Can some food/medicinal plants directly affect porcine ovarian granulosa cells and mitigate the toxic effect of toluene?

The action of buckwheat, rooibos and vitex on healthy female reproductive systems, as well as their ability to mitigate the reproductive toxicity of environmental contaminant toluene have not yet been examined. We analysed the influence of toluene (0, 10, 100 or 1000 ng/mL) with and without these plant extracts (10 μg/mL) on cultured porcine ovarian granulosa cells. Cell viability, proliferation (PCNA accumulation), apoptosis (accumulation of bax) and release of progesterone (P) and oestradiol (E) were measured.

Ghrelin and obestatin can promote human ovarian granulosa cell functions and FSH effects.

The aim of the present in-vitro experiments was to examine the direct influence of ghrelin and obestatin on viability, proliferation and progesterone release by human ovarian granulosa cells and their response to FSH administration. Human granulosa cells were cultured in presence of ghrelin or obestatin (both at 0, 1, 10 or 100 ng/ml) alone or in the presence of FSH (10 ng/ml). Cell viability, accumulation of proliferation markers PCNA and cyclin B1 and release of progesterone were analyzed by Trypan blue extrusion test, quantitative immunocytochemistry and ELISA.

Can flaxseed, chia or puncture vine affect mare ovarian cell functions and prevent the toxic effect of the environmental contaminant toluene?

The aim of this in vitro study was to examine the potential effect of functional food plant extracts, namely, extracts of flaxseed (Linum usitatissimum L.), chia (Salvia hispanica) and puncture vine (Tribulus terrestris L.), on basic mare ovarian cell functions and their response to the environmental contaminant toluene.

Three medicinal plants affecting human ovarian cell viability, hormone release, and response to environmental contaminant toluene.

The present study examined the effect of medicinal plants - ginkgo, tribulus (puncture vine), and yucca - on ovarian functions and their response to the toxic influence of toluene. Therefore, we analyzed the effect of toluene with and without these plant extracts on cultured human ovarian granulosa cells. Cell viability and the release of progesterone, insulin-like growth factor I (IGF I), oxytocin, and prostaglandin F (PGF) were analyzed using the trypan blue test, enzyme immunoassay, and enzyme-linked immunosorbent assay, respectively.

Chia (Salvia hispanica L.) can directly suppress basic ovarian cell functions in two farm animal species and protect ovarian cells from the proliferation-stimulating influence of xylene.

The influence of the functional food plant chia (Salvia hispanica L.) on reproduction functions and its ability to prevent the negative effects of environmental contaminants has not yet been studied. Our study aimed to examine the effect of chia seed extract alone and in combination with xylene on the markers of proliferation, apoptosis and hormones release by cultured bovine and porcine ovarian granulosa cells.

Ginkgo, fennel, and flaxseed can affect hormone release by porcine ovarian cells and modulate the effect of toluene.

Experimental studies have documented the toxic effects of toluene on the mammalian female reproductive processes. The aim of this in vitro study was to examine the potential of functional food plant extracts, namely, of ginkgo, fennel, and flaxseed, in modifying the toluene-induced effects on ovarian hormone release. Porcine granulosa cells were incubated with ginkgo, fennel, or flaxseed extracts (0, 1, 10, or 100 µg/mL) and/or toluene (10 µg/mL).

Quercetin can affect porcine ovarian cell functions and to mitigate some of the effects of the environmental contaminant toluene.

The present in vitro experiments aimed to examine the effects of the plant polyphenol quercetin and the environmental contaminant toluene on basic ovarian cell functions, including the ability of quercetin to be a natural protector against the adverse effects of toluene. The influence of toluene, quercetin, and their combination on proliferation (accumulation of PCNA), apoptosis (accumulation of bax) and release of progesterone, testosterone and insulin-like growth factor I (IGFI) by cultured porcine ovarian granulosa cells was investigated. Toluene stimulated cell proliferation and inhibited progesterone, IGF-I and testosterone release but did not affect apoptosis.

: Direct action of leptin, obestatin and ginkgo on hormone release by luteinised human ovarian granulosa cells.

Context: The role of metabolic hormones, medicinal plants and their interrelationships in the control of human reproductive processes are poorly understood.

Aims: To examine how leptin, obestatin and ginkgo (Ginkgo biloba L.) affect human ovarian hormone release.

Maximum likelihood method of estimating the conflict-crash relationship.

