The Biogenic Synthesis of Bimetallic Ag/ZnO Nanoparticles: A Multifunctional Approach for Methyl Violet Photocatalytic Degradation and the Assessment of Antibacterial, Antioxidant, and Cytotoxicity Properties.

In this study, bimetallic nanoparticles (NPs) of silver (Ag) and zinc oxide (ZnO) were synthesized using leaf extract for the first time. Monometallic NPs were also obtained for comparison. The characterization of the prepared NPs was carried out using various techniques, including UV-Visible spectroscopy (UV-Vis), scanning electron microscopy (SEM), and X-ray diffraction (XRD).

Coupling hydrogeochemistry and stable isotopes (δH, δO and δC) to identify factors affecting arsenic enrichment of surface water and groundwater in Precambrian sedimentary rocks, eastern salt range, Punjab, Pakistan.

The study area is a part of the Salt Range, where water quality is being deteriorated by natural and anthropogenic sources. This research integrates water quality assessment, arsenic enrichment, hydrogeochemical processes, groundwater recharge and carbon sources in aquifer. Total dissolved solid (TDS) contents in springs water, lake water and groundwater are in range of 681-847 mg/L, 2460-5051 mg/L and 513-7491 mg/L, respectively.

Isolation, identification, and pathogenicity analysis of newly emerging gosling astrovirus in South China.

Goose astroviruses (GoAstV) cause fatal gout and decrease product performance in the waterfowl industry across the world. Since no effective vaccines are available, studies on the epidemiology of the virus are necessary for vaccine development. In this study, we collected 94 gout samples from goose farms in the Guangdong Province of South China.

A novel synthetic derivative of biaryl guanidine as a potential BACE1 inhibitor, to treat Alzheimer's disease: In-silico, in-vitro and in-vivo evaluation.

BACE1 enzyme has been known a potential target involved in Alzheimer's disease (AD). Present research was focused on the principles of virtually screening, chemical synthesis and protease inhibitory effect of BACE1 enzyme via biaryl guanidine derivatives. In-silico based paradigm (ligand binding interaction within active domain of BACE 1 enzyme i.

Morphometric parameters and food preference in relation to sex and reference hematological values for from Pakistan.

Objective: The study was conducted to investigate the gut content and record morphometric and hematological parameters in the common hoopoe ().

Materials And Methods: Twenty samples of healthy birds (10 from each sex) were collected from different locations in Okara District, Punjab, Pakistan, from September 2020 to March 2021. Birds were captured live for blood samples and morphometric and gut analyses.

Post-traumatic Renal Arteriovenous Malformation Discovered after 2 Years of Blunt Trauma: Diagnosis and Management.

Blunt trauma to the kidney is usually minor and self-limiting. In some cases, the patient may present with intractable hematuria or persistent flank pain and discomfort, which necessitates aggressive management. It is very rare and unusual to find a renal arterio-venous malformation following blunt trauma.

Biochemical characterization, cytotoxic, antimutagenic, anticancer and molecular docking studies on .

In this study bioassay-guided screening of Tecomella undulate was performed for its cytotoxic, antimutagenic and anticancer potential. The ariel parts were extracted on a polarity basis (methanol, dichloromethane and hexane). The in vivo toxicity was assessed on Caenorhabditis elegans, and its locomotion was affected by Tecomella undulata hexane (TUAH) the most.

Sequestration of crystal violet dye from wastewater using low-cost coconut husk as a potential adsorbent.

The current study explores the effectiveness of coconut husk for crystal violet dye sequestration employing a batch experimental setup. Characterization of adsorbent was carried out via FTIR, and SEM techniques and results confirmed the involvement of -OMe, -COC- and hydroxyl functional groups in dye uptake, and the rough, porous nature of adsorbent and after adsorption dye molecules colonized these holes resulting in dye exclusion. Effects of various adsorption parameters such as pH, adsorbent dose, contact time, initial dye concentration, and temperature of solution were studied.

Epidemiological studies of sleep disorder in educational community of Pakistani population, its major risk factors and associated diseases.

Sleep is one of the most important functions of the life. The disturbance in sleep or quality of sleep leads to several dysfunctions of the human body. This study aimed to investigate the prevalence of sleep disorders, their possible risk factors and their association with other health problems.

In vitro antimutagenic, cytotoxic and anticancer potential of Fagonia indica phytochemicals.

Cancer is one of the most diagnosed and life threatening disease throughout the world. Nevertheless present day clinical management for cancers are surgery, radiations which are insufficient to contain the disease burden. In the past two decades, more than half of chemotherapeutic drugs developed are either directly or indirectly dependent on medicinal base phytocompounds or their derivative.

Anti-hepatitis C virus and synergistic potential of Syzgium cumine a bioassay guided screening in liver-infected hepatocytes.

Hepatitis C virus is still a global challenge affecting millions of carriers worldwide with the more devastating situation in developing countries. Present-day clinical manifestations are insufficient to tackle the increasing disease burden unaffordable cost, viral resistance and adverse effects of treatment. In this research, indigenous medicinal plants from Pakistan tested in bioassay guided manner on Huh-7 cell lines for their antiviral effect, synergism of purified fraction with interferon FDA approved drug regime, as the receptor for developing transfection model.

In silico bimolecular characterization of anticancer phytochemicals from Fagonia indica.

