[Automation of hematologic studies in a clinical-diagnostic laboratory in a multi-profile hospital].

The authors share their experience gained with automation of hematologic investigations at a clinical diagnosis laboratory. A CDL information system, basing on personal computers, has been created, functioning in complex with hematologic autoanalyzers. The reports on the laboratory's activity are made automatically, as is the laboratory register and processing of applications for tests.

[Ecological approaches to the study of natural-focus diseases].

Changes in the epidemiology and epizootiology of diseases with natural foci due to anthropogenic influence on biosphere necessitate thorough studies of ecological specific features of agents, carriers and vertebral hosts reflecting their adaptation to various natural geographical and social conditions. The attention should be focussed on geographical variability of agents, changes in populations of arthropod carriers and their feeders, monitoring of natural territorial complexes and scientifically grounded prognosis for diseases with natural foci.

[Quantitative changes of the blood serum immunoglobulins in patients with diabetes mellitus types I and II and microangiopathy detected by morphologic study].

A study was made of the immunological mechanisms implicated in the evolution of diabetic microangiopathy. For this purpose in 270 patients with type I and II diabetes mellitus, the concentration of IgA, IgM and IgG was measured and compared with morphological alterations in skin biopsy specimens. The control group was made up of 30 normal persons (donors).

[Induction of SOS function in Escherichia coli after exposure to various types of chemical mutagens].

On the basis of the SOS-chromotest which was developed by the authors earlier, the minimum time required for expression of the SOS-response is shown to be 15 min, the maximum being recorded 2 h later. An assay of induction of the SOS-response to the mutagens which act on DNA via various mechanisms (e. g.

[Participation of the uvrD gene in the precise excision of the Tn9 transposon].

HfeTn5-(04,06) and IfeTn9 mutations increase efficiency of precise excision of Tn5, Tn10 and decrease that of Tn9. These mutations have been mapped in uvrD gene. In LFETn9 and UVRE502 mutants, the multicopy plasmid pEM61 carrying the cloned uvrD gene complements LFETn9- phenotype (Low Frequency Excision of Tn9).

[Characteristics of Escherichia coli K-12 mutants with altered effectiveness of excision of Tn5 and Tn9 transposons].

The properties of Escherichia coli K-12 mutans HFETn5, HFETn9 and LFETn9 have been studied. The majority of mutations were shown to have pleiotropic effect. Some of them increase cell sensitivity to UV light and mitomycin C and affect efficiency of homologous recombination in transduction and conjugation.

[Mutants of Escherichia coli K-12 with altered excision efficiency of transposons Tn5 and Tn9].

HFETn5, HFETn9 and LFETn9 mutants of Escherichia coli K-12 have been isolated. The frequency of Tn5 precise excision from the chromosomal lac operon is increased 3-660-fold in nine HFETn5 mutants. The majority of these mutations have no influence on the efficiency of precise excision of transposon Tn9, though hfeTn5-04 and hfeTn5-06 mutations decrease excision efficiency 2-13-fold.

[Cloning the genetic determinant of alpha-hemolysin and obtaining a series of insertional mutations in this determinant by the transposon Tn1000].

Functionally active genetic determinant of alpha-hemolysin was cloned. Hemolytic plasmid pHly195 was used as a donor of the determinant and pBR322 plasmid served as recipient. Cloning was done with a help of HindIII restriction endonuclease.

[Drainage of the uterine cavity in postpartum endometritis].

Analyzes the results of management of protracted torpid postpartum endometritis (10 cases) and severe septic endometritis following a cesarean section (9 cases), multiple-modality treatment of these patients including prolonged influent-aspiration drainage of the uterine cavity. Describes the drainage system, its fixation, and operation. Discusses a differentiated approach to infusion of the drugs directly into the uterine cavity in cases with the uterine inflammations.

[Laparoscopy in the diagnosis of acute appendicitis in children].

Based upon an analysis of results of 1210 operations and 160 laparoscopies in children for "acute appendicitis" the authors make a conclusion that about 20% of children were subjected to unnecessary appendectomies. Laparoscopy allows to make the correct diagnosis in the absolute majority of children with obscure clinical picture of acute appendicitis and to choose a rational medical treatment.

[Use of automation potential in intensive care units of a multi-profile hospital].

An example of an automated information system of a large general hospital has shown that using a sufficiently informative clinical data base (with a minimal set of parameters) on patients in intensive care departments, it is possible to provide information inquiries of diagnostic units and hospital administration without distracting staff-members on duty to report on the patients' state. The use of an automated bed turnover management system made it possible to reduce the time of patients' admission to hospital, to improve the planning of hospitalization and hospital transfers, to raise the efficacy of bed turnover, and to reduce unproductive labor of the hospital staff.