Inhibitory Effect of Aconitine on Colorectal Cancer Malignancy via Inducing Apoptosis and Suppression of Cell Motion.

Background/aims: The incidence of colorectal cancer (CRC) has been increasing in recent years worldwide. Aconitine is a diester diterpenoid alkaloid that exhibits an antitumor role in several cancers. Nevertheless, it remains unclear whether aconitine also has antitumor activity in CRC.

Association between physical activity and visceral adiposity index (VAI) in U.S. population with overweight or obesity: a cross-sectional study.

Background: Previous studies have revealed the effects of different physical activity (PA) types on visceral adipose tissue (VAT) accumulation in individuals with overweight/obesity. However, the independent association (especially the dose-response relationship) between PA and VAT in individuals with and without overweight/obesity remains less explored. Visceral adiposity index (VAI), calculated from waist circumference, body mass index (BMI), triglyceride and high-density lipoprotein cholesterol, is a novel indicator of VAT.

Complete coding genome sequence of a genotype strain.

This study reports the complete coding genome sequence of a novel genotype strain, SG2, isolated from the fecal sample of an infected indigenous pig in western Jiangxi, China.

Starch multiscale structure and physicochemical property alterations in high-quality indica rice quality and cooked rice texture under different nitrogen panicle fertilizer applications.

The starch multiscale structure, physiochemical properties, grain quality and cooked rice texture of high-quality early and late indica were analyzed under nitrogen panicle fertilizer (low panicle fertilizer, LPF; middle panicle fertilizer, MPF; high panicle fertilizer, HPF) treatments and their internal relations were investigated. Compared to the MPF treatment, the starch granules in HPF and LPF had more surface-proteins and irregular voids for high-quality early and late indica rice cultivars, respectively. Nitrogen panicle fertilization application increased amylopectin medium and long chains as well as protein content, resulting in higher relative crystallinity and gelatinization temperatures.

Grain yield and quality performances of different late-season rice cultivars in response to experimental warming in subtropical China.

Introduction: Climate warming has pronounced effects on rice production in China. However, late-seasons rice cultivars are diverse in double rice cropping systems, and the actual responses in grain yield and quality of different late-season rice cultivars to climate warming are still unclear.

Methods: A two-year field warming experiment was conducted by using free-air temperature increase facilities with three widely-planted late-season rice cultivars, including Taiyou398 (TY, short growth duration hybrid rice), Jiuxiangnian (JXN, long growth duration inbred rice), and Yongyou1538 (YY, long growth duration hybrid rice) in a double rice cropping system in subtropical China.

All-printed multiplexed electrocatalytic biosensors with rationally designed nanoparticle inks.

Inkjet printing, capable of rapid and template-free fabrication with high resolution and low material waste, is a promising method to construct electrochemical biosensor devices. However, the construction of fully inkjet-printed electrochemical biosensor remains a challenge owing to the lack of appropriate inks, especially the sensing inks of bioactive materials. Herein, we demonstrate a fully inkjet-printed, integrated and multiplexed electrochemical biosensor by combining rationally designed nanoparticle Inks.

PEDOT:PSS hydrogels with high conductivity and biocompatibility for cell sensing.

Conducting polymer hydrogels, especially poly(3,4-ethylenedioxythiophene):poly(styrene sulfonate) (PEDOT:PSS) hydrogels, show great promise in soft bioelectronics due to their high conductivity and the ability of electrical coupling with tissues for sensing and stimulation. However, it is challenging to solve the problem of poor biocompatibility of PEDOT:PSS hydrogels due to the existing harsh preparation methods with the use of toxic and harmful reagents. Herein, we report the synthesis of PEDOT:PSS hydrogels with positively charged conductive polymers as a cross-linker and the application of PEDOT:PSS hydrogels as electrochemical sensors for living cells.

Genome-resolved metagenomics reveals depth-related patterns of microbial community structure and functions in a highly stratified, AMD overlaying mine tailings.

Acid mine drainage (AMD) is a worldwide environmental problem, yet bioremediation is hampered by a limited knowledge of the reductive microbial processes in the AMD ecosystem. Here, we generate extensive metagenome and geochemical datasets to investigate how microbial populations and metabolic capacities driving major element cycles are structured in a highly stratified, AMD overlaying tailings environment. The results demonstrated an explicit depth-dependent differentiation of microbial community composition and function profiles between the surface and deeper tailings layers, paralleling the dramatic shifts in major physical and geochemical properties.

Analysis of the Influence of Network Continuous Care on the Quality of Life of Patients with Coronary Artery Disease (CAD) after PIC.

Studies have shown that most patients after PCI cannot adhere to the cardiac rehabilitation program. The survey found that due to the lack of secondary prevention of cardiac rehabilitation, the phenomenon of drug reduction and withdrawal after PCI is very common after discharge, leading to recurrence of the patient's disease or worsen and repeated hospitalizations, so continuity of care is very important. In this paper, in addition to proposing a network care continuum with artificial intelligence handler in order to improve the healthcare system and provide new ideas for improving the postoperative recovery of CHD patients, we analyze the impact of PCI on the coronary heart disease patients' PCI postoperative quality of life.

Relation of cooked rice texture to starch structure and physicochemical properties under different nitrogen managements.

