Publications by authors named "Tatjana E Novikova"
Primary cultures of rat hepatocytes were studied in serum-free medium. Ultradian protein synthesis rhythm was used as a marker of overall cell synchronization and cooperation amongst the population. The level of synchronization was determined by amplitudes of the rhythm.
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- Ultradian oscillations in protein synthesis indicate how hepatocytes (liver cells) can work together rhythmically, with stronger rhythms linked to better cell cooperation.
- Experiments with different cultures of young and old rat hepatocytes showed younger cells generally had more pronounced rhythms, but environmental factors like the medium composition can significantly impact the results.
- The addition of specific substances like gangliosides or phenylephrine could enhance the protein synthesis rhythms in older cells to levels seen in younger ones, suggesting that the cell environment plays a crucial role in cooperation more than just age.
Article Synopsis
- The study investigates the role of ultradian protein synthesis rhythms as indicators of cooperation among liver cells (hepatocytes) in different culture conditions.
- Phenylephrine and a specific compound (2,5-di(tertiary-butyl)-1,4-benzohydroquinone) were found to stimulate cooperative behavior in sparse cultures by increasing intracellular calcium levels ([Ca(2+)](cyt)).
- The experiment shows that disrupting calcium signaling with BAPTA-AM abolished the protein synthesis rhythm, highlighting the importance of calcium and gangliosides (like GM1) in synchronizing cell activities and modulating protein metabolism.