Publications by authors named "Tanin-Ek Sriwitool"
High surfactant-tolerant β-mannanase isolated from Dynastes hercules larvae excrement, and identification of its hotspot using site-directed mutagenesis and molecular dynamics simulations.
Sitipon Leerawatthanakun Thanapon Charoenwongpaiboon Methus Klaewkla Surasak Chunsrivirot Jitnapa Sirirak Tanin-Ek Sriwitool
Enzyme Microb Technol
March 2022
Article Synopsis
- A β-mannanase (Man26HM7) from Bacillus subtilis HM7 was cloned and expressed in E. coli, showing optimal activity at pH 6.0 and 50 °C.
- This enzyme exhibits strong surfactant stability, maintaining 70% activity in SDS and over 90% in Triton X-100 and Tween 80.
- Research indicates residue 238 is key for SDS tolerance, and modifications to this site in a similar enzyme improved its stability, pointing to potential applications in detergents and enzyme engineering for better surfactant performance.
O-GlcNAcylation, a single attachment of N-acetylglucosamine (GlcNAc) on serine and threonine residues, plays important roles in normal and pathobiological states of many diseases. Aberrant expression of O-GlcNAc modification was found in many types of cancer including colorectal cancer (CRC). This modification mainly occurs in nuclear-cytoplasmic proteins; however, it can exist in some extracellular and secretory proteins.
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