Defining the term "underserved:" A scoping review towards a standardized description of inadequate access to veterinary services.

This scoping review aims to establish a comprehensive definition of the term "underserved" as it applies to communities, individuals, and populations with inadequate access to animal health services, particularly for dogs. The review adhered to PRISMA guidelines and analyzed 30 articles, applying concepts of One Health and social determinants of health, by using 3 pre-determined categories of contributors to and indicators of underservice. The review categorized article-specific exemplars into veterinary-dependent barriers; community- and individual-related barriers; and health and welfare indicators; with subcategories illustrating features of underserved communities, individuals, or populations in each category.

Developing a framework for a western Canadian companion animal surveillance initiative: Case definitions and the role of the veterinarian.

Objective: Surveillance data on companion animals in western Canada are extremely limited. Previous research by the principal investigators established a list of potential canine pathogens of relevance to public health for inclusion in the Western Canadian Companion Animal Surveillance Initiative (CASI). Our objective was to assess veterinary interest in contributing to companion animal surveillance, and to gather baseline data on specific canine pathogens of interest to create surveillance-specific case definitions.

Understanding and strengthening wildlife and zoonotic disease policy processes: A research imperative.

The COVID-19 pandemic highlights the urgency and importance of monitoring, managing and addressing zoonotic diseases, and the acute challenges of doing so with sufficient inter-jurisdictional coordination in a dynamic global context. Although wildlife pathogens are well-studied clinically and ecologically, there is very little systematic scholarship on their management or on policy implications. The current global pandemic therefore presents a unique social science research imperative: to understand how decisions are made about preventing and responding to wildlife diseases, especially zoonoses, and how those policy processes can be improved as part of early warning systems, preparedness and rapid response.

Investigating SARS-CoV-2 Susceptibility in Animal Species: A Scoping Review.

In the early stages of response to the SARS-CoV-2 pandemic, it was imperative for researchers to rapidly determine what animal species may be susceptible to the virus, under low knowledge and high uncertainty conditions. In this scoping review, the animal species being evaluated for SARS-CoV-2 susceptibility, the methods used to evaluate susceptibility, and comparing the evaluations between different studies were conducted. Using the PRISMA-ScR methodology, publications and reports from peer-reviewed and gray literature sources were collected from databases, Google Scholar, the World Organization for Animal Health (OIE), snowballing, and recommendations from experts.

Establishment of apiary-level risk of American foulbrood through the detection of Paenibacillus larvae spores in pooled, extracted honey in Saskatchewan.

Paenibacillus larvae, the causative agent of American foulbrood (AFB), produces spores that may be detectable within honey. We analyzed the spore content of pooled, extracted honey from 52 large-scale (L) and 64 small-scale (S) Saskatchewan beekeepers over a two-year period (2019-2020). Our objectives were: (i) establish reliable prognostic reference ranges for spore concentrations in extracted honey to determine future AFB risk at the apiary level; (ii) identify management practices as targets for mitigation of risk.

Comparison of individual hive and apiary-level sample types for spores of Paenibacillus larvae in Saskatchewan honey bee operations.

Three commercial honey bee operations in Saskatchewan, Canada, with outbreaks of American foulbrood (AFB) and recent or ongoing metaphylactic antibiotic use were intensively sampled to detect spores of Paenibacillus larvae during the summer of 2019. Here, we compared spore concentrations in different sample types within individual hives, assessed the surrogacy potential of honey collected from honey supers in place of brood chamber honey or adult bees within hives, and evaluated the ability of pooled, extracted honey to predict the degree of spore contamination identified through individual hive testing. Samples of honey and bees from hives within apiaries with a recent, confirmed case of AFB in a single hive (index apiaries) and apiaries without clinical evidence of AFB (unaffected apiaries), as well as pooled, apiary-level honey samples from end-of-season extraction, were collected and cultured to detect and enumerate spores.

The epidemiology and economics of pooled testing for disease investigations of lead exposure involving beef cattle in Saskatchewan (2007-2019).

From 2007 to 2019, the Western College of Veterinary Medicine Disease Investigation Unit conducted lead investigations in 12 beef cow-calf herds and tested 1104 presumed exposed but clinically unaffected cattle, 49 of which were unsafe for slaughter (blood lead ≥ 0.1 ppm). In all investigations, the lead source should be evaluated, and all potentially exposed animals intended for food should be tested.

IIB or not IIB, part 2: assessing inter-rater and intra-rater repeatability of the Kenney-Doig scale in equine endometrial biopsy evaluation.

