Retrospective analysis of clinical and pathologic risk factors in liver resection for hepatic colorectal metastases.

Objective: To analyze the clinical and pathologic risk factors of surgical treatment for liver metastases from colorectal cancer.

Materials And Methods: The data on 98 patients who underwent liver resection for hepatic colorectal metastases were collected and analyzed retrospectively.

Results: Overall 1-, 3-, and 5-year survival rates after hepatectomy for metastases were 94.

Therapeutic potentials of gene silencing by RNA interference: principles, challenges, and new strategies.

During recent decades there have been remarkable advances in biology, in which one of the most important discoveries is RNA interference (RNAi). RNAi is a specific post-transcriptional regulatory pathway that can result in silencing gene functions. Efforts have been done to translate this new discovery into clinical applications for disease treatment.

Isolation and characterization of pyrene and benzo[a]pyrene-degrading Klebsiella pneumonia PL1 and its potential use in bioremediation.

Polycyclic aromatic hydrocarbons (PAHs), which are hard to degrade, are the main pollutants in the environment. Degradation of PAHs in the environment is becoming more necessary and urgent. In the current study, strain PL1 with degradation capability of pyrene (PYR) and benzo[a]pyrene (BaP) was isolated from soil and identified as Klebsiella pneumoniae by morphological and physiological characteristics as well as 16S rDNA sequence.

[Relationship between colorectal adenocarcinoma with invasive micropapillary carcinoma component and lymph node metastasis].

Objective: To investigate the relationship between colorectal adenocarcinoma with invasive micropapillary carcinoma (IMPC) component, lymphovascular invasion, and lymph node metastasis.

Methods: One hundred and thirty one cases of colorectal adenocarcinoma with invasive micropapillary carcinoma component were evaluated by H&E and immunohistochemical staining. The main pathological features, percentage of IMPC component, lymphovascular invasion, and lymph node metastasis were assessed and compared to 296 cases of conventional colorectal adenocarcinoma.

Therapeutic RNA interference targeting CKIP-1 with a cross-species sequence to stimulate bone formation.

Objectives: Casein kinase 2 interacting protein 1 (CKIP-1) is a newly discovered intracellular negative regulator of bone formation without affecting bone resorption. In this study, we aimed to identify a cross-species siRNA sequence targeting CKIP-1 to facilitate developing a novel RNAi-based bone anabolic drug for reversing established osteoporosis.

Methods: Eight specifically designed cross-species CKIP-1 siRNA sequences were screened in human, rhesus, rat and mouse osteoblast-like cells in vitro to identify the optimal sequence with the highest knockdown efficiency.

Nanocomposite-strengthened dissolving microneedles for improved transdermal delivery to human skin.

Delivery of drugs and biomolecules into skin has significant advantages. To achieve this, herein, a nanomaterial-strengthened dissolving microneedle patch for transdermal delivery is reported. The patch comprises thousands of microneedles, which are composed of dissolving polymers, nanomaterials, and drug/biomolecules in their interior.

Angiogenesis opens a way for Chinese medicine to treat stroke.

Based on the pathophysiology of the brain, advance in angiogenesis induced by stroke, and evidences of Chinese-medicine-mediated angiogenesis, the possibility to study the stroke-treating mechanism of Chinese medicine in angiogenesis was discussed. And regarding our previous work on angiogenesis modulated by qi-tonifying and stasis-eliminating therapy following intracerebral hemorrhage, we proposed some questions, which should be taken into account in the further work.

Treatment of d-galactose induced mouse aging with Lycium barbarum polysaccharides and its mechanism study.

We evaluated the effects of Lycium barbarum polysaccharides LBP) on D-galactose aging model mouse, and explored its possible mechanism. Kunming mice were randomly divided into the control group, the model group, the high-dose LBP group, and the low-dose LBP group. Except the control group, D-galactose was used for modelling.

PEITC reverse multi-drug resistance of human gastric cancer SGC7901/DDP cell line.

Gastric cancer is one of the leading causes of cancer death in the world and nearly all patients who respond initially to cisplatin later develop drug resistance, indicating multi-drug resistance is an essential aspect of the failure of treatment. Phenethyl isothiocyanate (PEITC) has been implicated in inhibiting metastasis of several types of human cancer. However, the effect and potential mechanism of PEITC reversed multi-drug resistance of human gastric cancer is not fully clear.

Electrochemical characteristics of discrete, uniform, and monodispersed hollow mesoporous carbon spheres in double-layered supercapacitors.

Core-shell-structured mesoporous silica spheres were prepared by using n-octadecyltrimethoxysilane (C18TMS) as the surfactant. Hollow mesoporous carbon spheres with controllable diameters were fabricated from core-shell-structured mesoporous silica sphere templates by chemical vapor deposition (CVD). By controlling the thickness of the silica shell, hollow carbon spheres (HCSs) with different diameters can be obtained.

A key time point for cell growth and magnetosome synthesis of Magnetospirillum gryphiswaldense based on real-time analysis of physiological factors.

Pure culture of magnetotactic bacteria with high magnetosome yield has been achieved for only a few strains. The major obstacles involve the nutritional requirements and culture conditions of the cells. To increase cell density and magnetosome production, it is necessary to elucidate the physiological characteristics of a particular strain during cell growth and develop an appropriate artificial control strategy.

Residues of cyantraniliprole and its metabolite J9Z38 in rice field ecosystem.

