Publications by authors named "Tang Shuo"

Protein lactylation is an emerging field. To advance the exploration of its biological functions, here we develop a comprehensive workflow that integrates proteomics to identify lactylated sites, genetic code expansion (GCE) for the expression of site-specifically lactylated proteins in living cells, and an integrated functional analysis (IFA) platform to evaluate their biological effects. Using a combined wet-and-dry-lab proteomics strategy, we identify a conserved lactylation at ALDOA-K147, which we hypothesize plays a significant biological role.

View Article and Find Full Text PDF

Melanoma, recognized as one of the most aggressive forms of skin cancer, continues to show a steady rise in global incidence. While Bacillus Calmette-Guérin (BCG) has been identified as a potential intralesional therapy for melanoma, its therapeutic efficacy remains suboptimal. This study introduces a novel thermosensitive hydrogel formulated with BCG lysates and either OVA peptide or tumor cell lysates (PPP-BCG-OVA/TL).

View Article and Find Full Text PDF
Article Synopsis
  • Researchers developed a method using Rh(III) catalysts to create fluorescent peptides by activating C-H bonds in Phe-based peptides with alkynes.
  • The process showcases a wide range of substrates, efficient use of materials, and precision in target selection.
  • The resulting fluorescent compounds have strong emission properties and low toxicity, making them suitable for applications in biological studies, specifically targeting lysosomes and mitochondria in live cells.
View Article and Find Full Text PDF
Article Synopsis
  • The study developed a chitosan-based composite sponge (SF/CS/K) using carboxylated sisal fiber and kaolin to enhance hemostatic properties, employing a freeze-drying method with various weight ratios.
  • * The composite sponges exhibited significant qualities like high mechanical strength, porosity, biodegradation, and effective antibacterial activity, making them suitable for medical applications.
  • * In tests involving rat models, the SF/CS/K sponge showed superior coagulation and hemostatic effects compared to commercial sponges, indicating its potential as a novel solution for treating severe bleeding.
View Article and Find Full Text PDF

Lignin is the most abundant natural aromatic polymer in the world. Currently, researchers have developed a number of lignin-based composite materials that are widely used in various fields, including industry, agriculture and medicine. Especially in recent years, lignin has attracted great interest as a high-value product for biomedical applications.

View Article and Find Full Text PDF

Hybrid physics-based data-driven models, namely, augmented physics-based models (APBMs), are capable of learning complex state dynamics while maintaining some level of model interpretability that can be controlled through appropriate regularizations of the data-driven component. In this article, we extend the APBM formulation for high-order Markov models, where the state space is further augmented with past states (AG-APBM). Typically, state augmentation is a powerful method for state estimation for a high-order Markov model, but it requires the exact knowledge of the system dynamics.

View Article and Find Full Text PDF

To explore the impacts of galactose side-chain on the prebiotic activity of xyloglucan oligosaccharides (XGOS), XGOS and de-galactosylated XGOS (DG-XGOS) were prepared from tamarind using an enzymatic method. The differences in structural features of XGOS and DG-XGOS were systematically analyzed. Their fermentation characteristics of human fecal microbiota were explored.

View Article and Find Full Text PDF
Article Synopsis
  • Lysine lactylation is a newly identified modification that plays a significant role in various biological processes including immunity, cancer, and neurodegenerative diseases.
  • The Genetic Code Expansion (GCE) technique is utilized to incorporate lactyllysine (Klac) into proteins, but its effectiveness is hindered by Klac's poor cellular uptake due to high hydrophilicity.
  • To improve this uptake, researchers developed esterified Klac derivatives like KlacOEt, which significantly boosted the expression of lactylated proteins and revealed important insights into how lactylation affects protein function and activity.
View Article and Find Full Text PDF

The arabinogalactan in the representative softwood biomass of larch was degraded using an environmentally friendly hydrogen peroxide and vitamin C (HO-V) system to improve its immunomodulatory activity. Through the HO-V degradation mechanism, hydroxyl radicals are generated, which then target the hydrogen atoms within polysaccharides, resulting in the breaking of glycosidic bonds. Given the impact of oxidative degradation on polysaccharides, we identified three specific arabinogalactan degradation products distinguished by their arabinosyl side chain compositions.