Inter- and intra-rater variability negatively affects the reliability of various histopathology grading scales used as prognostic aids in human and veterinary medicine. The Kenney-Doig categorization (grading) scale, which is used to associate equine endometrial histologic lesions with prognostic estimation of a broodmare's reproductive potential, has not been evaluated for inter- or intra-rater variability, to our knowledge. To assess whether the Kenney-Doig system produces reliable results among observers, 8 pathologists, all with American College of Veterinary Pathologists certification, were recruited to blindly categorize the same set of 63 digital equine endometrial biopsy slides as well as to re-evaluate anonymously 21 of 63 of these slides at a later time.

IIB or not IIB, part 1: retrospective evaluation of Kenney-Doig categorization of equine endometrial biopsies at a veterinary diagnostic laboratory and comparison with published reports.

The Kenney-Doig scale is a histopathology categorization (grading) system often used as the standard for assessing endometrial disease and communicating prognostic fertility information for equine breeding prospects. We investigated how Kenney-Doig categories compared within the same institution and across different institutions to determine if observer variability may contribute to category frequencies. We conducted a retrospective analysis of all equine endometrial submission records between 1998 and 2018 at the Western College of Veterinary Medicine (WCVM) and Prairie Diagnostic Services (PDS).

Defining important canine zoonotic pathogens within the Prairie Provinces of Canada.

The goal of this study was to establish a short list of zoonotic pathogens involving the domestic dog that can be prioritized for a companion animal surveillance program specific to the Prairie Provinces of Canada. A list of pathogens documented in dogs was created through a comprehensive review of infectious disease textbooks for the following taxonomical categories: bacteria, ectoparasites, fungi, helminths, protozoa, rickettsia, and viruses. This created an initial list of 594 pathogens that was then pared down through an extensive review of the literature using the following criteria: i) the pathogen is zoonotic/sapronotic/anthroponotic; ii) the dog is involved in transmission to humans, maintenance, or detection of the pathogen; and iii) there is a level of risk for occurrence of the pathogen in Canada.

Traceback of the outbreak in British Columbian bighorn sheep ().

are a non-burrowing, ectoparasitic, mange-causing mite that has been documented in American bighorn sheep populations throughout the 19th and 20th centuries; however, it was not seen on Canadian bighorn sheep until 2006. The aim of this study was to determine the potential source of the outbreak in Canadian bighorn sheep. Morphological and molecular analyses were used to compare mites recovered from outbreak-associated bighorn sheep, pet rabbits in Canada, and on historically infested bighorn sheep in the USA.

Investigation of clinical outbreaks of American foulbrood in honey-bee operations in Saskatchewan.

Four outbreaks of American foulbrood were investigated in honey-bee operations in Saskatchewan during the summer of 2019. Clinical signs were confirmed by the Saskatchewan Provincial Specialist in Apiculture and the causative agent was cultured and identified through matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS). Evaluation of management practices revealed off-label metaphylactic use of oxytetracycline in 3 of 4 operations and a discontinuation of antibiotic use in the fourth.

Prevalence and Prognostic Impact of Papillomavirus Type 2 Infection in Equine Squamous Cell Carcinomas in Western Canadian Horses.

papillomavirus type-2 (EcPV-2) has been proposed as a causal factor in equine genital squamous cell carcinoma (SCC). This study had 2 objectives: first, calculate the frequency of papillomavirus (PV) and EcPV-2 infection in papillomas, carcinomas in situ (CIS), and SCCs in Western Canadian horses; and second, determine if EcPV-2 status of equine SCCs is associated with overall survival (OS). EcPV-2 status of 115 archived tissue samples, spanning 6 years, was determined using broad spectrum (MY09/11) and EcPV-2-specific polymerase chain reaction (PCR) assays, EcPV-2-E6/E7 chromogenic RNA in situ hybridization (R-ISH), and amplicon sequencing.

Effects of the Glucosinolate Sinigrin in Combination With a Noniodine Supplemented Diet on Serum Iodine and Thyroid Hormone Concentrations in Nonpregnant Mares.

Exposure to plants containing glucosinolates (GSLs) affects thyroid function in many species, in horses is implicated in the birth of foals with congenital hypothyroidism. The present study was performed to determine the effect of feeding a GSL (sinigrin) in combination with a low-iodine diet for 12 weeks on thyroid hormones and serum iodine concentrations in nonpregnant mares. Nineteen mares aged 2-14 years were divided into control (n = 6), low (20 mmol/day) (n = 7) and high GSL (35 mmol/day) (n = 6) groups.

A Field Study of Serum, Colostrum, Milk Iodine, and Thyroid Hormone Concentrations in Postpartum Draft Mares and Foals.

Iodine, thyroxine (T4) and triiodothyronine (T3) are required for normal fetal growth, maturation, and neonatal survival. There is a lack of robust information on iodine levels found in colostrum, milk, and serum of mares and foals after a healthy pregnancy. Our objective was to characterize colostrum, milk, and serum iodine levels in healthy postpartum mares and foals (n = 10) and explore relationships with thyroid hormone concentrations.

