Experimental study of autologous free omental grafts.

The fate of intraabdominal free omental grafts was studied in 15 dogs and it was found that a connection between the hollow organ and the transplanted omentum was established only when the former had been injured. The larger, pigeon-egg-sized free fatty omental grafts became necrotic already from the fourth day onwards being replaced by an abscess-like structure, while in thin membrane-like grafts tissue necrosis due to the creeping in of connective tissue was less marked. In both cases, an intact, omentum is creeping over the graft from the adjacent region, i.

Partial experimental autotransplantation of the spleen.

The authors performed autotransplantation of the spleen in 45 dogs. They found that, the pieces of the transplanted spleen underwent necrosis, but new malphigian bodies formed on their surface. These latter took over the sepsis-inhibiting function of the spleen.

Restoration of skin defects with fibrin glue.

After experiments with absorbable bioplasts for substitution of skin defects, this time the absorbable fibrin glue named Tissucol was used by the authors. The experiments on 22 dogs unanimously proved that, beneath the layer of tissue adhesive, scarring occurred free of inflammation as compared to the controls where inflammatory phenomena were not excluded. The procedure has been found good and useful in high-degree burns and injuries up to their final management.

Experimental in situ renal hypothermia through the renal vein.

The authors worked out the technique of in situ renal hypothermia in dogs by direct cannulation of the renal vein or through the internal spermatic vein (ovarian vein). The technique is suggested for preventing the ischaemic impairment of the renal parenchyma in operations requiring compression of the renal artery for more than 20 minutes.

Auto-alloplastics closure of cervical esophageal fistulae.

The authors performed auto-alloplastic replacement with flap through a round incision, 20 to 22 in mm diameter, to elaborate a suitable method for closing the fistulae which may from during cervical esophageal operations. Of the animals three died due to other causes, the others could be evaluated, and, although the operated esophagus had not been relieved from inner pressure, after 24 hours the animals were capable of drinking, later eating. The auto-alloplastic flap was followed up by histological examination for six months.

Experimental partial splenectomy.

Partial splenectomy was performed on 25 dogs, removing the lower third of the spleen by a transverse incision. Stronger bleeding was ligated while parenchymatous haemorrhage was covered with Gelaspon plus Topostasin or Surgicel. The substances were sutured to the spleen with Dexon suture.

[Securing anastomosis of the large intestine with glued autologous tissue].

The colon was cut through across in 32 dogs without essential preparation. The recombination of the ends was carried out by a single-row stitched suture. This one was covered with an autologous strip of parietal peritoneum and fascia which was stuck by histoacryl-N-blue.

Intraductal pressure in experimental chronic pancreatitis and the magnitude of decompression after different types of operation.

The intraductal pressure parameters were studied in dogs with chronic pancreatitis. A ductal hypertension, similar to that in human chronic pancreatitis was demonstrated. Pressure fell after all different decompression procedures applied but it was most significant after longitudinal pancreatojejunostomy.

Comparative studies with extracorporeal hypothermal kidney perfusion.

Various solutions of extra- and intracellular, or non-electrolyte type supplemented with papaverine or lidocaine were administered by gravity perfusion to 60 dog kidneys. Cooling in the kidneys perfused with the solutions containing papaverine lowered the temperature of kidneys in significantly shorter time than in those containing lidocaine. The histological examinations revealed good perfusion with solutions containing papaverine; even the rim of the cortical substance has been freed of blood.

Sutures inserted into the gastric wall an experimental study.

Thirty gastrotomies were performed in 15 dogs using 2/0 atraumatic needles. Various kinds of threads were used: Dexon as an absorbable suture, Black Perlon and Black Braided Silk as nonabsorbable sutures. Ten suture rows were made with each material.

[Experimental surgery of the choledochus].

The authors carried out choledochal grafts in dogs with autologous parietal peritoneum and adjoining fascia of the musculus rectus abdominis. The patch was fixed with the tissue adhesive hystoacryl without suture. Additionally there was used an interior splinting with a polyethylene tube coated with paraffin.

Tissue reaction to Histoacryl Blue adhesive and histological investigation of its disappearance in various organs.

The effect of the adhesive Histoacryl Blue on the abdominal organs (liver, stomach, intestines, colon, kidneys, bladder, aorta inferior vena cava and skin wounds) of 15 dogs was investigated histologically. The adhesive was applied in thin or thick layers in various ways: applied on intact or injured surfaces, or introduced into the parenchymal organs. The animals were killed 1, 2, 4, 8 weeks and 3 and 6 months after application of the adhesive and the organs containing Histoacryl Blue were processed histologically.

Effect of testosterone on fracture healing in hypophysectomized rats.

The effect of hypophysectomy and hypophysectomy + testosterone treatment on fracture healing was investigated on 60 Wistar rats (30 day old). The aim of the experiment was to elucidate whether in callus formation testosterone acts directly on the cells (peripheral effect), or its effect is mediated through the hypophysis (central effect). Changes induced by hypophysectomy take place in the course of callus formation due primarily to the impairment of the enzyme system of the cells.

Effect of testosterone administration on the epiphyseal cartilage of hypophysectomized rats.

For the study of the mechanism of action of testosterone histological, carbohydrate-, and enzyme histochemical investigations were carried out on the epiphyseal cartilage of (1) hypophysectomized rats treated with testosterone [(THX group); (2)] hypophysectomized rats without hormonal treatment (HX group), and (3) intact, untreated control rats. The results were compared with the data obtained in a previous experiment in which intact rats were treated with testosterone (T group). The experiments showed that testosterone exerts a peripheral direct effect on the enzyme system of the epiphyseal cartilage cells by changing their metabolism in the direction of early ageing.