View Article and Find Full Text PDF

Background: Hyaluronic acid (HA) injection in the auricular base is one of the most popular and non-surgical cosmetic procedures for correcting lying ears and optimizing the facial profile because of its minimal invasiveness, immediate effect and safety (Li et al. in Aesthet Surg J 44: 746-75, 2024). But we have recently discovered that this treatment may lead to a new and rare complication called peripheral facial paralysis that has never been reported before.

View Article and Find Full Text PDF

Nano-hydroxyapatite (n-HA) is the main inorganic component of natural bone, which has been widely used as a reinforcing filler for polymers in bone materials, and it can promote cell adhesion, proliferation, and differentiation. It can also produce interactions between cells and material surfaces through selective protein adsorption and has therefore always been a research hotspot in orthopedic materials. However, n-HA nano-particles are inherently easy to agglomerate and difficult to disperse evenly in the polymer.

View Article and Find Full Text PDF

Genetically encoding proximal-reactive unnatural amino acids (PrUaas), such as fluorosulfate-l-tyrosine (FSY), into natural proteins of interest (POI) confer the POI with the ability to covalently bind to its interacting proteins (IPs). The PrUaa-incorporated POIs hold promise for blocking undesirable POI-IP interactions. Selecting appropriate PrUaa anchor sites is crucial, but it remains challenging with the current methodology, which heavily relies on crystallography to identify the proximal residues between the POIs and the IPs for the PrUaa anchorage.

View Article and Find Full Text PDF

In the process of the intelligent inspection of belt conveyor systems, due to problems such as its long duration, the large number of rollers, and the complex working environment, fault diagnosis by acoustic signals is easily affected by signal coupling interference, which poses a great challenge to selecting denoising methods of signal preprocessing. This paper proposes a novel wavelet threshold denoising algorithm by integrating a new biparameter and trisegment threshold function. Firstly, we elaborate on the mutual influence and optimization process of two adjustment parameters and three wavelet coefficient processing intervals in the BT-WTD (the biparameter and trisegment of wavelet threshold denoising, BT-WTD) denoising model.

View Article and Find Full Text PDF

The editors introduce the feature issue on "Novel Techniques in Microscopy," which was the topic of a symposium held on April 24-27, 2023, in Vancouver, BC. This symposium was part of the Optics in the Life Sciences Congress.

View Article and Find Full Text PDF

Algae plays a key role in carbon capture and utilization (CCU) as it can capture and use the atmospheric CO for conversion of value-added products. Concentrated CO is common in flue gas and provides opportunities for algae cultivation. The drawbacks are mass transfer limitation, poor CO dissolution, and challenges to reach optimal levels for algal growth at given flue gas levels.

View Article and Find Full Text PDF

The innovation in recombinant protein technology has brought forth a host of challenges related to the purification of these therapeutic proteins. This article delves into the intricate landscape of developing purification processes for artificially designed therapeutic proteins. The key hurdles include controlling protein reduction, protein capture, ensuring stability, eliminating aggregates, removing host cell proteins and optimizing protein recovery.

View Article and Find Full Text PDF
Article Synopsis
  • * This study introduces a multi-level segmentation network comprising modules for data quality enhancement, coarse and fine segmentation, and localization, with a novel W-Net and boundary loss in the fine segmentation stage.
  • * The proposed model demonstrated superior optic disc segmentation performance across multiple datasets, outperforming existing state-of-the-art methods.
View Article and Find Full Text PDF

We report on a compact multimodal imaging system that can acquire two-photon microscopy (2PM) and three-photon microscopy (3PM) images simultaneously. With dual excitation wavelengths, multiple contrasts including two-photon-excitation-fluorescence (2PEF), second harmonic generation (SHG), and third harmonic generation (THG) are acquired simultaneously from cells, collagen fibers, and interfaces, all label-free. Challenges related to the excitation by two wavelengths and the effective separation of 2PM and 3PM signals are discussed and addressed.

