High-gain far-detuned nonlinear frequency conversion in a few-mode graded-index optical fiber.

We present theoretical and experimental evidence of high-gain far-detuned nonlinear frequency conversion, extending towards both the visible and the mid-infrared, in a few-mode graded-index silica fiber pumped at 1.064  , and more specifically achieving gains of hundreds of dB per meter below 0.65  and beyond 3.

Multiple intermodal-vectorial four-wave mixing bands generated by selective excitation of orthogonally polarized LP and LP modes in a birefringent fiber.

This study investigates the nonlinear frequency conversions between the six polarization modes of a two-mode birefringent fiber. The aim is to demonstrate that the selective excitation of different combinations of linearly polarized spatial modes at the pump wavelength initiates distinct intermodal-vectorial four-wave mixing processes. In particular, this study shows that exciting two orthogonally polarized LP and LP modes can lead to the simultaneous generation of up to three pairs of different spatial modes of orthogonal polarizations at different wavelengths.

All-normal dispersion supercontinuum vs frequency-shifted solitons pumped at 1560 nm as seed sources for thulium-doped fiber amplifiers.

We present a direct comparison between two types of femtosecond 2 µm sources used for seeding of an ultrafast thulium-doped fiber amplifier based on all-normal dispersion supercontinuum and soliton self-frequency shift. Both nonlinear effects were generated in microstructured silica fibers, pumped with low-power femtosecond pulses at 1.56 µm originating from an erbium-doped fiber laser.

Temporal fine structure of all-normal dispersion fiber supercontinuum pulses caused by non-ideal pump pulse shapes.

We experimentally investigate the spectro-temporal characteristics of coherent supercontinuum (SC) pulses generated in several implementations of silica and soft-glass all-normal dispersion (ANDi) photonic crystal fibers optimized for pumping with Erbium (Er):fiber femtosecond laser technology. We characterize the resulting SC using time-domain ptychography, which is especially suitable for the measurement of complex, spectrally broadband ultrashort pulses. The measurements of the ANDi SC pulses reveal intricate pulse shapes, considerable temporal fine structure, and oscillations on time scales of < 25 femtoseconds, which differ from the smoothness and simplicity of temporal profiles obtained in numerical simulations and observed in previous experiments.

Hybrid System Coupling Moving Bed Bioreactor with UV/O Oxidation and Membrane Separation Units for Treatment of Industrial Laundry Wastewater.

This paper describes the investigations on the possibilities of treatment of wastewater generated in an industrial laundry with application of a combined biological-photooxidation- membrane system aimed at water recycle and reuse. The two treatment schemes were compared: 1) scheme A consisting of a treatment in a moving bed biological reactor (MBBR) followed by microfiltration (MF) and nanofiltration (NF), and 2) scheme B comprising MBBR followed by oxidation by photolysis enhanced with in situ generated O (UV/O) after which MF and NF were applied. The removal efficiency in MBBR reached 95-97% for the biochemical oxygen demand; 90-93% for the chemical oxygen demand and 89-99% for an anionic and a nonionic surfactants.

Two SnRK2-Interacting Calcium Sensor Isoforms Negatively Regulate SnRK2 Activity by Different Mechanisms.

SNF1-related protein kinases 2 (SnRK2s) are key signaling elements regulating abscisic acid-dependent plant development and responses to environmental stresses. Our previous data showed that the SnRK2-interacting Calcium Sensor (SCS) inhibits SnRK2 activity. Use of alternative transcription start sites located within the Arabidopsis () gene results in two in-frame transcripts and subsequently two proteins, that differ only by the sequence position of the N terminus.

Compact all-fiber source of coherent linearly polarized octave-spanning supercontinuum based on normal dispersion silica fiber.

We report the generation of coherent octave-spanning supercontinuum in an all-fiber system, without any free-space optical components. The setup uses the femtosecond fiber laser as a pump and an all-normal dispersion microstructured fiber as a medium for supercontinuum generation. The generated spectrum is characterized both experimentally and numerically and shows a broad bandwidth (1.

