacterial preparation using energy-dispersive X-ray (EDX) microanalysis coupled with scanning electron microscopy (SEM), followed by optimizing EDX data acquisition and analysis.

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Enabling faster Antimicrobial Susceptibility Testing (AST) is critical, especially to detect antibiotic resistance, to provide rapid and appropriate therapy and to improve clinical outcomes. Although several standard and automated culture-based methods are available and widely used, these techniques take between 18 and 24 h to provide robust results. Faster techniques are needed to reduce the delay between test and results.

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Blood culture is currently the most commonly used method for diagnosing sepsis and bloodstream infections. However, the long turn-around-time to achieve microbe identification remains a major concern for clinical microbiology laboratories. Gram staining for preliminary identification remains the gold standard.

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Article Synopsis
  • Infectious endocarditis (IE) is a serious heart disease associated with high mortality, often due to thrombo-embolic events.
  • Current treatment involves surgery and antibiotics, but complications persist due to limited understanding of cell organization in vegetations caused by different bacteria.
  • The study utilized scanning electron microscopy and energy-dispersive X-ray spectroscopy to analyze infected heart valve tissue, revealing differences in cell organization and chemical features that could help tailor therapies based on the specific infection.
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