Publications by authors named "Tatjana Novikova"
Primary cultures of rat hepatocytes were studied in serum-free medium. Ultradian protein synthesis rhythm was used as a marker of overall cell synchronization and cooperation amongst the population. The level of synchronization was determined by amplitudes of the rhythm.
View Article and Find Full Text PDFThe authors present their clinical experience in reconstruction of soft-tissue defects of the scalp in 17 patients, using free radial forearm perforator flaps. These defects, as a rule, have a circular or elliptical shape. In order to fit this shape of the defect, they divided the radial forearm perforator flap into two or three components in each case.
View Article and Find Full Text PDFReconstruction of soft tissue defects of the hand using the shape-modified radial forearm flap.
Scand J Plast Reconstr Surg Hand Surg
April 2005
We have reconstructed soft tissue defects in 121 hands with radial forearm flaps. So that the flap perfectly fitted the defect, and to minimise the size of the donor site, we divided the flap into two or three components in each case. We call this the shape-modified radial forearm flap.
View Article and Find Full Text PDFArticle Synopsis
- Ultradian oscillations in protein synthesis indicate how hepatocytes (liver cells) can work together rhythmically, with stronger rhythms linked to better cell cooperation.
- Experiments with different cultures of young and old rat hepatocytes showed younger cells generally had more pronounced rhythms, but environmental factors like the medium composition can significantly impact the results.
- The addition of specific substances like gangliosides or phenylephrine could enhance the protein synthesis rhythms in older cells to levels seen in younger ones, suggesting that the cell environment plays a crucial role in cooperation more than just age.
Article Synopsis
- The study investigates the role of ultradian protein synthesis rhythms as indicators of cooperation among liver cells (hepatocytes) in different culture conditions.
- Phenylephrine and a specific compound (2,5-di(tertiary-butyl)-1,4-benzohydroquinone) were found to stimulate cooperative behavior in sparse cultures by increasing intracellular calcium levels ([Ca(2+)](cyt)).
- The experiment shows that disrupting calcium signaling with BAPTA-AM abolished the protein synthesis rhythm, highlighting the importance of calcium and gangliosides (like GM1) in synchronizing cell activities and modulating protein metabolism.
Pretreatment of hepatocyte cultures with 1 microM d-l-threo-1-phenyl-2-hexadecanoylamino-3-pyrrolidino-1-propanol-HCL (PPPP) for 24 h decreased the ganglioside GM1 content of the cells by approximately 50% and that of the conditioned medium by 90%. No rhythm in the rate of protein synthesis was detected in dense cultures pretreated with PPPP, but was observed in control dense cultures. Conditioned medium from control dense cultures induced synchrony in sparse cultures, which were non-synchronous in their own medium.
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