Conformational Effects on the Passive Membrane Permeability of Synthetic Macrocycles.

Macrocyclic compounds (MCs) can have complex conformational properties that affect pharmacologically important behaviors such as membrane permeability. We measured the passive permeability of 3600 diverse nonpeptidic MCs and used machine learning to analyze the results. Incorporating selected properties based on the three-dimensional (3D) conformation gave models that predicted permeability with = 0.

An optimized permeabilization step for flow cytometry analysis of nuclear proteins in myeloid differentiation of blood cells into neutrophils.

Polymorphonuclear leukocytes (PMNLs) or neutrophils play an important role in the innate immune response. Working with human neutrophils is challenging because these cells are sensitive to changes in the surrounding media and quickly become apoptotic. Meanwhile the experiments with mature neutrophils may be very important for studies of blood function.

[New alkyl cationic glycerolipids with heterocyclic polar domain are responsible for disorders in the leukemia cells cycle and cells apoptosis].

A series of new modifications of alkyl cationic glycerolipids with hetero polar domain has been synthesized. The most active compound rac-N-(4-[(2-ethoxy-3-octadecyloxy)prop-1-yloxycarbonyl]butyl)-N'-etylimidazoliyiodid in micromolar concentrations causes a delay of cell cycle in phase G1, DNA fragmentation and apoptosis of cell line leukemia.

Oceanographic lidar attenuation coefficients and signal fluctuations measured from a ship in the Southern California Bight.

We measured the attenuation coefficient of the National Oceanic and Atmospheric Administration lidar from a ship in the Southern California Bight in September 1995. The region from approximately 5 to 30 m in depth was covered. The laser was linearly polarized, and the receiver was operated with the same polarization and the orthogonal polarization.

[Procedure for determination of individual sensitivity to antitumor drugs].

The present paper proposes to employ the cultured tumor cells of the breast and chick fibroblasts after long-term cultivation (for above 24 days) to determine their individual drug sensitivity and, as a criterion of cell damage, to use the percent of destruction of the cell layer formed in the wells 24 hours after drug insertion. It also presents the comparative results of tests of 2 cellular models that have been used to determine the in vitro sensitivity of the cells of breast cancer and chick fibroblasts to melfalan and its complex compound with copper acetylacetonate - MOK*M. At the same time, the cytotoxic activity of MOK*M and melfalan against tumor cells has been not shown to differ greatly (16.

Lidar profiles of fish schools.

A lidar system was used in a seawater tank to measure the average diffuse reflectivity of live sardines. Diffuse reflectivity was measured to be 10 % for a copolarized laser return and 3 % for a cross-polarized return. We used these calibration measurements to infer the density of sardines in areas of the Southern California Bight from vertical profiles obtained with the lidar mounted on a ship.

Polarization of light scattered from slightly rough dielectric film.

We found that a slightly rough thin dielectric film polarizes diffusely scattered light. There is a discrete set of angles of incidence and observation for which the intensity of a nonspecular diffuse P-polarized component is significantly greater than that for S polarization.

[Evaluation of the level of cancerogenic waste in energetics and calculation of its effect on air pollution].

Investigations showed that heat plants pollute atmospheric air in towns of Khabarovsk district. Concentrations of benzo(a)pyrene in air of Khabarovsk, Komsomolsk-on-Amur, and Amursk are 4-14 times higher than its maximum allowable concentration.

[Ion conductance channels of bilayer from sarcoma-45 phospholipids].

Properties of black bilayer membranes formed from sarcoma-45 phospholipids differed from those of normal cell phospholipids bilayers. Two types of Ca2+-channels were discovered: those multiple to 35 pA and those multiple to 90 pA. The study of bilayers from phospholipids of nuclei, mitochondria, microsomal fractions showed that Ca2+-channels were localized in the microsomal fraction of transformed cells.