Experimental Analysis of Bragg Reflection Peak Splitting in Gratings Fabricated Using a Multiple Order Phase Mask.

We performed an experimental analysis of the effect of phase mask alignment on the Bragg grating reflection spectra around the wavelength of = 1560 nm fabricated in polymer optical fiber by using a multiple order phase mask. We monitored the evolution of the reflection spectra for different values of the angle ϕ by describing the tilt between the phase mask and the fiber. We observed that the peak at is split into five separate peaks for the nonzero tilt and that separation of the peaks increases linearly with ϕ.

Influence of attenuation on self-organized second-harmonic generation in a germanium-doped microstructured silica fiber.

Observation of the efficient second-harmonic generation (SHG) in silica fibers drew a lot of interest, as second-order processes in centrosymmetric materials should not occur. In this Letter, we extended a theoretical model of self-organized SHG to include an attenuation and investigated the influence of fiber loss on the self-organized SHG process. We performed calculations of energy conversion efficiency for SHG in microstructured optical fibers.

The application of moving bed bio-reactor (MBBR) in commercial laundry wastewater treatment.

Large, laboratory scale biological treatment tests of real industrial wastewater, generated in a large industrial laundry facility, was conducted from October 2014 to January 2015. This research sought to develop laundry wastewater treatment technology which included tests of a two-stage Moving Bed Bio Reactor (MBBR); this had two reactors, was filled with carriers Kaldnes K5 (specific area - 800 m/m) and were realized in aerobic condition. Operating on site, in the laundry, reactors were fed actual wastewater from the laundry retention tank.

Polarized all-normal dispersion supercontinuum reaching 2.5 µm generated in a birefringent microstructured silica fiber.

We demonstrate a polarized all-normal dispersion supercontinuum generated in a birefringent silica microstructured fiber spanning beyond 2.5 µm. To our knowledge, this is the spectra reaching the furthest in mid-infrared ever generated in normal dispersion silica fibers.

Structural Dynamics of the GW182 Silencing Domain Including its RNA Recognition motif (RRM) Revealed by Hydrogen-Deuterium Exchange Mass Spectrometry.

The human GW182 protein plays an essential role in micro(mi)RNA-dependent gene silencing. miRNA silencing is mediated, in part, by a GW182 C-terminal region called the silencing domain, which interacts with the poly(A) binding protein and the CCR4-NOT deadenylase complex to repress protein synthesis. Structural studies of this GW182 fragment are challenging due to its predicted intrinsically disordered character, except for its RRM domain.

Tailoring supercontinuum generation beyond 2 μm in step-index tellurite fibers.

We report numerical and experimental demonstrations of flexible group-velocity dispersion regimes in step-index tellurite fibers by fine control of the fiber core diameter. Our simple fiber design allowed us to explore various nonlinear propagation regimes beyond 2 μm, which involved careful control of four-wave mixing processes. Combined with the recent development of 2 μm fiber lasers, we present an easy way to tailor supercontinuum generation and related coherence features in the high-demand 1.

Coherent supercontinuum generation up to 2.2 µm in an all-normal dispersion microstructured silica fiber.

For the first time to our knowledge, we demonstrate a coherent supercontinuum in silica fibers reaching 2.2 µm in a long wavelength range. The process of supercontinuum generation was studied experimentally and numerically in two microstructured fibers with a germanium doped core, having flat all-normal chromatic dispersion optimized for pumping at 1.

A system coupling hybrid biological method with UV/O3 oxidation and membrane separation for treatment and reuse of industrial laundry wastewater.

The possibilities of application of a three-step system combining hybrid biological treatment followed by advanced UV/O3 oxidation with in situ generated O3 and membrane separation (ultrafiltration (UF) and nanofiltration (NF)) to treat and reuse the wastewater from an industrial laundry are presented. By the application of a hybrid moving bed biofilm reactor (HMBBR), the total organic carbon concentration was reduced for about 90 %. However, since the HMBBR effluent still contained organic contaminants as well as high concentrations of inorganic ions and exhibited significant turbidity (8.

