[Effect of conditions of monoclonal antibody adsorption on antigen-binding activity].

The dependence of the antigen-binding activity of immobilized antibodies on pH of a saturating buffer has been investigated. We analyzed 28 monoclonal antibodies (MCAs) produced by various hybridomas to three virus antigens, i.e.

[Use of pH-sensitive immobilized monoclonal antibodies for optimization of immunoenzyme sandwich technique of detection of HBsAg of hepatitis B virus].

Aim: To develop highly sensitive sandwich technique for identification of surface hepatitis B virus antigen (HBsAg) in serum and analyse of possible improvement of solid phase for immunoenzyme sandwich technique of HBsAg identification through variation of pH-dependent sorption of monoclonal antibodies on the surface of immune plates.

Materials And Methods: Calibration curves for identification of HBsAg in sandwich techniques using 36 possible binary combinations of monoclonal antibodies of our panel (including high affinity antibodies to HBsAg produced by 6 hybridomas) were compared. Immobilization of antibodies on solid phase (by passive sorption) was performed at different pH values (2.

[Formation of dose fields in local irradiation of cancer of the prostate].

The authors compare dose distributions in local irradiation of prostatic cancer using moving methods on distant gamma-units while taking and not taking into account individual structure of body section and bone structures. They propose problems of choice of optimum conditions of gamma-beam therapy in localized prostatic cancer and their solution. Optimum irradiation designs have been found preferable.

[Retroperitoneal injuries of the duodenum].

8 observations over a closed trauma of the retroperitoneal portion of the duodenum are presented; 3 patients out of them sustained combined traumas. Only in 2 cases the rupture of the peritoneal portion of the duodenum was suspected before operation. In 4 patients the diagnosis was established in time of laparotomy, in 2 cases the rupture was recognized at the repeated laparotomy.

[Injuries of the heart and pericardium].

The authors experience with surgical treatment of heart and pericardium injuries is summarized. A total of 38 patients were operated upon. The surgical aid consisted in simultaneous performance of the complex of reanimation measures, urgent thoracotomy and suturing of the heart wound.