Confirmation of ovarian follicles in an enantiornithine (Aves) from the Jehol biota using soft tissue analyses.

The remains of ovarian follicles reported in nine specimens of basal birds represents one of the most remarkable examples of soft-tissue preservation in the Early Cretaceous Jehol Biota. This discovery was immediately contested and the structures alternatively interpreted as ingested seeds. Fragments of the purported follicles preserved in an enantiornithine (STM10-12) were extracted and subjected to multiple high-resolution analyses.

[-regulatory mechanisms and biological effects of translation elongation].

Proteins are biological macromolecules essential for cells to maintain their metabolic activities. Proteins are synthesized during translation elongation, a synergistic process in which ribosomes decode the genetic information transmitted in mRNA, using tRNA. Numerous human diseases, such as neurodegenerative diseases and cancers, are known to be related to abnormal translation elongation.

Characterization and expression analysis of seven selenoprotein genes in yellow catfish Pelteobagrus fulvidraco to dietary selenium levels.

Background: Selenium (Se) appears in the selenoproteins in the form of selenocysteine (Sec) and is important for the growth and development of vertebrates. The present study characterized seven selenoproteins, consisting of the GPX1, GPX3, GPX4, SELENOW, SELENOP, TXNRD2 and TXNRD3 cDNAs in various tissues of yellow catfish, explored their regulation to dietary Se addition.

Methods: 3' and 5' RACE PCR were used to clone full-length cDNA sequences of seven selenoprotein genes (GPX1, GPX3, GPX4, SELENOW, SELENOP, TXNRD2 and TXNRD3).

Effects of dietary carbohydrate sources on lipid metabolism and SUMOylation modification in the liver tissues of yellow catfish.

Dysregulation in hepatic lipid synthesis by excess dietary carbohydrate intake is often relevant with the occurrence of fatty liver; therefore, the thorough understanding of the regulation of lipid deposition and metabolism seems crucial to search for potential regulatory targets. In the present study, we examined TAG accumulation, lipid metabolism-related gene expression, the enzyme activities of lipogenesis-related enzymes, the protein levels of transcription factors or genes involving lipogenesis in the livers of yellow catfish fed five dietary carbohydrate sources, such as glucose, maize starch, sucrose, potato starch and dextrin, respectively. Generally speaking, compared with other carbohydrate sources, dietary glucose promoted TAG accumulation, up-regulated lipogenic enzyme activities and gene expressions, and down-regulated mRNA expression of genes involved in lipolysis and small ubiquitin-related modifier (SUMO) modification pathways.

Factors driving the change of household CO emissions through 2040 in China: based on decomposition and scenario analyses.

With China's economic transformation into a consumption-driven model, household CO emissions (HCEs) become an increasingly essential part of China's CO emissions. However, how will the HCEs change in urban and rural areas and what are the driving forces of HCEs through 2040 are not clear. In this paper, we project nation-level HCEs up to 2040 by the scenario analysis, and analyze the drivers of HCEs during 1997-2040 by the decomposition analysis.

tDCS Accelerates the Rehabilitation of MCAO-Induced Motor Function Deficits via Neurogenesis Modulated by the Notch1 Signaling Pathway.

Ischemic stroke carries a high mortality rate and is a leading cause of severe neurological disability. However, the efficacy of current therapeutic options remains limited. We aimed to investigate the treatment efficacy of transcranial direct current stimulation (tDCS) in motor function rehabilitation after ischemic stroke and explore the underlying mechanisms.

POU4F1 promotes the resistance of melanoma to BRAF inhibitors through MEK/ERK pathway activation and MITF up-regulation.

BRAF inhibitors (BRAFi) have shown remarkable clinical efficacy in the treatment of melanoma with BRAF mutation. Nevertheless, most patients end up with the development of BRAFi resistance, which strongly limits the clinical application of these agents. POU4F1 is a stem cell-associated transcriptional factor that is highly expressed in melanoma cells and contributes to BRAF-activated malignant transformation.

Understanding regional inequality in per capita CO emissions in China during 1997-2016: sources and driving factors.

