Systematic identification of R2R3-MYB S6 subfamily genes in Brassicaceae and its role in anthocyanin biosynthesis in Brassica crops.

The Brassicaceae family includes Arabidopsis thaliana, various vegetables and oil crops. The R2R3-MYB genes of the S6 subfamily are crucial for regulating anthocyanin biosynthesis, however, their systematic identification in Brassicaceae plants is still incomplete. Here, we systematically identified homologous genes of R2R3-MYB transcription factors from the S6 subfamily across 31 Brassicaceae species.

Molecular insights into sperm head shaping and its role in human male fertility.

Background: Sperm head shaping, controlled by the acrosome-acroplaxome-manchette complex, represents a significant morphological change during spermiogenesis and involves numerous proteins expressed in a spatially and temporally specific manner. Defects in sperm head shaping frequently lead to teratozoospermia concomitant with oligozoospermia and asthenozoospermia, but the pathogenic mechanism underlying sperm head shaping, and its role in male infertility, remain poorly understood.

Objective And Rationale: This review aims to summarize the mechanism underlying sperm head shaping, reveal the relationship between gene defects associated with sperm head shaping and male infertility in humans and mice, and explore potential clinical improvements in ICSI treatment.

Repurposing fenticonazole nitrate to restore colistin susceptibility in multidrug-resistant bacteria.

Aims: To explore the synergistic effect of the combination of FN and colistin on Escherichia coli and further elucidate the mechanism of this effect.

Main Methods: Antimicrobial efficacy of the combination of fenticonazole nitrate and colistin against Escherichia coli in vitro using MIC assays, checkerboard assays, growth curves, and time-kill curves. Crystalline violet staining for detection of biofilm.

MicroRNA-106a regulates the apoptosis and oxidative stress of porcine trabecular meshwork cells by targeting FAS.

This study investigates the impact of miR-106a on trabecular meshwork (TM) and its potential molecular mechanism, as TM dysfunction due to decreased cell viability is a major pathological feature of POAG. Primary porcine TM (PTM) cells were isolated and exposed to hyperoxic conditions to induce senescence. Through small RNA sequencing and qPCR verification, miR-106a was downregulated in aging PTM cells.

T-2 toxin-induced splenic injury by disrupting the gut microbiota-spleen axis via promoting IL-6/JAK/STAT1 signaling-mediated inflammation and apoptosis and its mitigation by elemental nano-selenium.

T-2 toxin is one of the most toxic A trichothecene mycotoxins prevalent in the environment and food chain, which brings severe health hazards to both animals and humans and it can significantly damage immune function. In this study, we comprehensively explained the impact of T-2 toxin on the spleen through gut microbiota-spleen axis by integrating the transcriptome and microbiome. Our results revealed that dietary T-2 toxin ≥ 1.

Minimally Invasive and Navigation-Assisted Fracture Stabilization Following Traumatic Spinopelvic Dissociation.

Spinopelvic dissociation is a highly unstable orthopedic injury with a growing incidence worldwide. Operative treatment classically involves an open lumbopelvic fusion and sacroiliac stabilization, which carries high perioperative morbidity and mortality in a frail patient population. Advancements in spinal navigation, robotics, and minimally invasive surgery (MIS) techniques now allow these fracture patterns to be treated entirely percutaneously through small incisions.

Inverse relationship between circulating sphingosine-1-phosphate and precursor species and coronary artery calcification score in type 2 diabetes.

Background: Sphingosine 1-phosphate (S1P) is a key mediator of lipid signaling with strong immunomodulatory and anti-inflammatory effects. Circulating S1P levels including S1P in high-density lipoproteins (HDL) were demonstrated to be inversely associated with cardiovascular diseases (CVD). However, no studies are available regarding a potential implication of S1P on the risk of CVD in type 2 diabetes (T2D).

[Development Path of Guangxi to Reach the Carbon Emission Peak Based on STIRPAT Model].

In this work, to ensure that Western China achieves the goal of peaking carbon emissions while developing, considering Guangxi as an example, based on the statistical data of population, economy, society, energy, urban development, and other dimensions from 2000 to 2021, the total amount of carbon emissions and sinks from 2000 to 2021 was calculated according to the recommended formula of the Intergovernmental Panel on Climate Change （IPCC）, the key factors affecting carbon emissions based on the ridge regression were identified, the STIRPAT carbon emission prediction model was constructed, and the carbon emissions of Guangxi from 2022 to 2040 were predicted for the three scenarios of different development paths. The results showed that the total carbon dioxide emissions （CO） in Guangxi showed an overall upward trend from 2000 to 2021, growing from 95.49 Mt in 2000 to 390.

