A rapid and validated GC-MS/MS method for simultaneous quantification of serum Myo- and D-chiro-inositol isomers.

Background: Myo-inositol (MI) and D-chiro-inositol (DCI) are two paramount isomers of inositol, both vital in glucose and steroid metabolism. Deficits in MI, DCI or MI/DCI ratio are expressly concerned with several pathological process, whereas MI and DCI lack practical measurement for human specimen.

Methods: To quantify MI and DCI in serum samples simultaneously, a gas chromatography tandem mass spectrometry (GC-MS/MS) method was established.

Screening and Identification of Croton Fruit Poisoning in Food Using GC-MS and HPLC-MS/MS.

Croton fruit poisoning was successfully identified through a series of chromatographic technologies. A suspected compound named 2-methyl-butenoic acid was screened out using gas chromatography-mass spectrometry, and the hydrolysate of isoguanosine in soup sample was further detected by high performance liquid chromatography-tandem mass spectrometry. The identification of croton fruit poisoning was confirmed basing on these laboratory tests together with the clinical symptoms of the patients.

[Composition and analysis of fatty acids in freshwater products in Hunan Province in 2014].

Objective: To investigate the nutritional composition of fatty acids in freshwater products in Hunan Province.

Methods: The edible parts of freshwater products were detected by gas chromatography, and fatty acid fingerprints were obtained by statistical analysis.

Results: A total of 18 freshwater products were monitored and 14-26 fatty acids were detected in each freshwater product.

[Determination of lipase activity by gas chromatography].

A rapid gas chromatography method was developed for determination of lipase activity using tributyrin as substrate. The standard curves of butyric acid hydrolyzed from tributyrin were linear in the range of 0.11-11.