As the largest carbon dioxide (CO) emitter, China exists obvious regional inequality in per capita CO emissions. However, such inequality and its dynamic change for recent years have not been systematically studied. In this paper, we evaluate China's regional inequality in per capita CO emissions during 1997-2016 using the Theil index and decompose it into within-region and between-region components based on eight regions.

Extraction of tetracycline in food samples using biochar microspheres prepared by a Pickering emulsion method.

We report for the first time the use of biochar as a stabilizer for oil-in-water (o/w) Pickering emulsion. The emulsion is subsequently used to prepare tetracycline-imprinted biochar composite microspheres (MIPMs) with tailored sizes and good uniformity. The adsorption properties of tetracycline to the MIPMs were investigated using different adsorption experiments including adsorption kinetic experiment, equilibrium binding experiment, selectivity evaluation and competitive adsorption tests.

Efficacy evaluation of different conservative treatments for blunt spleen rupture.

Background: This study aimed to provide a reference for the clinical treatment of patients with spleen ruptures by analyzing and discussing the clinical effects of the conservative treatment.

Methods: The clinical data of 93 patients with blunt spleen rupture treated in the First Affiliated Hospital of University of Science and Technology of China from April 2015 to April 2018 were retrospectively analyzed. Among them, 84 cases were treated conservatively and 9 cases were treated surgically.

A Mild Method for Preparation of Highly Selective Magnetic Biochar Microspheres.

We report the use of biochar and FeO nanoparticles as co-stabilizers for oil-in-water (o/w) Pickering emulsion. The emulsion is subsequently used to prepare magnetic tetracycline-imprinted biochar composite microspheres (MMIPMs) with good uniformity and high selectivity. The MMIPMs were characterized by scanning electron microscopy (SEM), Brunner-Emmet-Teller (BET) measurements, Fourier transform infrared spectrometry (FT-IR), X-ray diffraction (XRD), vibrating sample magnetometer (VSM) and thermogravimetry analysis (TGA).

The impact of technology-environmental innovation on CO emissions in China's transportation sector.

Along with the development of urbanization and informationization, an increasing attention has been attracted to CO emissions of China's transportation sector and its influencing factors. Such researches mainly utilize single indicator or two indicators to represent technology process. This research aims to verify the influence of technology-environmental innovation indicator system on CO emissions of China's transportation sector by decoupling elasticity and econometric model.

Effect of tetracaine on dynamic reorganization of lipid membranes.

To understand the intrinsic influence of a drug on lipid membranes is of critical importance in pharmacological science. Herein, we report fluorescence microscopy analysis of the interaction between the local anesthetic tetracaine (TTC) and planar supported lipid bilayers (SLBs), as model membranes. Our results show that TTC increases lipid chain mobility, destabilizes the SLBs and remarkably induces membrane disruption and solubilization.

COVID-19 with rheumatic diseases: a report of 5 cases.

The coronavirus disease 2019 (COVID-19), the result of an infection with the new virus, SARS-CoV-2, is rapidly spreading worldwide. It is largely unknown whether the occurrence of COVID-19 in patients with rheumatic immune diseases has some specific manifestations, or makes them more prone to rapidly progress into severe COVID-19. In this case report, we describe the clinical features of 5 rheumatic immune disease patients with the concomitant presence of COVID-19.

Molecular underpinnings of methyl jasmonate-induced resistance in Norway spruce.

In response to various stimuli, plants acquire resistance against pests and/or pathogens. Such acquired or induced resistance allows plants to rapidly adapt to their environment. Spraying the bark of mature Norway spruce (Picea abies) trees with the phytohormone methyl jasmonate (MeJA) enhances resistance to tree-killing bark beetles and their associated phytopathogenic fungi.

Investigation of a New Catheter on Relieving Pain During Male Cystoscopy - A Randomized Clinical Trial.

Purpose: To investigate the pain intensity and tolerability of a new catheter applied to urethral surface anesthesia during rigid cystoscopy in male patients, and explore the prospects of its application and the anesthetic method in hospitals at primary levels.

Materials And Methods: 252 adult male patients were randomly divided into the experimental group and the control group.1% lidocaine solution was irrigated into the posterior urethra of the experimental group using the new catheter before cystoscopy, while the control group was administered with lidocaine gel.

Preparation of polydopamine-coated, graphene oxide/Fe O - imprinted nanoparticles for selective removal of sulfonylurea herbicides in cereals.