Electroacupuncture at ST36 Relieves Visceral Hypersensitivity Based on the Vagus-Adrenal Axis in the Remission Stage of Ulcerative Colitis.

Background And Aims: Although electroacupuncture (EA) at ST36 has been shown to alleviate visceral hypersensitivity in rats with ulcerative colitis (UC), the exact mechanism remains unknown. This study aims to investigate whether EA can effectively inhibit the activity of enteric glial cells (EGCs) through the adrenergic antiinflammatory pathway and thereby attenuate visceral hypersensitivity in rats with UC in remission.

Materials And Methods: Sprague-Dawley rats were continuously fed 5% dextran sulfate sodium (DSS) for seven days to establish intestinal inflammation.

Remote sensing estimation of water volume changes of typical lakes in Xinjiang, China from 1990 to 2021.

Inland lakes are important surface water resources in arid Central Asia. Due to the superimposed influence of natural factors and human factors, the hydrological characteristics of arid lakes show significant temporal and spatial variations. However, data shortage in this area makes it difficult to carry out detailed and long-term quantitative monitoring of hydrological regimes for different lakes.

Evaluation of GPT-4 concordance with north American spine society guidelines for lumbar fusion surgery.

Background: Concordance with evidence-based medicine (EBM) guidelines is associated with improved clinical outcomes in spine surgery. The North American Spine Society (NASS) has published coverage guidelines on indications for lumbar fusion surgery, with a recent survey demonstrating a 60% concordance rate across its members. GPT-4 is a popular deep learning model that receives knowledge training across public databases including those containing EBM guidelines.

Single-cell transcriptomic profiling of rat iridocorneal angle at perinatal stages: Revisiting the development of periocular mesenchyme.

The periocular mesenchyme (POM) gives rise to key structures in the ocular anterior segment, and its malformation leads to anterior segment dysgenesis (ASD) with iridocorneal angle (ICA) abnormalities. However, the transcriptional profile of the POM and the regulatory mechanisms governing cell-fate decision during anterior eye and ICA development remain poorly understood. In this study, we performed a comprehensive time-series analysis by sequencing rat anterior ocular samples collected at five consecutive perinatal stages: embryonic days 16.

Enteric pathogenicity characterization of emerging parainfluenza virus 5 in western China.

Parainfluenza virus 5 (PIV5) is a member of the Paramyxoviridae family and causes respiratory symptoms in various animal species. Although the virus has been frequently detected among fecal samples, no study has described its infection of the intestine. Recently, diarrhea with low mortality has spread on pig farms in Gansu, China.

Functional Polyolefins and Composites.

Polyolefins are simple hydrocarbons that require additional chemical modifications or functional additives to give them custom functions. Recent research in the development of functional polyolefins has surpassed the traditional approach of simply improving surface properties by incorporating polar moieties. Creating custom functionalized polyolefins by using specific functional units has attracted increasing attention.

Causal effect of C-reaction protein and endometrial cancer: Genetic evidence of the role of inflammation in endometrial cancer.

Consensus remains elusive regarding the relationship between C-reactive protein (CRP) levels and endometrial cancer (EC). Our study sought to elucidate the causal association between CRP and EC, aiming to contribute to the understanding of this complex interplay. We primarily utilized the random-effects inverse variance-weighted method.

Characterization of a highly pathogenic porcine Teschoviruses 5 emerged in Western China.

Porcine teschovirus (PTV) is a devastating virus that targets the central nervous system and led to great economic losses in Europe between the 1920s and 1960s. Since 1973, PTV variants with lower pathogenicity have been prevalent globally, whereas highly pathogenic PTV strains have rarely emerged. In 2022, diarrhea with high mortality occurred on a pig farm in Gansu China.

Dapagliflozin Mediates the Protective Effect against atrial fibrillation/atrial flutter and the Reduction in All-Cause Mortality Risk.

