Upconverting Luminescent MOF for Highly Sensitive Dual-Mode Recognition of Synthetic Dyes.

Lanthanide-based luminescent materials hold promise in sensing applications due to their distinct optical properties. Though advancements in lanthanide-based metal-organic frameworks (MOFs) have enhanced downshifting luminescence, achieving upconversion remains challenging. In this effort, we prepared upconverting ytterbium-doped europium MOFs (%Yb-EuMOFs; = 10, 20, and 30) via the solvothermal method using 2,6-naphthalenedicarboxylic acid (NDC) as an organic linker.

Turn-on detection of Al ions using quinoline-based tripodal probe: mechanistic investigation and live cell imaging applications.

In this study, an easily synthesizable Schiff base probe TQSB having a quinoline fluorophore is demonstrated as a fluorescent and colorimetric turn-on sensor for Al ions in a semi-aqueous medium (CHCN/water; 4 : 1; v/v). Absorption, emission and colorimetric studies clearly indicated that TQSB exhibited a high selectivity toward Al, as observed from its excellent binding constant ( = 3.8 × 10 M) and detection limit (7.

An Indole-based Chromofluorogenic Probe for Detection of Trivalent Al, Ga, In and Fe Ions.

An easily synthesizable indole-derived chromofluorogenic probe InNS has been demonstrated for recognition of trivalent metal ions (i. e., Al, Ga, In and Fe).

Indium nanocubes based recyclable fluorescent chemosensor for sustainable environmental monitoring: pH-induced fluorescence transition and selective detection of Pd(II) ions.

Rapid modern industrialization and urbanization have escalated heavy metal pollution, with palladium (Pd) raising significant concerns due to its extensive usage in catalysis, hydrogen storage, and electronics, thereby imposing substantial risks on the environment and human health. In this study, we report a highly fluorescent indium nanocubes based chemosensor (InNCs) functionalized with perylene tetracarboxylic acid (PTCA) and 4-(pyridyl)ethenyl benzene (PEB). The InNCs exhibited emission maximum at 415 nm (λ ∼ 350 nm) with robust chemical and photo-stability, and acted as a fluorogenic probe for selective recognition of Pd in aqueous medium.

A quinoline derived Schiff base as highly selective 'turn-on' probe for fluorogenic recognition of Al ion.

A quinoline-derived Schiff base QnSb has been synthesized for fluorescent and colorimetric recognition of Al ions in a semi-aqueous medium. The compound QnSb has been characterized by elemental analysis, FT-IR, H/C NMR, UV-Vis and fluorescence spectral techniques. The crystal structure of the QnSb was confirmed by single crystal X-ray diffraction (SC-XRD) analysis.

Turn-on detection of Al and Zn ions by a NSN donor probe: reversibility, logic gates and DFT calculations.

An efficient dual functional naphthalene-derived Schiff base NpSb probe has been synthesised and evaluated for its fluorescence and chromogenic response towards metal ions. The NpSb probe was capable of selectively recognising Al and Zn ions when they were excited at the same wavelength in an aqueous organic solvent system. Almost non-fluorescent NpSb displayed a 'turn-on' fluorescence response when treated with Zn ( = 416 nm) and Al ( = 469 nm) ions due to the chelation-enhanced fluorescence (CHEF) effect.

Fluorescent and chromogenic organic probes to detect group 10 metal ions: design strategies and sensing applications.

Group 10 metals including Ni, Pd and Pt have been extensively applied in various essential aspects of human social life, material science, industrial manufactures, medicines and biology. The ionic forms of these metals are involved in several biologically important processes due to their strong binding capability towards different biomolecules. However, the mishandling or overuse of such metals has been linked to serious contamination of our ecological system, more specifically in soil and water bodies with acute consequences.

Development of low-cost copper nanoclusters for highly selective "turn-on" sensing of Hg ions.

