[Sialic acids and O-acetyl groups as markers of biological activity of microbial polysaccharides in plague and cholera agents].

Aim: To determine sialic acids and O-acetyl groups content in Yersinia pestis and Vibrio cholerae antigens in order to establish their association with biological activity.

Materials And Methods: The following antigens of Y. pestis EV NIIEG strain--capsular antigen (F1), major somatic antigen (MSA), lipopolysaccharide (LPS), Pla-protease, allergen pestin PP--as well as O-antigens (O-AG) of V.

[The effect of Yersinia pestis antigens and strains on the level of lymphocyte blast transformation].

Lymphocytic blast-cell transformation (LBCT) is shown to be related both to the genotype of a macroorganism and to the genetically determined expression of individual antigens by the plague microbe. The correlation of LBCT with the level of antiinfectious resistance suggests that this index is of high informative value and that it can be effectively used for testing the cellular immunity and the characteristically biological properties of the plague causative agent and its antigens.

[Comparative incidence of detection of specific antibodies to Yersinia pestis capsular antigen and lipopolysaccharide in humans immunized with pest vaccine].

Two to 8 years after vaccination, specific antibodies to capsular antigen (F1) and lipopolysaccharide (LPS) are found in the blood sera of but 10 to 20% of subjects 1-4 times epicutaneously immunized with live antipest vaccine. Only regular continuous (for at least 7-15 years) antipest vaccination leads to the production of antibodies to the main antigens of Yersinia pestis, detectable in remote periods in 75.9 +/- 4.

[Interleukin-1 induction by different antigens and strains of Yersinia pestis bacteria in immunized mice].

In this work the influence of Y. pestis antigens (capsular antigen, mouse toxin, lipopolysaccharide, pesticin), as well as the products of gene expression localized on three Y. pestis resident plasmids, have been studied.

[Effect of plague microbe fraction 1 protein-polysaccharide complex and pure protein on metabolic parameters of macrophages].

The investigation reveals different influence of the plague microbe's fraction 1 polysaccharide-protein complex and of it's purified protein on the 5'-nucleotidase activity and on the chemiluminescence response of peritoneal macrophages. Both of these metabolic indexes were found to be dependent on the dose of fraction 1 and duration of time till examination.

[Sensitivity to mouse toxin and level of macrophage 5-nucleotidase activity].

It is shown that immunomodulator of bacterial origin salmozan causes alternations of sensitivity to mouse toxin in mice CBA. A correlation exists between the sensitivity to mouse toxin and the level of 5-nucleotidase of peritoneal exudate macrophages.

[A comparative cytofluorimetric analysis of the blood leukocytes in guinea pigs exposed to the phospholipase D and antigens of the causative agent of plague].

The influence of Y. pestis phospholipase D on the physiological state of leukocytes in the blood of guinea pigs was studied in vivo by flow impulse fluorometry with the use of fluorochrome acridine orange. During the first hours of observation the intensity of leukocyte fluorescence increased due to a rise in the number of polymorphonuclear leukocytes and changes in the permeability of cell membranes.

[A morphofunctional analysis of the parietal exocrinocytes of the gastric glands proper after removal of the testes].

In the experiment performed in 52 white male rats by means of light electron microscopy methods, cytochemistry and biochemistry with a subsequent cytophoto- and morphometric analysis, it has been stated that bilateral removal of the testes does not inhibit essentially the differential process of the parietal exocrinocytes, but facilitates the development of certain dystrophic processes in them. The latter are demonstrated as decrease of secretory membranes balance in the cell, of quantitative density of mitochondria, of crysts area, of matrix vacuolization. Activity of oxidoreductase in Krebs cycle, of pentosophosphate shunt and glycolysis decreases.

[The autoselection of neustonic forms of bacteria].

Self-breeding of neuston forms of Methylobacterium sp., Pseudomonas putida BC-2, Alcaligenes paradoxus BC-1, Bacillus thuringiensis var. israilensis bacteria as well as of a mixed culture of methylotrophs is shown possible.

[The flotation characteristics of Bacillus cells and spores].

Variations in hydrophobicity of the surface of bacillary cells and their capacity to flotation in the process of batch cultivation have been studied. It is shown that hydrophobicity of the cell surface increases in the course of batch cultivation of Bacillus thuringiensis, B. licheniformis and B.

[The effect of different Yersinia pestis antigens on the cellular link in immunity].

Immunization with live plague vaccine has been shown to give no protection to thymectomized mice from subcutaneous challenge with Y. pestis virulent strain. Under the action of the vaccine or individual Y.

[The role of T-suppressors in the formation of plague immunity in mice].

Antiplague immunization of mice causes an increase in the number of T-suppressors in their thymus and spleen; this increase is especially pronounced after injection of a low-immunogenic dose of the vaccine strain. T-suppressors specific to Yersinia pestis antigens were detected in the thymus on day 3 after a single injection and on day 14 after two injections of the vaccine strain.

[Various physico-chemical properties of the Yersinia pestis capsular protein].

Some properties of the structure of Y. pestis capsular antigen macromolecules have been studied. The aminoacid composition of F1 protein, the aminoacid sequence of the N-terminal fragment of antigen polipeptide chain were determined.

[Characteristics of the polysaccharide-containing somatic antigens isolated from the K-1 strain of the plague microbe and its antibiotic-resistant variants].

Immunochemical analysis of 2 polysaccharide-containing structures of the lypopolysaccharide of the plague causative agent (main somatic antigen and lipopolysaccharide) isolated from K-1 strain and a number of its antibiotic resistant mutants was carried out. It was shown that development of resistance to streptomycin alone or its combination with monomycin did not cause detectable changes in the monosaccharide composition and serological properties of the cultures tested. More significant changes associated with development of complex resistance, i.