Synergistic RAS-MAPK and AKT Activation in MYC-Driven Tumors via Adjacent PVT1 Rearrangements.

MYC-driven (MYC+) cancers are aggressive and often fatal. MYC dysregulation is a key event in these cancers, but overexpression of MYC alone is not always enough to cause cancer. (), a long non-coding RNA (lncRNA) adjacent to MYC on chromosome 8 is a rearrangement hotspot in many MYC+ cancers.

Locations of metastases in and oncological outcomes of patients with metastatic castration-sensitive prostate cancer: Real-world data from a multicenter study.

Background: Androgen receptor-signaling inhibitors (ARSIs) have significantly changed the preferred treatments for metastatic castration-sensitive prostate cancer (mCSPC). Despite such advances, the prognostic significance of metastases at specific sites remains unclear. This study evaluated how metastatic site affected the oncological outcomes of mCSPC patients.

Novel Preoperative Immune Prognostic Index for Predicting Outcomes in Patients Undergoing Radical Nephroureterectomy for Upper Tract Urothelial Carcinoma.

Background/aim: The lung immune prognostic index (LIPI), which is determined by assessing the derived neutrophil-to-lymphocyte ratio in conjunction with the level of lactate dehydrogenase, predicts outcomes in various cancers. Its utility as a preoperative biomarker in upper tract urothelial carcinoma (UTUC) patients remains unexplored.

Patients And Methods: This is a retrospective study of UTUC patients who underwent radical nephroureterectomy.

Transcriptional Profiling of Patients With Fuchs Endothelial Corneal Dystrophy With and Without Trinucleotide Repeat Expansion in TCF4.

Purpose: To investigate transcriptomic differences between patients with Fuchs endothelial corneal dystrophy (FECD) with and without trinucleotide repeat (TNR) expansion in the transcription factor 4 gene, as the genetic basis for patients with FECD lacking TNR expansion remains unclear.

Methods: Bulk RNA sequencing was performed on corneal endothelial cells from 17 subjects: 10 patients with FECD [4 with repeat expansion lengths <50 (RE-) and 6 with expansions of 50 or more (RE+)] and 7 controls. Differential gene expression analysis was conducted using DESeq2.

Serological Evidence of Cetacean Morbillivirus Infection in Common Bottlenose Dolphins in Japan.

This study revealed that 41.9% of wild-born Japanese captive dolphins (18 out of 43 dolphins) have neutralizing antibodies specific to cetacean morbillivirus (CeMV). This finding indicates a widespread yet undetected CeMV infection among wild dolphins in the waters around Japan, occurring without notable epidemic or mass mortality of dolphins.

Isepamicin alone as antimicrobial prophylaxis for transrectal prostate needle biopsy: "Do we still need levofloxacin? ".

The purpose of this study was to investigate whether conventional levofloxacin (LVFX) administration is unnecessary for transrectal ultrasound-guided prostate needle biopsy (TRP-Bx) in view of the increase in LVFX-resistant Escherichia coli and appropriate use of antibiotics. The study included 636 cases undergoing TRP-Bx, divided into two groups based on the prophylactic antibiotic regimen. Group 1 (n = 308) received both oral levofloxacin (LVFX) 500 mg and intravenous isepamicin (ISP) 400 mg.

Current Status and Prospects of Regional Collaborative Pathways for Management of Acute Coronary Syndrome in Japan - A Nationwide Survey.

Background: Comprehensive management of acute coronary syndrome (ACS) requires seamless treatment across institutions, including intensive care centers and local clinics. However, maintaining guideline-directed medical therapy remains challenging. One promising option to improve the situation may be the implementation of regional collaborative clinical pathways.

Evidence for successful gastric perfusion computed tomography with low-radiation dose.

Objective: To investigate how the blood flow analysis changes by varying the radiation dose of gastric perfusion CT (PCT) and to prove that a low-radiation dose of PCT is feasible.

Methods: 5 Beagle dogs were used in a crossover study with 6 groups of varying radiation doses. Iodixanol was IV administered at 3.

