Individuals at risk of beta-blocker discontinuation: a cohort study in 19,177 Chinese patients.

Purpose: This cohort study evaluated the factors associated with discontinuation of antihypertensive pharmacotherapy among Chinese patients who were prescribed beta-blockers. We tested the hypothesis that patients' age, gender, socioeconomic status, clinical settings and the number of comorbidities were significantly associated with the discontinuation of beta-blockers.

Methods: From a validated clinical database we included all adult patients 18 years or older who were prescribed a beta-blocker in any government primary care clinic in one large territory of Hong Kong during 01 January 2004-30 June 2007.

Mitotic arrest defective protein 2 expression abnormality and its clinicopathologic significance in human osteosarcoma.

Osteosarcoma is the most common primary malignancy of bone. Overexpression of mitotic arrest defective protein 2 (MAD2) is found in many human neoplasms, but its role in the oncogenesis of osteosarcoma is an untouched topic. The objective of this research was to observe the expression of MAD2 in human osteosarcoma and explore its clinicopathologic significance.

Molecular events of cellular apoptosis and proliferation in the early tendon healing period.

Purpose: Cellular proliferation is accompanied by cellular apoptosis. In the healing digital flexor tendon, molecular events concerning cellular apoptosis have not been investigated. This study aimed to investigate the relationship between cellular apoptosis and proliferation in early tendon healing.

Effectiveness of microRNA in Down-regulation of TGF-beta gene expression in digital flexor tendons of chickens: in vitro and in vivo study.

Purpose: Transforming growth factor (TGF)-beta is considered to be responsible for the formation of scars such as adhesions around healing digital flexor tendons. We proposed to deliver microRNAs (miRNAs) to silence expression of the TGF-beta1 gene and to investigate the effectiveness of miRNAs in down-regulation of the TGF-beta1 gene in vitro and in vivo.

Methods: We designed and engineered 4 miRNAs according to genetic sequences of chicken TGF-beta1.

Strength of tendon repair decreases in the presence of an intact A2 pulley: biomechanical study in a chicken model.

Purpose: Pulleys have an important role in digital flexion, but little is known about how the strength of a tendon repair is affected by a major pulley. We evaluated the difference in strengths of flexor tendon repairs in the pulley area when the pulley was intact or divided in a chicken tendon injury model.

Methods: In each of 100 long toes of 50 Leghorn chickens, a complete, transverse laceration of the flexor digitorum profundus tendon was made in the region of the A2 pulley and was repaired surgically.

Effect of A2 pulley release on repaired tendon gliding resistance and rupture in a chicken model.

Purpose: Release of part of the A2 pulley has been suggested to improve function after zone II primary flexor tendon repairs. The purpose of this study was to measure the effect of A2 pulley release compared with pulley preservation on repaired tendon gliding resistance and rupture in the early postoperative period in a chicken model.

Methods: We divided 104 long toes from 52 Leghorn chickens into 2 experimental groups.

Fluid resuscitation for major burn patients with the TMMU protocol.

Background: Fluid resuscitation is one of the critical treatments for the major burn patient in the early phases after injury. We evaluated the practice of fluid resuscitation for severely burned patients with the Third Military Medical University (TMMU) protocol, which is most widely used in many regions of China.

Methods: Patients with major burns (>30% total body surface area (TBSA)) presenting to Southwest Hospital, Third Military Medical University, between January 2005 and October 2007, were included in this study.

Endogenous tissue plasminogen activator increases hemorrhagic transformation induced by heparin after ischemia reperfusion in rat brains.

Objective: Tissue plasminogen activator (tPA) as a main thrombolytic drug for acute ischemic stroke remains complicated by risk of hemorrhagic transformation. However, whether endogenous tPA is also involved in hemorrhagic transformation is yet unclear.

Methods: We randomly assigned male Sprague-Dawley rats into three groups: the heparin group, the control group and the sham operated group.

Albumin ameliorates tissue plasminogen activator-mediated blood-brain barrier permeability and ischemic brain injury in rats.

Objective: Tissue plasminogen activator (tPA) may aggravate ischemic neuronal damage after focal cerebral ischemia and increase blood-brain barrier permeability. Human serum albumin has neuroprotective effects on ischemic stroke. However, whether albumin can attenuate the deleterious effects of tPA is yet unknown.

In vivo length changes of selected carpal ligaments during wrist radioulnar deviation.

Purpose: To investigate changes in the lengths of selected carpal ligaments during wrist radioulnar deviation in vivo.

Methods: We studied in vivo changes in the lengths of fibers of 5 palmar and dorsal intracapsular ligaments of the wrist during radioulnar deviation in 6 wrists of healthy volunteers using a noninvasive approach. Using serial computed tomography scans and volume registration techniques, the carpal kinematics were examined at 4 positions, from 40 degrees ulnar deviation to 20 degrees radial deviation, in 20 degrees increments.