Effects of Pesticides on European Foulbrood in Honeybee Larvae.

Neonicotinoid and fungicide exposure has been linked to immunosuppression and increased susceptibility to disease in honeybees (). European foulbrood, caused by the bacterium , is a disease of honeybee larvae which causes economic hardship for commercial beekeepers, in particular those whose colonies pollinate blueberries. We report for the first time in Canada, an atypical variant of isolated from a blueberry-pollinating colony.

Chronic High-Dose Neonicotinoid Exposure Decreases Overwinter Survival of L.

Overwinter colony mortality is an ongoing challenge for North American beekeepers. During winter, honey bee colonies rely on stored honey and beebread, which is frequently contaminated with the neonicotinoid insecticides clothianidin and thiamethoxam. To determine whether neonicotinoid exposure affects overwinter survival of L.

Disease investigations for equine infectious anemia in Canada (2009-2012) - Retrospective evaluation and risk factor analysis.

This retrospective study describes the detection of equine infectious anemia (EIA) during Canadian Food Inspection Agency (CFIA) disease investigations in Canada, examines aspects of importance for disease control, and evaluates potential animal-level risk factors for EIA in high-risk horses. Based on review of all EIA-positive samples and all samples collected during disease investigations (N = 4553) over a 4-year period (2009 to 2012), 409 EIA cases were detected. Horse owners with EIA cases owned between 1 and 60 affected animals, and 49 horses seroconverted during a disease investigation period.

Tuberculosis in Canada and the United States: a review of trends from 1953 to 2015.

Objectives: To explore tuberculosis (TB) incidence in Canada and the United States from 1953 to 2015. In the most recent decade, the US incidence was lower than that of Canada. Since both countries are high income and have low TB incidence with similar TB surveillance programs, we hypothesized that rates should be similar.

Effects of chronic dietary thiamethoxam and prothioconazole exposure on Apis mellifera worker adults and brood.

Background: Chronic exposure of honey bees (Apis mellifera Linnaeus) to the neonicotinoid thiamethoxam and the fungicide prothioconazole is common during foraging in agricultural landscapes. We evaluated the survival and hypopharyngeal gland development of adult worker honey bees, and the survival of the worker brood when chronically exposed to thiamethoxam or thiamethoxam and prothioconazole in combination.

Results: We found that 30 days of exposure to 40 μg kg of thiamethoxam significantly (P < 0.

The global distribution of Bacillus anthracis and associated anthrax risk to humans, livestock and wildlife.

Bacillus anthracis is a spore-forming, Gram-positive bacterium responsible for anthrax, an acute infection that most significantly affects grazing livestock and wild ungulates, but also poses a threat to human health. The geographic extent of B. anthracis is poorly understood, despite multi-decade research on anthrax epizootic and epidemic dynamics; many countries have limited or inadequate surveillance systems, even within known endemic regions.

Bayesian Hierarchical Models as Tools to Evaluate the Association Between Groundwater Quality and the Occurrence of Type 2 Diabetes in Rural Saskatchewan, Canada.

There is growing interest in the role of environmental exposures in the development of diabetes. Previous studies in rural Saskatchewan have raised concerns over drinking water contaminants, including arsenic, which has been identified as a possible risk factor for diabetes. Using administrative health and water-quality surveillance data from rural Saskatchewan, an ecological study design was used to investigate associations between concentrations of arsenic, water health standards and aesthetic objectives, and the incidence and prevalence of diabetes.

Scoping decades of dog evidence: a scoping review of dog bite-related sequelae.

Objectives: There has been considerable literature published focusing on various sequelae to dog bites over the last three decades. Much of the literature has focused on rabies, particularly canine rabies variant, which accounts for the majority of rabies deaths worldwide. This paper describes the complications, the pathogens, and other sequelae resulting from dog bites documented in the literature.

Exploring the Social Determinants of Animal Health.

An understanding of the One Health and EcoHealth concepts by students is dependent on medical pedagogy and medical pedagogy having similarities that allow a common discourse. Medical pedagogy includes a focus on the social, political, and economic forces that affect human health, while this discourse is largely absent from veterinary medical pedagogy. There is, however, a gradient in health that human and animal populations experience.

Ecological analysis of associations between groundwater quality and hypertension and cardiovascular disease in rural Saskatchewan, Canada using Bayesian hierarchical models and administrative health data.

Associations between groundwater quality and the prevalence of hypertension and ischemic heart disease were investigated in rural areas of the Canadian province of Saskatchewan. The partially ecological study was analyzed using Bayesian hierarchical models to account for spatial variability in risk. Exposure measures and health outcomes were estimated based on previously collected water quality surveillance data from public water supplies and private wells and administrative health data.