Raman and ATR-FTIR unmask crystallinity changes and carboxylate group and vinyl group accumulation in natural weathering polypropylene microplastics.

Naturally weathered polypropylene (NWPP) samples are useful for investigating the effects of various degradation factors that cannot be obtained in artificial laboratory experiments. In this study, NWPP samples were extracted from beach sediments (Ashiya, Hyogo, Japan). Raman and attenuated total reflection (ATR)-Fourier-transform infrared (FTIR) spectroscopies were used to analyze variations in the composition, crystallinity, orientation, and degradation of NWPP microplastics.

Evaluation of optimal timing and therapeutic efficacy of radium-223 therapy for metastatic castration-resistant prostate cancer: a multicenter collaborative study.

Background: Despite its demonstrated efficacy in prolonging overall survival (OS) and delaying skeletal-related events in the ALSYMPCA trial, the optimal timing of radium-223 initiation remains unclear. This study investigated factors influencing radium-223 treatment outcomes, including completion rates and survival.

Methods: This retrospective, multi-institutional study included 164 patients with metastatic castration-resistant prostate cancer (CRPC) who received radium-223 therapy.

Clinical outcomes and treatment patterns of maintenance avelumab in locally advanced or metastatic urothelial carcinoma: a multicenter collaborative study.

Background: The JAVELIN Bladder 100 trial demonstrated improved overall survival (OS) with maintenance avelumab in patients with locally advanced or metastatic urothelial carcinoma UC (la/mUC) who achieved disease control following first-line platinum-based chemotherapy (1 L-PBC). However, real-world data on eligibility, utilization, and outcomes of maintenance avelumab therapy remain limited.

Methods: This retrospective study included patients with la/mUC who received 1 L-PBC.

Sex-Dependent Variations in Gene Expression in Corneal Endothelial Cells Among Healthy Individuals and Patients With Fuchs Endothelial Corneal Dystrophy.

Purpose: Fuchs endothelial corneal dystrophy (FECD) displays a higher incidence in females than in males, yet the underlying mechanism remains unclear. This study aimed to elucidate sex-dependent differential gene expressions in corneal endothelial cells (CECs) from healthy non-FECD individuals and from patients with FECD.

Methods: RNA-Seq data from CECs of non-FECD subjects (3 males, 4 females) and FECD subjects (5 males, 5 females) were analyzed to identify differentially expressed genes (DEGs) between the sexes.

Schizophrenia-related Xpo7 haploinsufficiency leads to behavioral and nuclear transport pathologies.

Recent genetic studies by the Schizophrenia Exome Sequencing Meta-Analysis (SCHEMA) consortium have identified that protein-truncating variants of exportin 7 (XPO7) can increase the risk of schizophrenia (odds ratio, 28.1). Here we show that mice with Xpo7 haploinsufficiency (Xpo7 mice) present with cognitive and social behavioral impairments.

Use of Direct Oral Anticoagulation for Isolated Distal Deep Vein Thrombosis in Japanese Orthopedic Patients.

Although direct oral anticoagulants (DOAC) have become widely used, little is known about the efficacy of DOAC for isolated distal deep vein thrombosis (DVT). In-hospitalized orthopedic patients with isolated distal DVT who were diagnosed from 2016 to 2018 were enrolled and were followed for 1 year. Embolic events included symptomatic pulmonary embolism (PE) and DVT extension above the knee.

Predicting pathological upstaging after radical nephroureterectomy in patients with upper tract urothelial carcinoma: results from a multicenter cohort study.

Background: Despite the availability of advanced imaging technologies, it remains difficult to achieve sufficient staging accuracy to ensure a tailored treatment strategy for patients with upper tract urothelial carcinoma (UTUC). The aim of the study was to identify preoperative risk factors for tumor upstaging in patients with UTUC initially staged as clinical T2 or lower and to analyze these factors separately for renal pelvic cancer and ureteral cancer.

Methods: This retrospective study included data from patients with UTUC who underwent nephroureterectomy.