A simple and reliable analytical method was developed to detect cyantraniliprole (HGW86) and its metabolite J9Z38 in rice straw, paddy water, brown rice, and paddy soil. The fate of cyantraniliprole and its metabolite J9Z38 in rice field ecosystem was also studied. The target compounds were extracted using acetonitrile, cleaned up on silicagel or strong anion exchange column, and analyzed by ultra-performance liquid chromatography-tandem mass spectrometry.

19q13-ERCC1 gene copy number increase in non--small-cell lung cancer.

Background: Excision repair cross complementing 1 gene expression level has potential as a prognostic and predictive marker of the efficacy of chemotherapy in NSCLC. The effect of ERCC1 gene copy number (CN) variation (CNV) on ERCC1 expression and the clinical outcome of patients with NSCLC are not known.

Materials And Methods: Copy number variation of the 19q13.

Prevention of TGFβ induction attenuates angII-stimulated vascular biglycan and atherosclerosis in Ldlr-/- mice.

Angiotensin II (angII) accelerates atherosclerosis, but the mechanisms are not fully understood. The aim of this study was to determine whether TGFβ is required for angII-induced atherosclerosis. Ldlr-null mice fed a normal chow diet were infused with angII or saline for 28 days.

Electroacupuncture at Zusanli (ST36) accelerates intracerebral hemorrhage-induced angiogenesis in rats.

Objective: To investigate the effects of electro-acupuncture on intracerebral hemorrhage (ICH)-induced angiogenesis and hypoxia-inducible factor-1α (HIF-1α) expression in rats.

Methods: Adult male Sprague-Dawley rats were randomly divided into 4 groups of 24 rats each. ICH was induced in 3 groups by stereotactic injection of collagenase type VII into the right globus pallidus; of these, one group was not further treated, the second group underwent Zusanli (ST36)-acupuncture, and the third group underwent non-acupoint acupuncture.

Cloning and characterisation of NADPH-dependent cytochrome P450 reductase gene in the cotton bollworm, Helicoverpa armigera.

Background: Previous studies in our laboratory showed that cytochrome P450 CYP6B7 plays a critical role in a Handan fenvalerate resistant strain (HDFR) of Helicoverpa armigera. As an important component of P450 enzyme systems, cytochrome P450 reductase (CPR) plays an essential role in transferring electrons from NADPH to the P450-substrate complex. However, little information about CPR in H.

MicroRNA-17-92 cluster mediates the proliferation and survival of neural progenitor cells after stroke.

The role of microRNAs (miRNAs) in mediating adult neurogenesis after stroke has not been extensively studied. The present study investigated the function of the miR17-92 cluster in adult neural progenitor cells after experimental stroke. We found that stroke substantially up-regulated miR17-92 cluster expression in neural progenitor cells of the adult mouse.

[Spatial distribution of inhalable particulate and association with respiratory disease in Beijing City].

Inhalable particulate has become the premier pollutant of Beijing, which has enormous influence on the environmental quality of the city and health of the residents. Inhalable particle pollutants (particulate matter of 0.3, 0.

Facile synthesis of hollow silica spheres with nanoholes.

The synthesis of designed nanostructures is an ultimate target of nanomaterial science. Here, silica spheres with nanoholes have been selectively prepared. Coating the necklace-like structured and magnetic nanoparticles along carbon nanotubes, which is an essential step of the simple synthetic procedure of silica hollow spheres, led to the formation of silica hollow spheres with controllable sized nanoholes in their shell walls.

MicroRNA-182 plays an onco-miRNA role in cervical cancer.

Objectives: The purposes of this study were to identify aberrantly expressed miRNAs and investigate their pathogenic roles in cervical cancer.

Methods: miRNA expression was assessed in cervical cancer cell lines, micro-dissected normal cervical epithelium cells and primary cervical carcinoma by TaqMan RT-PCR. Spatial expression of miR-182 in cervical carcinoma and normal cervix was explored by in situ hybridization.

Near-infrared responsive conjugated polymers to 1.5 μm and beyond: synthesis and electrochromic switching application.

Azulene-containing conjugated polymers with near-infrared absorption up to 1.5 μm and beyond are achieved by treating with trifluoroacetic acid (TFA). Density functional theory calculations reveal that the near-infrared absorption arises from a strong intramolecular charge transfer transition on the polymer backbone, and the near-infrared absorption can be tuned by the degree of protonation.

miR-214 targets ATF4 to inhibit bone formation.

Emerging evidence indicates that microRNAs (miRNAs) have important roles in regulating osteogenic differentiation and bone formation. Thus far, no study has established the pathophysiological role for miRNAs identified in human osteoporotic bone specimens. Here we found that elevated miR-214 levels correlated with a lower degree of bone formation in bone specimens from aged patients with fractures.

Controlled chain-scission of polybutadiene by the Schwartz hydrozirconation.

Controlled chain-scission of polybutadiene (PB), polyisoprene, and poly(styrene-co-butadiene), induced by bis(cyclopentadienyl) zirconium hydrochloride (Cp(2)ZrHCl), was revealed at room temperature. The chain-scission reaction of linear PB was studied by means of GPC, NMR spectroscopy, and MALDI-TOF-MS. It was confirmed that the molecular weights of degraded products were quasi-quantitatively controlled by Cp(2)ZrHCl loading, irrespective of the starting PB, whereas the microstructure of PB chains was crucial to the scission reaction.