The development of low-cost earth abundant metal based fluorescent sensors for a rapid and selective nanomolar level detection of Hg is essential due to the increasing world-wide concern of its detrimental effect on humans as well as the environment. Herein, we present a perylene tetracarboxylic acid functionalized copper nanoclusters (CuNCs) based "turn-on" fluorescence probe for highly selective detection of toxic Hg ions. The fabricated CuNCs exhibited high photostability with emission maximum centered at 532 nm (λ = 480 nm).

An Electroanalytical Flexible Biosensor Based on Reduced Graphene Oxide-DNA Hybrids for the Early Detection of Human Papillomavirus-16.

Human Papilloma Virus 16 (HPV 16) is the well-known causative species responsible for triggering cervical cancer. When left undiagnosed and untreated, this disease leads to life-threatening events among the female populace, especially in developing nations where healthcare resources are already being stretched to their limits. Considering various drawbacks of conventional techniques for diagnosing this highly malignant cancer, it becomes imperative to develop miniaturized biosensing platforms which can aid in early detection of cervical cancer for enhanced patient outcomes.

Regulatory T cells (Tregs) and their therapeutic potential against autoimmune disorders - Advances and challenges.

Autoimmune diseases are caused when immune cells act against self-protein. This biological self-non-self-discrimination phenomenon is controlled by a distinct group of lymphocytes known as regulatory T cells (Tregs), which are key inflammatory response regulators and play a pivotal role in immune tolerance and homeostasis. Treg-mediated robust immunosuppression provides self-tolerance and protection against autoimmune diseases.

Immunization with S19Δ Conferred Protection in Water Buffaloes against Virulent Challenge with Strain S544.

Vaccination of cattle and buffaloes with strain 19 has been the mainstay for control of bovine brucellosis. However, vaccination with S19 suffers major drawbacks in terms of its safety and interference with serodiagnosis of clinical infection. S19∆, a perosamine synthetase B gene deletion mutant, overcomes the drawbacks of the S19 vaccine strain.

Bioinspired Heterobimetallic Photocatalyst () for Visible-Light-Driven C-H Oxidation of Organic Substrates via Dioxygen Activation.

We report a bioinspired heterobimetallic photocatalyst and its relevant applications toward visible-light-driven C-H bond oxidation of a series of hydrocarbons using O as the O-atom source. The Ru center absorbs visible light near 460 nm and triggers a cascade of electrons to Fe to afford a catalytically active high-valent Fe═O species. The formed Fe═O has been employed for several high-impact oxidation reactions in the presence of triethanolamine (TEOA) as the sacrificial electron donor.

Differential responses of chicken monocyte-derived dendritic cells infected with Salmonella Gallinarum and Salmonella Typhimurium.

Salmonella enterica serovar Gallinarum is a host-restricted bacterial pathogen that causes a serious systemic disease exclusively in birds of all ages. Salmonella enterica serovar Typhimurium is a host-generalist serovar. Dendritic cells (DCs) are key antigen-presenting cells that play an important part in Salmonella host-restriction.

Brucella abortus S19 rfbD mutant is highly attenuated, DIVA enable and confers protection against virulent challenge in mice.

Brucella abortus S19 is an important tool for controlling bovine brucellosis across the globe. However, vaccination with S19 suffers critical shortcomings such as, presence of residual virulence, induction of abortion and sero-diagnostic interference. In this study, rfbD gene deleted mutant S19 was developed.

Combination of TLR2 and TLR3 agonists derepress infectious bursal disease virus vaccine-induced immunosuppression in the chicken.

Live intermediate plus infectious bursal disease virus (IBDV) vaccines (hot vaccines) are used for protection against the virulent IBDV strains in young chickens. We evaluated the potential of Toll-like receptor (TLR) agonists to alleviate hot vaccine-induced immunosuppression. The combination of Pam3CSK4 and poly I:C synergistically upregulated IFN-β, IFN-γ, IL-12, IL-4, and IL-13 transcripts and cross-inhibited IL-1β, IL-10, and iNOS transcripts in the chicken peripheral blood mononuclear cells (PBMCs) as analyzed by quantitative real-time PCR.