The Mechanism and Management of Pneumopericardium Caused by Right Ventricular Lead Perforation.

An 83-year-old man underwent dual-chamber pacemaker placement for complete atrioventricular block at another hospital. The active-fixation ventricular lead was positioned on the free wall of the anterior right ventricle. Ventricular pacing failure occurred on the day after pacemaker implantation, and fluoroscopy revealed right ventricular (RV) lead perforation.

Pitfalls in the analysis conditions of canine brain perfusion computed tomography.

This study aimed to investigate the impact of selected analysis conditions on blood flow values and color maps in canine brain perfusion computed tomography (PCT) and to propose optimal analysis conditions. Dynamic computed tomography imaging was performed on six beagle dogs. Color maps were generated using a combination of analysis algorithms (box-modulation transfer function (Box-MTF) and singular value deconvolution plus (SVD+) methods), slice thicknesses (4.

Improved genome sequence of GBlys, a lysogenic strain by lysogenic phage phiOH2.

GBlys is a lysogenic strain of the temperate phage phiOH2, which is 3,644,428 bp long, has a GC content of 52%, and contains 3,595 predicted protein-coding genes. Here, we report a resequenced GBlys genome obtained by deep sequencing with long and short reads.

Canine regional gastric blood flow measurement using perfusion computed tomography.

This study aimed to determine the feasibility of using perfusion computed tomography (CT) to assess blood flow in different regions of the stomach in dogs. Dynamic perfusion CT scans were conducted on five beagle dogs, and blood flow analysis was performed using the maximum slope and Patlak plot methods. The findings revealed significant variations in blood flow among the fundus, body, and pylorus of the stomach.

Comparison of Hemodynamic Effects of Remimazolam and Midazolam During Anesthesia Induction in Patients Undergoing Cardiovascular Surgery: A Single-Center Retrospective and Exploratory Study.

Introduction: Patients undergoing cardiovascular surgery may experience hemodynamic instability during the induction of general anesthesia, and anesthetic agents with minimal hemodynamic effects should be administered. Midazolam, a classic benzodiazepine anesthetic, is known to have relatively weak circulatory depression during anesthesia induction compared to other sedatives. On the other hand, remimazolam, a newly approved short-acting benzodiazepine anesthetic, is expected to have fewer circulatory depressant effects.

Localization of Both CD31- and Endomucin-Expressing Vessels in Mouse Dental Pulp.

We investigated the localization of both CD31- and endomucin-expressing vessels in mouse dental pulp to elucidate their relationship with dentin formation. The maxillae of C57BL/6 male mice (1, 4, 8, 12, and 56 weeks old) were fixed with 4% paraformaldehyde solution, and cryosections (12-μm-thick) were prepared. Immunofluorescence was performed using anti-CD31 and anti-endomucin antibodies, and calcein labeling was conducted to elucidate relationships with dentin formation.

Submucosal hemorrhage of the esophagus: a case report.

Background: Submucosal hemorrhage of the esophagus is relatively rare and the course of this disease remains unclear. We report a case of this disease.

Case Presentation: The patient was a 68-year-old man who visited a clinic complaining of sudden-onset epigastric and back pain.

Successful treatment of esophageal perforation due to black esophagus (acute esophageal necrosis): a case report.

Background: Black esophagus, or acute esophageal necrosis (AEN), is a rare disease with a poor prognosis in which the esophageal mucosa is black in color. We report a case of esophageal perforation due to AEN that was successfully treated.

Case Presentation: An 88-year-old woman presented to her local hospital with the chief complaint of abdominal pain.

Molecular mechanisms of complex-type N-glycan breakdown and metabolism by the human intestinal bacterium Barnesiella intestinihominis.

Intestinal bacteria play a crucial role in human health, for example, by maintaining immune and metabolic homeostasis and protecting against pathogens. Survival in the human intestine depends on the bacterium's ability to utilize complex carbohydrates. Some species are known to use host-derived glycans; for example, Bifidobacteria can utilize O-glycan of mucin.