The fundamental matters of how traffic conflicts are connected to crashes and how to estimate this connection with traffic conflict data is an active subject of research and refinements. There are still open questions about traffic events that can be analytically extrapolated to related crashes, and how to efficiently estimate the probability of crash associated with such events to enable conversion of observed events to the corresponding expected number of crashes. There are two important uses of a working estimation method: (1) rapid assessment of safety at specific roads locations and evaluation of countermeasures by safety engineers, (2) modeling of safety effects by analysts based on relatively short observations at multiple locations or at limited number of locations but during extended periods.

Direct action of leptin, obestatin and ginkgo on hormone release by luteinised human ovarian granulosa cells.

Context: The role of metabolic hormones, medicinal plants and their interrelationships in the control of human reproductive processes are poorly understood.

Aims: To examine how leptin, obestatin and ginkgo (Ginkgo biloba L.) affect human ovarian hormone release.

Tribulus terrestris can suppress the adverse effect of toluene on bovine and equine ovarian granulosa cells.

Influence of oil-related product toluene and herbal remedy puncturevine Tribulus terrestris L. (TT) on female reproduction is known. Yet, mechanisms of their action on ovaries in different species and potential protective effect of TT against adverse toluene action remain to be established.

Potential protective effect of puncturevine (Tribulus terrestris, L.) against xylene toxicity on bovine ovarian cell functions.

The action of the medicinal plant Tribulus terrestris (TT) on bovine ovarian cell functions, as well as the protective potential of TT against xylene (X) action, remain unknown. The aim of the present in vitro study was to elucidate the influence of TT, X and their combination on basic bovine ovarian cell functions. For this purpose, we examined the effect of TT (at doses of 0, 1, 10, and 100 ng/mL), X (at 20 ?g/mL) and the combination of TT + X (at these doses) on proliferation, apoptosis and hormone release by cultured bovine ovarian granulosa cells.

Towards human-like speed control in autonomous vehicles: A mountainous freeway case.

A driving strategy for autonomous vehicles (AVs) that is consistent with human behavior while demonstrating superior performance seems to have a good chance to be accepted by early AV users and be successful in the long run. Most of the past research focused on motion strategies affected by the presence of other vehicles. On the other hand, AV not constrained by other vehicles must select a safe and comfortable speed that is perceived as such by its occupants.

Feasibility study of highway alignment design controls for autonomous vehicles.

In recent years, the development and testing of autonomous driving technology have become widespread around the world. However, due to differences in perception abilities between autonomous vehicles and human drivers, the current geometric design controls for highway alignments, designed for the human driver, may not be applicable to the autonomous vehicle (AV). Few studies, however, have systematically investigated the design controls for autonomous vehicles, though we face full driving automation in the next few decades.

A unifying view on traffic conflicts and their connection with crashes.

After decades of research on traffic conflicts and other crash surrogate events, defining these events and conclusively connecting them with crashes continue to be the most important tasks. This paper aims to help establish a consensus on these two fundamental matters by discussing the underlying concepts by which they can be connected in a consistent construct justified with theory and empirical evidence. The importance of insight into a safety-relevant event beyond what is externally observable is emphasized by considering two distributions of crash nearness: (1) values observed by external observers and (2) driver-preferred values that are usually unobservable.

Transferability analysis of the freeway continuous speed model.

Operating speed is often used to evaluate consistency in road geometric design. In the China, the Specifications for Highway Safety Audit includes a spot-based speed model that predicts operating speed by dividing the road into homogeneous segments and observing the speeds at sparsely spaced spots. This paper presents a continuous speed model as a more representative alternative for roads with complex alignments, and can be applied to tunnel sections as one general model.

Validity of failure-caused traffic conflicts as surrogates of rear-end collisions in naturalistic driving studies.

Although the frequency and severity of crashes are direct measures of road safety, crash data are typically of limited quality and they require long data collection periods to produce conclusive results. Surrogates of crashes that would allow a quick and accurate estimation of safety have been an active topic for years. Among multiple alternatives, traffic conflicts have been established as a promising surrogate measure.

Bee pollens originating from different species have unique effects on ovarian cell functions.

Context: The species-specific differences and mechanisms of action of bee pollen on reproduction have not been well studied.

Objective: We compared the effects of bee pollen extracts from different plants on ovarian cell functions.

Materials And Methods: We compared the effects of pollens from black alder, dandelion, maize, rapeseed, and willow at 0, 0.

Plant polyphenols can directly affect ovarian cell functions and modify toluene effects.

