A chromosome-scale genome assembly of the grape powdery mildew pathogen reveals its genomic architecture and previously unknown features of its biology.

is an obligate fungal pathogen that causes grape powdery mildew, globally the most important disease on grapevines. Previous attempts to obtain a quality genome assembly for this pathogen were hindered by its high repetitive DNA content. Here, chromatin conformation capture (Hi-C) with long-read PacBio sequencing was combined to obtain a chromosome-scale assembly and a high-quality annotation for isolate EnFRAME01.

A Rapid Glove-Based Inoculum Sampling Technique to Monitor in Commercial Vineyards.

Information on the presence and severity of grape powdery mildew (GPM), caused by , has long been used to guide management decisions. While recent advances in the available molecular diagnostic assays and particle samplers have made monitoring easier, there is still a need for more efficient field collection of . The use of vineyard worker gloves worn during canopy manipulation as a sampler (glove swab) of was compared with samples identified by visual assessment with subsequent molecular confirmation (leaf swabs) and airborne spore samples collected by rotating-arm impaction traps (impaction traps).

Development of a PNA-LNA-LAMP Assay to Detect an SNP Associated with QoI Resistance in .

The repetitive use of quinone outside inhibitor fungicides (QoIs, strobilurins; Fungicide Resistance Action Committee [FRAC] 11) to manage grape powdery mildew has led to development of resistance in . While several point mutations in the mitochondrial cytochrome gene are associated with resistance to QoI fungicides, the substitution of glycine to alanine at codon 143 (G143A) has been the only mutation observed in QoI-resistant field populations. Allele-specific detection methods such as digital droplet PCR and TaqMan probe-based assays can be used to detect the G143A mutation.

Identification of Putative SDHI Target Site Mutations in the SDHB, SDHC, and SDHD Subunits of the Grape Powdery Mildew Pathogen .

Succinate dehydrogenase inhibitors (SDHIs) are fungicides used in control of numerous fungal plant pathogens, including , the causal agent of grapevine powdery mildew (GPM). Here, the , , and genes of were screened for mutations that may be associated with SDHI resistance. GPM samples were collected from 2017 to 2020 from the U.

Allele-Specific Detection Methods for QoI Fungicide-Resistant in Vineyards.

Grapevine powdery mildew (GPM), caused by the fungus , is a constant threat to worldwide production of grape berries, requiring repeated use of fungicides for management. The frequent fungicide applications have resulted in resistance to commonly used quinone outside inhibitor (QoI) fungicides and the resistance is associated with single-nucleotide polymorphisms (SNPs) in the mitochondrial gene (). In this study, we attempted to detect the most common SNP causing a glycine to alanine substitution at amino acid position 143 (i.

Development of a quantitative loop-mediated isothermal amplification assay for the field detection of .

Plant pathogen detection systems have been useful tools to monitor inoculum presence and initiate management schedules. More recently, a loop-mediated isothermal amplification (LAMP) assay was successfully designed for field use in the grape powdery mildew pathosystem; however, false negatives or false positives were prevalent in grower-conducted assays due to the difficulty in perceiving the magnesium pyrophosphate precipitate at low DNA concentrations. A quantitative LAMP (qLAMP) assay using a fluorescence resonance energy transfer-based probe was assessed by grape growers in the Willamette Valley of Oregon.