Functional investigation of the Val1714Gly and Asp1733Gly variants by computational tools and yeast transcription activation assay.

Mutations in the gene are known to be a major cause of hereditary breast cancer. However, characterizing the point associated with cancer in is challenging because the functional impact of most of them is still unknown. Nowadays, a variety of methods are employed to identify cancer-associated mutations in .

Exosomes in Cancer Liquid Biopsy: A Focus on Breast Cancer.

The important challenge about cancer is diagnosis in primary stages and proper treatment. Although classical clinico-pathological features of the tumor have major prognostic value, the advances in diagnosis and treatment are indebted to discovery of molecular biomarkers and control of cancer in the pre-invasive state. Moreover, the efficiency of available therapeutic options is highly diminished, and chemotherapy is still the main treatment due to lack of enough specific targets.

Clinical significance of NDRG3 in patients with breast cancer.

Aim: The expression level of NDRG3 gene is investigated among breast cancer (BC) patients.

Methods: Real-time quantitative PCR was performed.

Results:  NDRG3 was downregulated in BC patients particularly in advanced stage of the disease.

Expression of granulocyte colony stimulating factor (GCSF) in Hansenula polymorpha.

Background And Objectives: During past decades Hansenula polymorpha has attracted global attention for the expression of recombinant proteins due to its high growth rate, minimal nutritional porequirements and use of methanol as a low cost inducer.

Materials And Methods: The corresponding nucleotide sequences for the expression of heterologous genes in Hansenula poylmorpha were extracted and assembled in an E. coli vector.