In Vitro and In Vivo Antitumor Activity of (DC.) S. Arias & Terrazas Endophytic and Extracts Against the Murine Lymphoma L5178Y-R.

Cancer belongs to a group of diseases characterized by uncontrolled cell growth. The search for new effective treatments for cancer has led to the discovery of different molecules from plants, bacteria, and fungi with pharmacological use. Plant endophytic fungi are large producers of metabolites with antitumor properties.

Intra-Phenotypic and -Genotypic Variations of (Bals.) Vuill. Strains Infecting L. Adults.

has potential for biological control. However, its efficacy depends on the strain's geographic location, host susceptibility, and virulence. The present study aimed to evaluate the effectiveness of strain BBPTG4 conidia in controlling adults and its detection via introns profile on exposed mosquito corpses.

Role of the sympathetic nervous system in cancer-associated cachexia and tumor progression in tumor-bearing BALB/c mice.

Background: Adipose and muscle tissue wasting outlines the cachectic process during tumor progression. The sympathetic nervous system (SNS) is known to promote tumor progression and research suggests that it might also contribute to cancer-associated cachexia (CAC) energetic expenditure through fat wasting.

Methods: We sympathectomized L5178Y-R tumor-bearing male BALB/c mice by intraperitoneally administering 6-hydroxydopamine to evaluate morphometric, inflammatory, and molecular indicators of CAC and tumor progression.

Natural Prevalence, Molecular Characteristics, and Biological Activity of (Farlow) Isolated from (J. E. Smith) Larvae in Mexico.

Entomopathogenic fungi have been considered potential biological control agents against the fall armyworm (J. E. Smith), the world's most important pest of maize.

and Combination Modulates IFN-γ, IL-10, and SOCS3 in Rotavirus-Infected Cells.

Rotavirus is the main cause of acute diarrhea in children up to five years of age. In this regard, probiotics are commonly used to treat or prevent gastroenteritis including viral infections. The anti-rotavirus effect of and , by reducing viral infectivity and improving IFN-type I response, has been previously reported.

In Planta Detection of (Ascomycota: Hypocreales) Strains as Endophytes in Bean ( L.).

() is a significant entomopathogenic fungus (EPF) in agriculture as a sprayable biocontrol agent. It has the potential to be established as an endophyte (ENP) in various crops, resulting in beneficial effects for the host plants, including resistance to pest insects and increased growth and yield. However, it is not known whether a strain has such a favorable impact on the plant, since it is a common soil microorganism.

Adult Biocontrol Using Strains and Gum Formulations.

Speare is the only entomopathogenic fungus that has been applied to control the hemipteran Kuwayama. However, the use of available commercial products under field conditions is limited due to conidia's shelf life and short environmental persistence. We have previously reported the citrus psyllid adults' biocontrol potential using strains.

and Improve the IFN Type I-Mediated Antiviral Response in Rotavirus-Infected Cells.

Probiotics are effective to treat or prevent gastrointestinal infections, and microalgae have demonstrated important health-promoting effects and in some cases function as prebiotics. In this regard, the anti-rotavirus effect of and by reducing viral infectivity is well known. However, their effect on immune response against rotavirus has not yet been investigated.

Cytotoxic, Anti-Hemolytic, and Antioxidant Activities of L. (Rutaceae) Extract, Fractions, and Isolated Compounds.

is an herb used to treat various ailments, and its potential cytotoxic effects on different tumor cell lines have been extensively studied. The present study aimed to evaluate the cytotoxic activity of methanol extract (RCME), sub-partitions obtained from solvents of increasing polarity, and major compounds, as well as their hemolytic, anti-hemolytic, and antioxidant potential. The in vitro cytotoxic activity against the human hepatocarcinoma (HEP-G2) and the murine lymphoma cell line (L5178Y-R) was evaluated using the colorimetric 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) reduction assay, whereas selectivity indices (SIs) were determined by comparing cytotoxicity against normal African green monkey kidney cells (VERO) and human peripheral blood mononuclear cells (PBMC).