Nitrogen management, especially panicle nitrogen fertilization (PNF), can affect cooked rice textures by altering starch quality, but the details are unclear. In this study, the starch multi-level structures, physicochemical properties and cooked rice textures of indica under different nitrogen managements were analysed and their internal relations were investigated. With an increase in PNF, large granules, amylopectin short chains, amylose content, thermal temperatures, setback, and hardness first exhibited decreasing trends and then increasing trends, which were relatively lower under moderate PNF (N3), whereas α-1,6 linkage, relative crystallinity, protein content, enthalpy of gelatinization (△H), peak viscosity, breakdown and stickiness exhibited the opposite trends.

Template-free, controllable and scalable synthesis of poly(5-aminoindole) nanoparticles for printable electrochemical immunosensor with ultra-high sensitivity.

Conductive polymer polyindole derivatives have good conductivities and abundant functional groups, which would offer great potential for versatile applications including biosensors, bioelectronics and energy devices. However, the polyindole derivatives are mainly synthesized by the electropolymerization method on conductive electrode surfaces, which limits large-scale synthesis and practical applications. Herein, we explore a strategy of template-free, controllable and scalable synthesis of poly-5-aminoindole (PIn-5-NH) nanoparticles (NPs) and demonstrate the application of PIn-5-NH NPs in printable multiplexed electrochemical biosensors with ultra-high sensitivity.

Characterization of porcine sapelovirus prevalent in western Jiangxi, China.

Background: Porcine sapelovirus (PSV) infection can lead severe polioencephalomyelitis with high morbidity and mortality, which result in significant economic losses. Infection with the PSV is believed to be common yet limited information is available on the prevalence and molecular characterization of PSV in China. Therefore, the objective of this study was to characterize the prevalence and genome of PSV strains identified in the western Jiangxi province of China.

Poly(indole-5-carboxylic acid)/reduced graphene oxide/gold nanoparticles/phage-based electrochemical biosensor for highly specific detection of Yersinia pseudotuberculosis.

Yersinia pseudotuberculosis is an enteric bacterium causing yersiniosis in humans. The existing Yersinia pseudotuberculosis detection methods are time-consuming, requiring a sample pretreatment step, and are unable to discriminate live/dead cells. The current work reports a phage-based electrochemical biosensor for rapid and specific detection of Yersinia pseudotuberculosis.

The role of intestinal microbiota of the marine fish (Acanthopagrus latus) in mercury biotransformation.

Both inorganic (IHg) and organic (MeHg) forms of Hg can be converted into each other by methylation or demethylation, leading to changes of Hg speciation and distribution in fish. However, Hg biotransformation in fish is not thoroughly understood and the key factors in this process are unclear. The present study investigated the in vivo Hg transformation in a marine fish (Acanthopagrus latus) and explored the roles of intestinal microbiota in Hg biotransformation.

Schisandrin B Protects against Acute Ethanol-Induced Cardiac Injury by Downregulating Autophagy via the NOX4/ROS Pathway.

Introduction: Although oxidative stress has been demonstrated to mediate acute ethanol-induced changes in autophagy in the heart, the precise mechanism behind redox regulation in acute ethanol heart disease remains largely unknown.

Methods: Wild-type C57BL/6 mice were intraperitoneally injected with ethanol (3 g/kg/day) for 3 consecutive days. The effects of ethanol on cultured primary cardiomyocytes and H9c2 myoblasts were also studied in vitro.

Water irrigation management affects starch structure and physicochemical properties of indica rice with different grain quality.

The effects of water irrigation management including conventional irrigation (CK), constant flooding irrigation (CFI) and alternate wetting and drying (AWD) on starch structure and physicochemical properties of two indica rice cultivars with good- and poor-quality were evaluated in the field condition with two years. The results showed that AWD could significantly increase peak viscosity, breakdown and gelatinization temperature, decreased setback and gelatinization enthalpy in two indica rice cultivars. However, starch granule size and amylopectin chain length distribution were differed the trends in the rice cultivars and treatments.

Revegetation approach and plant identity unequally affect structure, ecological network and function of soil microbial community in a highly acidified mine tailings pond.

Owing to its sustainability and low cost, direct revegetation (DR) has been considered a promising alternative to capped revegetation (CR) for dealing with the serious environmental problem derived from various types of mine wastelands that are widespread in the world. However, a direct comparison of the performance of these two revegetation approaches for reclamation of extremely acidic mine wastelands and the underlying mechanisms is still lacking. To bridge this critical knowledge gap, we established 5000 m of vegetation on a highly acidified (pH < 3) Pb/Zn mine tailings pond employing both CR and DR schemes (2500 m for each scheme).

A highly sensitive label-free electrochemical immunosensor based on an aligned GaN nanowires array/polydopamine heterointerface modified with Au nanoparticles.

Aligned GaN nanowire arrays show great potential not only in optoelectronic devices, but also in sensitive biosensor applications, owing to their excellent chemical stability and biocompatibility, as well as high electron mobility and surface-to-volume ratio. However, to construct electrochemical immunosensors, proper surface modification of GaN nanowires, which can enable efficient charge transfer and provide large densities of immobilization sites for antibodies to anchor, is still challenging. Herein we demonstrate a highly sensitive label-free electrochemical immunosensing platform based on the integration of polydopamine (PDA) on a GaN nanowire surface.