View Article and Find Full Text PDF

Irreversible eye lesions, such as glaucoma and traumatic optic neuropathy, can cause blindness; however, no effective treatments exist. The optic nerve, in particular, lacks the capacity to spontaneously regenerate, requiring the development of an effective approach for optic nerve repair, which has proven challenging. Here, we demonstrate that a combination of the small molecules 3BDO and trichostatin A (TSA)-which regulate mTOR and HDAC, respectively-packaged in thermosensitive hydrogel for 4-week-sustained release after intravitreal injection, effectively induced optic nerve regeneration in a mouse model of optic nerve crush injury.

View Article and Find Full Text PDF

A novel co-hybrid nano-apatite (n-HA) by introducing lignin derivatives (LDs) and alendronate (ALE) was designed to reinforce poly(lactide-co-glycolide) (PLGA). The effect of different addition methods and contents of LDs, lignin derivatives sorts of lignosulfonate (LS), alkali lignin (AL) and carboxymethyl lignin (CML), and the addition order of ALE on the dispersion of hybrid n-HA, and reinforce effective for PLGA were investigated by FTIR, XRD, TEM, TGA, XPS, N adsorption/desorption, zeta potential, dispersion experiments, universal testing machine, SEM, DSC and POM. The results showed that the addition order could regulate the growth of n-HA crystal planes by binding with Ca, and co-hybrid HA by LDs and ALE possessed better dispersion owing to the synergistic effect.

View Article and Find Full Text PDF

Background: Zhu-Tokita-Takenouchi-Kim (ZTTK, OMIM 617140) syndrome is a severe multisystem developmental disorder characterized by intellectual disability, developmental delay, cortical malformations, epilepsy, visual problems, musculoskeletal abnormalities, and congenital malformations. ZTTK syndrome is caused by a heterozygous pathogenic variant of the SON gene (NM_138927) at chromosome 21q22.1.

View Article and Find Full Text PDF
Article Synopsis
  • This study explores the development of an ideal guided bone regeneration (GBR) membrane using tragacanth gum (GT) combined with chitosan and strontium-doped nano-hydroxyapatite (Sr-HA).
  • The research investigates how various component ratios and the inclusion of Sr-HA affect the physical and chemical properties of the resulting CS/Sr-n-HA/GT composite membrane.
  • Findings indicate that the optimal composition (CS/Sr-n-HA/GT ratio of 6:2:2) resulted in enhanced ionic crosslinking, mechanical strength, and biocompatibility, suggesting potential for improved applications in biomedical membranes.
View Article and Find Full Text PDF

A proximity-enabled protein cross-linking strategy with additional spatiotemporal control is highly desirable. Here, we report an oxidation-induced protein cross-linking strategy involving the incorporation of a vinyl thioether group into proteins in both and mammalian cells via genetic code expansion. We demonstrated that vinyl thioether can be selectively induced by exogenously added oxidant or by reactive oxygen species from the cellular environment, as well as by photocatalysts, and converted into a Michael acceptor, enabling fluorescence labeling and protein cross-linking.

View Article and Find Full Text PDF

Bone injury caused changes to surrounding tissues, leading to a large number of osteoclasts appeared to clear the damaged bone tissue before bone regeneration. However, overactive osteoclasts will inhibit bone formation. In this study, we prepared methacrylylated gelatin (GelMA)-based hydrogel to co-crosslink with OP3-4 peptide, a receptor activator of NF-κB ligand (RANKL) binding agent, to achieve the slow release of OP3-4 peptide to inhibit the activation of osteoclasts, thus preventing the long-term existence of osteoclasts from affecting bone regeneration, and promoting osteogenic differentiation.

View Article and Find Full Text PDF

The copolymerization of carbon dioxide (CO) with epoxides demonstrates promise as a new synthetic method for low-carbon polymer materials, such as aliphatic polycarbonate materials. In this study, a binary Schiff base cobalt system was successfully used to catalyze the copolymerization of 1,2-butylene oxide (BO) and CO and its terpolymerization with other epoxides such as propylene oxide (PO) and cyclohexene oxide (CHO). H nuclear magnetic resonance (H NMR), diffusion-ordered spectroscopy (DOSY), gel permeation chromatography (GPC), and differential scanning calorimetry (DSC) confirmed the successful synthesis of the alternating terpolymer.

View Article and Find Full Text PDF