Application of AAV2-mediated bFGF gene therapy on survival of ischemic flaps: effects of timing of gene transfer.

Necrosis of surgically transferred flaps is a major problem in reconstructive surgery. We investigated efficacy of a new vector system-adeno-associated viral 2 (AAV2)-mediated bFGF gene transfer to enhance survival of the ischemic flap. Thirty-eight Sprague-Dawley rats were divided into 3 gene therapy groups and 1 nontreated control of 9 or 10 each.

Antibacterial effect of dressings containing multivalent silver ion carried by zirconium phosphate on experimental rat burn wounds.

To evaluate the antibacterial effect of multivalent silver ion carried by zirconium phosphate (ZP-Ag) both in vitro and in an experimental rat burn wound compared with that of sulfadiazine silver (SD-Ag). Firstly, the minimal inhibition concentration and the minimal bactericidal concentration of ZP-Ag to three different strains of bacteria (Staphylococcus aureus, Pseudomonas aeruginosa, and Escherichia coli) were compared with those of SD-Ag in vitro. Following this dressings containing the same mass of ZP-Ag or SD-Ag were applied to wounds in a rat burn model and their antimicrobial activity assayed.

Advances in multimodality imaging through a hybrid PET/MRI system.

The development of integrated imaging systems for magnetic resonance imaging (MRI) and positron emission tomography (PET) is currently being explored in a number of laboratories and industrial settings. PET/MRI scanners for both preclinical and human research applications are being developed. PET/MRI overcomes many limitations of PET/computed tomography (CT), such as limited tissue contrast and high radiation doses delivered to the patient or the animal being studied.

Tendon healing in vivo: gene expression and production of multiple growth factors in early tendon healing period.

Purpose: The actions of growth factors during healing of injured flexor tendons are not well characterized, although information pertinent to some individual growth factors is available. We studied gene expression and protein production of a number of growth factors at several time points during the early healing period in a chicken model.

Methods: Seventy-four long toes of 37 white Leghorn chickens were used.

In vivo changes in lengths of the ligaments stabilizing the distal radioulnar joint.

Purpose: To investigate changes in lengths of the ligaments stabilizing the distal radioulnar joint during forearm pronation and supination in vivo.

Methods: We studied in vivo kinematics of the distal radioulnar joint by measuring the length changes of the ligaments of the distal radioulnar joint in 6 wrists of normal volunteers. Using serial computed tomography scans and volume registration techniques, the distal radioulnar joints were examined at 7 positions from 90 degrees pronation to 90 degrees supination in 30 degrees increments.

Femoral head epiphysis growth and development among Chinese children aged 0-5 years.

The aim of this study was to examine the pattern of femoral head epiphysis growth and development among Chinese children. Between January and December, 2007, we randomly sampled 1,450 healthy Chinese children (0-5 years old) from Hunan Provincial Children's Hospital in Changsha, Hunan, China. The diameter of femoral head epiphysis was measured by pelvic X-ray photography and processed by medical image processing software.

A simple and efficient method for deriving neurospheres from bone marrow stromal cells.

Bone marrow stromal cells (MSCs) can be differentiated into neuronal and glial-like cell types under appropriate experimental conditions. However, previously reported methods are complicated and involve the use of toxic reagents. Here, we present a simplified and nontoxic method for efficient conversion of rat MSCs into neurospheres that express the neuroectodermal marker nestin.

Adeno-associated virus-2-mediated bFGF gene transfer to digital flexor tendons significantly increases healing strength. an in vivo study.

Background: Treatment of the disrupted intrasynovial flexor tendon is troublesome and can be complicated by the rupture of weak repairs and the formation of adhesions. The central issue underlying the unsatisfactory outcomes is the lack of sufficient healing capacity, which prohibits aggressive postoperative tendon motion. Transfer of genes that are critical to healing by means of an efficient vector system offers a promising way of strengthening the repair.

Prevalence of plasmid-mediated AmpC beta-lactamases in a Chinese university hospital from 2003 to 2005: first report of CMY-2-Type AmpC beta-lactamase resistance in China.

The aim of this study was to investigate the prevalences of plasmid-mediated AmpC beta-lactamases (PABLs) in isolates of Escherichia coli and Klebsiella spp. from a university hospital in China. A total of 1,935 consecutive nonrepeat clinical isolates of Escherichia coli, Klebsiella pneumoniae, and Klebsiella oxytoca were collected between January 2003 and July 2005.

Increased plasma acylation-stimulating protein in pediatric proteinuric renal disease.

Hyperlipidemia has been well recognized as a striking feature of nephrotic syndrome and other renal diseases. However, the underlying pathophysiological mechanisms still have not yet been elucidated. In this study, we evaluated acylation-stimulating protein (ASP) and complement component 3 (C3) in children (n=48) with various forms of proteinuric renal disease [nephrotic syndrome, acute poststreptococcal infection glomerulonephritis (APSGN), and lupus nephritis (LN)] in comparison with age- and gender-matched controls (n=279).