Background: Sulfonylureas are potentially toxic broad-spectrum herbicides. They pose a persistent threat to food safety and the environment. It is therefore important to develop a rapid and efficient pretreatment and detection method to prevent their harmful effects on human health.

Waterborne Cu exposure increased lipid deposition and lipogenesis by affecting Wnt/β-catenin pathway and the β-catenin acetylation levels of grass carp Ctenopharyngodon idella.

Lipid metabolism could be used as a biomarker for environmental monitoring of metal pollution, including Cu. Given the potential role of the Wnt/β-catenin signaling pathway and acetylation in lipid metabolism, the aim of this study was to investigate the mechanism of Wnt signaling and acetylation mediating Cu-induced lipogenesis. Grass carp Ctenopharyngodon idella, widely distributed freshwater teleost, were used as the model.

Impact of Interferon-alpha1b (IFN-α1b) on Antitumor Immune Response: An Interpretation of the Promising Therapeutic Effect of IFN-alpha1b on Melanoma.

BACKGROUND Melanoma is among the most aggressive forms of cancer. Our latest retrospective analysis showed that recombinant human interferon-alpha1b (IFN-alpha1b) led to significantly prolonged survival with mild toxicity in patients with stage IV melanoma. Based on this clinical finding, the current study sought to investigate the influence of IFN-alpha1b on the antitumor immunity of melanoma, with interferon-alpha2b (IFN-alpha2b) used as a control.

FXR-mediated inhibition of autophagy contributes to FA-induced TG accumulation and accordingly reduces FA-induced lipotoxicity.

Background: Excessive dietary fat intake induces lipid deposition and contributes to the progress of nonalcoholic fatty liver disease (NAFLD). However, the underlying mechanisms are still unclear.

Methods: Yellow catfish were given two experimental diets with dietary lipid levels of 11.

The Involvement of Type 2 Innate Lymphoid Cells in Airway Inflammation of Asthma.

The airway inflammatory response is closely associated with asthma. The purpose of this article was to study the roles of innate lymphoid cells (ILCs) in the process of airway inflammatory response in asthma. We established the asthmatic mice model with intraperitoneal injected ovalbumin medium, then with the flow cytometry analysis, we detected the ILCs and their surface proteins in the mice blood samples, besides, we analyzed the amounts of inflammatory cytokines and secreted proteins in the mice bronchoalveolar lavage fluid and blood serum.

Preparation of hydrophilic molecularly imprinted solid-phase microextraction fiber for the selective removal and extraction of trace tetracyclines residues in animal derived foods.

The present work reported a novel hydrophilic and selective solid-phase microextraction fiber by improved multiple co-polymerization method immobilization of tetracycline molecularly imprinted polymer on a stainless steel wire and directly coupled with high-performance liquid chromatography for sensitive determination of trace tetracyclines residues in animal derived foods. The developed molecularly imprinted polymer coated solid-phase microextraction fibers were characterized through scanning electron microscopy, Fourier transfer infrared spectroscopy, thermogravimetric analysis, and adsorption experiments, the fiber with cross-linked and porous structure was observed and high thermal and chemical stability. The maximum adsorption capacity of this fiber with good selectivity reached 2.

From Discriminant to Complete: Reinforcement Searching-Agent Learning for Weakly Supervised Object Detection.

Weakly supervised object detection (WSOD) is an interesting yet challenging task in the computer vision community. The core is to discover the image regions that contain the complete object instances under the image-level supervision. Existing works usually solve this problem via a proposal selection strategy, which selects the most discriminative box regions from the weakly labeled training images.

ATP-Citrate Lyase Epigenetically Potentiates Oxidative Phosphorylation to Promote Melanoma Growth and Adaptive Resistance to MAPK Inhibition.

Purpose: Enhanced lipogenesis and mitochondrial function are two critical metabolic characteristics in melanoma, but their crosstalk involved in tumor biology and targeted therapy remains unknown. ATP-citrate lyase (ACLY) is a crucial lipogenic enzyme that is greatly implicated in tumor development, but its role in mitochondrial function and melanoma pathogenesis has not been elucidated.

Experimental Design: and functional experiments were performed to determine the effect of ACLY on melanoma growth.