Mapping the Interaction Network of Key Proteins Involved in Histone mRNA Generation: A Hydrogen/Deuterium Exchange Study.

Histone pre-mRNAs are cleaved at the 3' end by a complex that contains U7 snRNP, the FLICE-associated huge protein (FLASH) and histone pre-mRNA cleavage complex (HCC) consisting of several polyadenylation factors. Within the complex, the N terminus of FLASH interacts with the N terminus of the U7 snRNP protein Lsm11, and together they recruit the HCC. FLASH through its distant C terminus independently interacts with the C-terminal SANT/Myb-like domain of nuclear protein, ataxia-telangiectasia locus (NPAT), a transcriptional co-activator required for expression of histone genes in S phase.

Analysis of distinct molecular assembly complexes of keratin K8 and K18 by hydrogen-deuterium exchange.

Keratins are intermediate filament (IF) proteins that form complex filament systems in epithelial cells, thus serving as scaffolding elements and mechanical stress absorbers. The building blocks of keratin IFs are parallel coiled-coil dimers of two distinct sequence-related proteins distinguished as type I and type II keratins. To gain more insight into their structural dynamics, we resorted to hydrogen-deuterium exchange mass spectrometry of keratins K8 and K18, which are characteristic for simple epithelial cells.

New Insights into Active Site Conformation Dynamics of E. coli PNP Revealed by Combined H/D Exchange Approach and Molecular Dynamics Simulations.

The biologically active form of purine nucleoside phosphorylase (PNP) from Escherichia coli (EC 2.4.2.

Birefringent optical fiber with dispersive orientation of polarization axes.

We demonstrate for the first time in optical fibers the "dispersion of the axes" phenomenon, which relies upon spectral dependence of polarization planes of the guided modes. This feature was achieved in a specially designed side-hole fiber by combining linear birefringence induced by a pair of holes located in the cladding and the elliptical core tilted with respect to the symmetry plane of the holes. We performed numerical simulations of this design properties.

Liquid crystal hyperbolic metamaterial for wide-angle negative-positive refraction and reflection.

We show that nanosphere dispersed liquid crystal (NDLC) metamaterial can be characterized in near IR spectral region as an indefinite medium whose real parts of effective ordinary and extraordinary permittivities are opposite in signs. Based on this fact we designed an electro-optic effect: an external electric-field-driven switch between normal refraction, negative refraction, and reflection of TM incident electromagnetic wave from the boundary vacuum/NDLC. A detailed analysis of its functionality is given based on effective medium theory combined with a study of negative refraction in anisotropic metamaterials and finite elements simulations.

Patterns of structural dynamics in RACK1 protein retained throughout evolution: a hydrogen-deuterium exchange study of three orthologs.

RACK1 is a member of the WD repeat family of proteins and is involved in multiple fundamental cellular processes. An intriguing feature of RACK1 is its ability to interact with at least 80 different protein partners. Thus, the structural features enabling such interactomic flexibility are of great interest.

Nonlinear frequency conversion in a birefringent microstructured fiber tuned by externally applied hydrostatic pressure.

We studied vector frequency conversion in externally tuned microstructured fibers for applications as a novel, nonlinear fiber-optic sensor. We investigated both experimentally and numerically a possibility of shifting vector and scalar modulation instability gain bands by pressure-induced changes in the linear properties of a microstructured fiber. Our results show that polarization-dependent vector nonlinear processes sensitive to variation of fiber group velocity difference (group birefringence) exhibit a clear advantage for pressure-sensing applications compared with scalar nonlinear processes only sensitive to group velocity dispersion changes.

Origin of Bragg reflection peaks splitting in gratings fabricated using a multiple order phase mask.

We have studied the effect of waveguide alignment on the reflection spectrum of Bragg gratings fabricated using a multiple order phase mask. We have demonstrated that splitting of certain Bragg peaks observed in earlier experiments reported in literature is caused by formation of the gratings with different periodicities in the waveguide tilted with respect to the phase mask plane due to the interference of non-symmetrical diffraction orders. Analytical expressions for spectral separation of the split peaks have been derived and verified against the experimental data recently presented in literature.