Publications by authors named "Tao-di Liu"
[Comparative study of two high-efficiency methods for purifying spermatogonial stem cells].
Zhongguo Ying Yong Sheng Li Xue Za Zhi
September 2021
Article Synopsis
- The study compares two methods for purifying mouse spermatogonial stem cells (mSSCs): the differential attachment method and the immunomagnetic bead method.
- The immunomagnetic bead method yields a higher number of mSSCs with a recovery rate of about 3%, while the differential attachment method produces purer stem cells with a lower recovery rate of 1.5%.
- Both methods have their own pros and cons, with the differential attachment method being more economical and practical as it doesn't need specialized equipment.
Objective: To study the expression of the Ces5a gene in the development of the rat testis.
Methods: Using RT-PCR, Western blot, immunohistochemistry and HE staining, we determined the mRNA transcription level, protein expression and localization of the Ces5a gene in the testes of three litters of rats at different postnatal (PN) days.
Results: The expression of Ces5a mRNA was found in the testis tissue of the rats at 2－65 PN days, low at 2－12 days, decreased to the lowest level at 14－16 days (P < 0.
[Expression of the MYL2 gene in the development of rat testis tissue].
Zhonghua Nan Ke Xue
March 2018
Objective: To study the expression of the gene of myosin regulatory light chain-2 (MYL2) in the development of rat testis tissue.
Methods: Using real-time PCR and immunohistochemistry, we determined the mRNA transcription level and protein expression of MYL2 in the rat testis.
Results: The mRNA expression of the MYL2 gene changed in an age-dependent manner, reaching the highest value on postnatal day (PND) 2, then dropped rapidly till PND 8, increased slowly on PNDs 10 and 12, decreased on PND 14, rose slightly from PND 15 and rapidly on PNDs 20 and 25, and declined slowly from PND 65.
[Effect of dopamine receptor agonist apomorphine on scopolamine induced memory deficits in mice].
Zhongguo Ying Yong Sheng Li Xue Za Zhi
May 2014
Objective: To research the mechanism of dopamine (DA) controlled memory in mice.
Methods: Mice received i.p.