Publications by authors named "Tat'yana G Smirnova"
A MALDI TOF MS based minisequencing method has been developed and applied for the analysis of rifampin (RIF)- and isoniazid (INH)-resistant M. tuberculosis strains. Eight genetic markers of RIF resistance-nucleotide polymorphisms located in RRDR of rpoB gene, and three of INH resistance including codon 315 of katG gene and -8 and -15 positions of the promoter region of fabG1-inhA operon were worked out.
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- Three genetic loci of Mycobacterium tuberculosis (rpoB, katG, and the fabG1(mabA)-inhA operon) were analyzed to understand mutations linked to rifampicin and isoniazid resistance.
- A total of 412 M. tuberculosis isolates from Russia (1997-2005) were examined using a MALDI-TOF MS minisequencing technique to identify these mutations.
- The study revealed multiple mutations in the rpoB gene, with specific codons showing high variability; additionally, most isoniazid-resistant isolates exhibited mutations in the katG gene, indicating the potential for using this rapid method to track drug resistance.