The influence of toluene alone and in combination with plant polyphenols apigenin, daidzein or rutin on viability, proliferation (proliferating cell nuclear antigen accumulation), apoptosis (Bax accumulation) and release of progesterone (P), testosterone (T) and estradiol (E) in cultured porcine ovarian granulosa cells was evaluated. Toluene reduced ovarian cell viability, proliferation and E release; it promoted P release, demonstrating no effect on apoptosis or T output. Apigenin alone failed to affect cell viability, proliferation, apoptosis and P and T release, but stimulated E release, promoting the inhibitory action of toluene on proliferation, preventing and even reversing the stimulatory effect of toluene on apoptosis and P.

Buckwheat, rooibos, and vitex extracts can mitigate adverse effects of xylene on ovarian cells in vitro.

This study examines whether selected functional food and medicinal plants can mitigate the adverse effects of xylene on ovarian cells. The influences of xylene (0, 10, 100, or 1000 ng/mL), buckwheat (Fagopyrum esculentum), rooibos (Aspalathus linearis), vitex (Vitex agnus-castus), extracts (10 μg/mL each), and a combination of xylene with these plant additives on cultured porcine ovarian granulosa cells are compared. Cell viability, proliferation (PCNA accumulation), apoptosis (accumulation of bax), and release of progesterone (P4) and estradiol (E2) were analyzed by the trypan blue tests, quantitative immunocytochemistry, and enzyme-linked immunosorbent assays, respectively.

Abatement of the Stimulatory Effect of Copper Nanoparticles Supported on Titania on Ovarian Cell Functions by Some Plants and Phytochemicals.

The application of nanoparticles has experienced a vertiginous growth, but their interaction with food and medicinal plants in organisms, especially in the control of reproduction, remains unresolved. We examined the influence of copper nanoparticles supported on titania (CuNPs/TiO), plant extracts (buckwheat () and vitex ()), phytochemicals (rutin and apigenin), and their combination with CuNPs/TiO on ovarian cell functions, using cultured porcine ovarian granulosa cells. Cell viability, proliferation (PCNA accumulation), apoptosis (accumulation of bax), and hormones release (progesterone, testosterone, and 17-estradiol) were analyzed by the Trypan blue test, quantitative immunocytochemistry, and ELISA, respectively.

Effects of benzene on gilts ovarian cell functions alone and in combination with buckwheat, rooibos, and vitex.

We aimed to examine the influence of benzene and of three dimethyl sulfoxide (DMSO) plant extracts-buckwheat (Fagopyrum Esculentum), rooibos (Aspalathus linearis), and vitex, (Vitex Agnus-Castus), and the combination of benzene with these three plant extracts on basic ovarian cell functions. Specifically, the study investigated the influence of benzene (0, 10, 100, or 1000 ng/mL) with and without these three plant additives on porcine ovarian granulosa cells cultured during 2 days with and without these additives. Cell viability, proliferation (accumulation of proliferating cell nuclear antigen, PCNA), apoptosis (accumulation of Bcl-2-associated X protein , bax), and the release of progesterone (P) and estradiol (E) were analyzed by the Trypan blue test, quantitative immunocytochemistry, and enzyme-linked immunosorbent assay, respectively.

Plant isoflavones can prevent adverse effects of benzene on porcine ovarian activity: an in vitro study.

We evaluated the influence of the oil-related environmental contaminant benzene (0, 10, 100, or 1000 ng/mL) alone and in combination with apigenin, daidzein, or rutin (10 μg/mL each) on viability; proliferation (accumulation of proliferating cell nuclear antigen); apoptosis (accumulation of Bax); and release of progesterone (P), testosterone (T), and estradiol (E) in cultured porcine ovarian granulosa cells. Cell viability; proliferation; apoptosis; and release of P, T, and E have been analyzed by the trypan blue test, quantitative immunocytochemistry, and ELISA, respectively. Benzene did not affect apoptosis, but reduced ovarian cell viability and P and E release, and promoted proliferation and T output.

Analyzing road near departures as failure-caused events.

Surrogate measures of safety attract revived interest thanks to the advancements in traffic observations techniques and the growing need for rapid safety evaluation. A new method of safety analysis based on failure-caused traffic conflicts and the Lomax distribution was recently proposed to estimate crash frequency more efficiently than with crash data. This paper has two objectives: (1) demonstrate the method applicability to near-departure data collected in a driving simulator, and (2) provide initial evidence of the method validity.

The inhibitory influence of toluene on mare ovarian granulosa cells can be prevented by fennel.

The influence of environmental contaminant toluene and of plant fennel (Foeniculum vulgare Mill.) on reproduction are reported, but the mechanisms of their action and the protective effect of fennel on contaminant influence remain to be elucidated. In this study, we hypothesized that toluene and fennel directly affects basic ovarian cell functions, and that fennel can be used as an appropriate natural protective agent against the potential adverse effects of toluene.