Development and evaluation of a gold nanoparticle-based immunochromatographic strip test for the detection of canine parvovirus.

Canine parvovirus (CPV) is the leading viral cause of enteritis in dogs and occurs mainly in 6- to 8-week-old pups. Rapid diagnosis of CPV under field conditions is now possible due to commercially available immunochromatographic (IC) assays. However, these commercial kits are somewhat expensive because they utilize a minimum of two monoclonal antibodies (mAbs) targeting different epitopes as capture and detector antibodies.

In vitro And In vivo Immunomodulating Properties of Mesenchymal Stem Cells.

Background: Mesenchymal Stem Cells (MSCs) are self-renewing, multipotent progenitor cells with multilineage potential to differentiate into all cell types of mesodermal origin, such as adipocytes, osteocytes and chondrocytes. Mesenchymal Stem Cells (MSCs) are adult stem cells which can be isolated from human and animal sources.

Objective: Besides the differentiation potential of MSCs, these also regulate the immune response in numerous ailments.

Role of different receptors and actin filaments on Salmonella Typhimurium invasion in chicken macrophages.

Bacterial attachment to host cell is the first event for pathogen entry. The attachment is mediated through membrane expressed adhesins present on the organism and receptors on the cell surface of host. The objective of this study was to investigate the significance of Fc receptors (FcRs), actin filament polymerization, mannose receptors (MRs), carbohydrate moieties like N-linked glycans and sialic acid on chicken macrophages for invasion of S.

Effect of dietary mannanoligosaccharide supplementation on nutrient digestibility, hindgut fermentation, immune response and antioxidant indices in dogs.

Background: Use of prebiotics in companion animal nutrition is often considered advantageous over probiotics because of the ease of handling, ability to withstand processing and storage etc. While most of the studies on prebiotic use in dogs have been done with processed food as basal diet, the response in relation to homemade diet feeding is not very well explored.

Methods: The study was conducted to evaluate the effects of dietary mannanoligosaccharide (MOS) supplementation on nutrient digestibility, hindgut fermentation, immune response and antioxidant indices in dogs.

Protein-L-Isoaspartyl Methyltransferase (PIMT) Is Required for Survival of Typhimurium at 42°C and Contributes to the Virulence in Poultry.

Poultry birds are asymptomatic reservoir of Typhimurium (. Typhimurium) but act as source of human infection for this bacterium. Inside the poultry, .

Salmonella Typhimurium methionine sulfoxide reductase A (MsrA) prefers TrxA in repairing methionine sulfoxide.

Intraphagocytic survival of Salmonella Typhimurium (ST) depends (at least in part) upon its ability to repair oxidant-damaged macromolecules. Met residues either free or in protein bound form are highly susceptible to phagocyte-generated oxidants. Oxidation of Mets leads to Met-SO formation, consequently loss of protein functions that results in cell death.

Contribution of protein isoaspartate methyl transferase (PIMT) in the survival of Salmonella Typhimurium under oxidative stress and virulence.

The enteric pathogen Salmonella Typhimurium (ST) survives inside the oxidative environment of phagocytic cells. Phagocyte generated oxidants primarily target proteins and modify amino acids in them. These modifications render the targeted proteins functionally inactive.

Methionine sulfoxide reductase A (MsrA) contributes to Salmonella Typhimurium survival against oxidative attack of neutrophils.

Salmonella Typhimurium (ST) must evade neutrophil assault for infection establishment in the host. Myeloperoxidase generated HOCl is the key antimicrobial agent produced by the neutrophils; and methionine (Met) residues are the primary targets of this oxidant. Oxidation of Mets leads to methionine sulfoxide (Met-SO) formation and consequently compromises the protein function(s).