Objective: This study aimed to investigate the association between dapagliflozin and the incidence of atrial fibrillation (AF) and atrial flutter (AFL), along with its impact on all-cause mortality in patients with diabetes mellitus (DM).

Material And Methods: Adhering to the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines, this meta-analysis conducted a comprehensive search across PubMed, Embase, and ClinicalTrials.gov databases up to June 2021.

Molecular Characterization and Pathogenicity Analysis of Porcine Rotavirus A.

Porcine rotavirus A (RVA) is one of the major etiological agents of diarrhea in piglets and constitutes a significant threat to the swine industry. A molecular epidemiological investigation was conducted on 2422 diarrhea samples from Chinese pig farms to enhance our understanding of the molecular epidemiology and evolutionary diversity of RVA. The findings revealed an average RVA positivity rate of 42% (943/2422), and the study included data from 26 provinces, primarily in the eastern, southern and southwestern regions.

Fructooligosaccharides for Relieving Functional Constipation: A Systematic Review and Meta-Analysis of Randomized Controlled Trials.

Fructooligosaccharides (FOS) are prebiotics with great potential to improve constipation. This study set out to investigate the usefulness of consuming FOS as a dietary supplement on bowel movement frequency, stool consistency, abundance of Bifidobacteria, gastrointestinal transit time, and gastrointestinal symptoms through a systematic review and meta-analysis of randomized controlled trials (RCTs). We searched the Cochrane Library, PubMed, EMBASE, and Web of Science databases up to 2 March 2024.

Fine mapping and identification of the bright green leaf gene in Chinese kale ( var. alboglabra).

Introduction: Chinese kale ( var. alboglabra), is an annual herb belonging to the Brassica genus of Cruciferae, and is one of the famous specialty vegetables of southern China. Some varieties show bright green leaf (BGL) traits and have better commerciality.

A mucus layer derived from porcine intestinal organoid air-liquid interface monolayer attenuates swine enteric coronavirus infection by antiviral activity of Muc2.

Background: The mucus layer provides the first defense that keeps the epithelium free from microorganisms. However, the effect of the small intestinal mucus layer on pathogen invasion is still poorly understood, especially for swine enteric coronavirus. To better understand virus‒mucus layer‒intestinal epithelium interactions, here, we developed a porcine intestinal organoid mucus‒monolayer model under air‒liquid interface (ALI) conditions.

Expression profiles of microRNA-mRNA and their potential impact on anthocyanin accumulation in purple petals of Brassica napus.

Rapeseed (Brassica napus L.) possesses substantial economic value as an oil, vegetable, and forage crop, while also exhibiting notable ornamental characteristics. Recent advances in flower colour breeding have significantly enhanced the visual appeal of rapeseed, with anthocyanins identified as the primary contributor to the development of red, purple, and pink flowers.

Potassium molybdate blocks APN-dependent coronavirus entry by degrading receptor via PIK3C3-mediated autophagy.

Swine enteric coronaviruses pose a significant challenge to the global pig industry, inflicting severe diarrhea and high mortality rates among piglets, and resulting in substantial economic losses. In our clinical practice, we observed that the addition of potassium molybdate (PM) to the feed could dramatically reduce diarrhea and diarrhea-related mortality in piglets. However, the underlying mechanisms remain elusive and merit further investigation.

Preparation and application of glucono-δ-lactone-induced gel of transglutaminase cross-linked black bean protein isolate-whey protein isolate: Effect of ultrasound pretreatment.

A glucono-δ-lactone induced gel was prepared using transglutaminase cross-linked black bean protein-whey protein to deliver riboflavin. Ultrasound pretreatment was found to positively affected gels' hardness, water holding capacity and elasticity. The hardness and elasticity of protein gel pretreated by ultrasound at 360 W were the best, and the water holding capacity of protein gel pretreated by ultrasound at 480 W was the best.

The Effects of Model Insoluble Copper Compounds in a Sedimentary Environment on Denitrifying Anaerobic Methane Oxidation (DAMO) Enrichment.

The contribution of denitrifying anaerobic methane oxidation (DAMO) as a methane sink across different habitats, especially those affected by anthropogenic activities, remains unclear. Mining and industrial and domestic use of metals/metal-containing compounds can all cause metal contamination in freshwater ecosystems. Precipitation of metal ions often limits their toxicity to local microorganisms, yet microbial activity may also cause the redissolution of various precipitates.