The in silico molecular dynamics and structure-based site-specific drug design of indigenous plant biomolecules and selected proteins have remarkable potential for cancer therapy. A set of five proteins included for this research were epidermal growth factor protein (PDB ID; 1M17), crystal structure of mutated EGFR kinase (PDB ID; 2EB3), crystal structure of Bcl-xl (PDB ID; 2YXJ), apoptosis regulator protein MCL-1 BH3 (PDB ID; 3MK8) and apoptosis proteins (PDB ID; 5C3H). The present study on in silico investigation of fifteen indigenous medicinal plants were selected there one hundred thirty four ligands available literature were docked against five proteins involved in carcinogenesis.

Seroprevalence and pathological studies of Salmonella infection in commercial white layer birds.

The present study was conducted to determine the seroprevalence and pathology of Salmonella infection in white commercial layer birds of District Faisalabad during June 2018 and June 2020. The current study aimed to determine the isolation, identification of Salmonella gallinarum (S. gallinarum), its cultural prevalence, antimicrobial resistance, molecular characterization, and pathological lesions produced in different organs of commercial layer birds.

Long-term exposure to the fluoride blocks the development of chondrocytes in the ducks: The molecular mechanism of fluoride regulating autophagy and apoptosis.

Long-term exposure to excessive fluoride causes chronic damage in the body tissues and could lead to skeletal and dental fluorosis. Cartilage damage caused by excessive fluoride intake has gained wide attention, but how fluoride accumulation blocks the development of chondrocytes is still unclear. Here, we report a negative correlation between the length and growth plate width after NaF treatments via apoptosis and autophagy, with shrinkage of cells, nuclear retraction, dissolution of chondrocytes.

Green synthesis of Fe nanoparticles by using Mangifera indica extract and its application in photo-catalytic degradation of dyes.

An exceedingly facile green approach that produces a reliable adsorbent based on a transition metal such as Iron (Fe) using Mangifera indica leaf extract at room temperature is described. A single pot method was used for synthesis with no capping agents, surfactants or other templates. The main intention of this study is to synthesize iron nanoparticles from leaf extract (Mangifera indica) and examine its degradation potential for photo-catalytic removal of dyes (Congo red and brilliant green) from wastewater.

Role of Ferrous Sulfate (FeSO) in Resistance to Cadmium Stress in Two Rice ( L.) Genotypes.

The impact of heavy metal, i.e., cadmium (Cd), on the growth, photosynthetic pigments, gas exchange characteristics, oxidative stress biomarkers, and antioxidants machinery (enzymatic and non-enzymatic antioxidants), ions uptake, organic acids exudation, and ultra-structure of membranous bounded organelles of two rice ( L.

Salinity enrichment, sources and its contribution to elevated groundwater arsenic and fluoride levels in Rachna Doab, Punjab Pakistan: Stable isotope (δH and δO) approach as an evidence.

The present study aimed at exploring the sources of salinity and the link it shares with the enrichment of As (arsenic) and F- (fluoride) in the groundwater of Rachna Doab. Total Dissolved Solids (TDS) were used as the measure of salinity to classify samples into three groups: TDS <1000 mg/L (freshwater), 1000-3000 mg/L (slightly saline) and 3000-10,000 mg/L (moderately saline). The stable isotope analysis (δH and δO relative to VSMOW) were used to explore the sources of salinity and a conceptual model, based on secondary data was used for comparing the current and past scenarios of groundwater salinization sources.

Ethylenediaminetetraacetic Acid (EDTA) Mitigates the Toxic Effect of Excessive Copper Concentrations on Growth, Gaseous Exchange and Chloroplast Ultrastructure of L. and Improves Copper Accumulation Capabilities.

Copper (Cu) is an important micronutrient for a plant's normal growth and development. However, excess amount of Cu in the soil causes many severe problems in plants-which ultimately affect crop productivity and yield. Moreover, excess of Cu contents causes oxidative damage in the plant tissues by generating excess of reactive oxygen species (ROS).

Synthesis, characterization and docking studies of amide ligands as anti-leishmanial agents.

Aim of this study was to synthesize new inhibitors on the basis of active site of aspartic protease enzyme and to evaluate their intended biological activity. A3D model of an enzyme was generated via homology modeling and series of novel amide ligands were synthesized by using a short high yield process, subsequently, analyzed in-silico and in-vitro anti-leishmanial activities. Characterization and identification was accomplished via NMR (H& C), infrared and mass spectroscopic techniques.

Pathological and clinical investigations of an outbreak of Blackleg disease due to C. chauvoei in cattle in Punjab, Pakistan.

Introduction: Clostridium chauvoei (C. chauvoei) is an anaerobic, histotoxic Gram-positive, bacterium causing fatal myonecrosis in livestock with high mortalities. The disease is common in dairy animals, but little is known about the pathophysiology of the disease in exotic (non-native) animals kept under local conditions in Pakistan.

Evaluation of different Pakistani medicinal plants for inhibitory potential against Echis carinatus induced Phospholipase A2 toxicity.

Medicinal plants of Pakistan are known for their curative properties against snake bite as rural people have been using natural herbs for such injuries for hundreds to thousands of years. People of rural areas of Pakistan are prone to snakebite, and on the whole death due to snakebite has been increasing worldwide. The objective of this study was to test the neutralizing potential of 17 Pakistani medicinal plant extracts against phospholipase A2 activity in Echis carinatus venom.