Fluorescence-Based Flow Sorting in Parallel with Transposon Insertion Site Sequencing Identifies Multidrug Efflux Systems in Acinetobacter baumannii.

Unlabelled: Multidrug efflux pumps provide clinically significant levels of drug resistance in a number of Gram-negative hospital-acquired pathogens. These pathogens frequently carry dozens of genes encoding putative multidrug efflux pumps. However, it can be difficult to determine how many of these pumps actually mediate antimicrobial efflux, and it can be even more challenging to identify the regulatory proteins that control expression of these pumps.

Effects of Notch2 and Notch3 on Cell Proliferation and Apoptosis of Trophoblast Cell Lines.

Aims: To investigate the effect of Notch2 and Notch3 on cell proliferation and apoptosis of two trophoblast cell lines, BeWo and JAR.

Methods: Notch2 and Notch3 expression in BeWo and JAR cells was upregulated or downregulated using lentivirus-mediated overexpression or RNA interference. The effect of Notch2 and Notch3 on cell proliferation was assessed by the CCK-8 assay.

Expression of notch family proteins in placentas from patients with early-onset severe preeclampsia.

Objectives: This study is aimed to identify the expression of Notch family proteins in placentas from patients with early-onset severe preeclampsia.

Study Design: The expression of Notch family proteins in placentas was investigated by immunohistochemistry, Western blotting, and real-time reverse transcription-polymerase chain reaction (RT-PCR).

Results: The profile of distribution of all Notch family proteins in placentas from patients with early-onset severe preeclampsia is similar to that in normal placentas.

[Analysis of clinical features of pregnant women with hypertrophic cardiomyopathy].

Objective: To discuss the clinical features of pregnant women with hypertrophic cardiomyopathy (HCM).

Methods: There were 28 patients with HCM who delivered in Renji hospital of Shanghai Jiaotong University from January 2000 to August 2012. Clinical data were analyzed, including diagnosis, cardiac functional grading, gestational weeks of delivery, delivery mode, birth weight, Apgar scores, etc.

Risk factors and risk index of cardiac events in pregnant women with heart disease.

Background: Pregnant women with heart disease are at high risk. Studies of risk factors of these patients are of great significance to improve maternal and fetal outcomes. In this paper, we try to discuss the main risk factors of cardiac events in pregnant women with heart disease and to establish a risk assessment system.

Effect of aqueous extract of Arctium lappa L. (burdock) roots on the sexual behavior of male rats.

Background: Arctium lappa L. root has traditionally been recommended as an aphrodisiac agent. It is used to treat impotence and sterility in China, and Native Americans included the root in herbal preparations for women in labor.