Biocontrol of (Curculionidae: Scolitinae) Females and Progeny by (Hypocreales: Cordycipitaceae) in a Sawdust Artificial Diet Model.

The ambrosia beetle , recently reported affecting avocado trees in Mexico, represents one of the most widespread insects worldwide. Previous reports have shown that genera members are susceptible to and other entomopathogenic fungus strains. However, their effect on borer beetles' progeny has not been fully investigated.

In Vitro Biological Activity and Lymphoma Cell Growth Inhibition by Selected Mexican Medicinal Plants.

Cancer is a major health problem with significant morbidity and mortality. In addition, plants are a source of metabolites with diverse biological properties, including antitumor potential. In this study, we investigated the in vitro murine lymphoma L5178Y-R cell growth inhibition, human peripheral blood mononuclear cells (PBMC) toxicity and proliferation, and antioxidant, hemolytic, and anti-hemolytic activities of methanol extracts from 15 plants of traditional use in Mexico.

Improved (Hemiptera: Liviidae) Adults Biocontrol in Citrus by (Hypocreales: Ophiocordycipitaceae) Gum-Enhanced Conidia Formulation.

Speare is the only entomopathogenic fungus involved in Kuwayama natural epizootics. The aim of the present study was to evaluate different protein sources as supplements to stimulate growth, improve conidiation on solid culture, and evaluate its produced gum for conidia formulation against adults. INIFAP-Hir-2 strain was grown on agar media enriched with wheat bran, wheat germ, soy, amaranth, quinoa, and pumpkin seed, in addition to oat with wheat bran and/or amaranth.

Effect of Culture Conditions of Endophytic Fungi on Yield and Anticancer and Antioxidant Activities.

Culture conditions affect the production of secondary metabolites in endophytic fungi. Therefore, the aim of the present study was to evaluate the yield and anticancer and antioxidant activity of endophytic fungi extracts from the cactus , under different culture conditions. The strains , , and sp.

Pepper growth promotion and biocontrol against by and formulations.

Background: genus has been used in horticultural crops as a biocontrol agent against insect pests, microbial phytopathogens, and plant growth-promoting bacteria (PGPB), representing an alternative to agrochemicals. In particular, (Bc) and (Bt) have been studied for their fungicidal and insecticidal activities. However, their use as biofertilizer formulations and biocontrol agents against phytopathogenic bacteria is limited.

Domestic spp. Infections with , Household Infestations, and Molecular Identification in Oaxaca, México.

In Latin America, Mexico is the country with the second highest annual estimated number of Chagas disease cases, caused by , due to vector-borne transmission. The state of Oaxaca is the location of the first documented human cases of Chagas disease in Mexico and contained the highest seropositive rate (3.5%) from blood donors.

In Vitro Cytotoxic Activity of Methanol Extracts of Selected Medicinal Plants Traditionally Used in Mexico against Human Hepatocellular Carcinoma.

Medicinal plants are traditionally used in Mexico to treat diseases such as cancer. The present study aimed to evaluate the cytotoxic, antioxidant, and anti-hemolytic activity of 15 plants of ethnopharmacological use in Mexico. For this, plant methanol extracts were prepared by the Soxhlet method, after which their cytotoxic activity was evaluated against human hepatocellular carcinoma (HEP-G2) and monkey kidney epithelial (Vero) cells by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) reduction colorimetric assay.

Increased Attraction and Stability of -Formulated Microgranules for Biocontrol.

Aedes aegypti (Linn.) incidence has increased in recent years, causing human viral diseases such as dengue, which are often fatal. Beauveria bassiana (Bals.

Evaluation of the Fluorescence Polarization Assay for the Diagnosis of Brucellosis in Goat Milk.

The milk ring test is a detection assay for antibodies against Brucella in bovine milk. It has good sensitivity but tends to give false positive results. In this study, we standardized the application of the fluorescence polarization assay (FPA) for the detection of antibodies against B.

Insight into Biological Control Potential of against Asian Citrus Psyllid as a Vector of Citrus Huanglongbing Disease in America.

Studies on Speare are scarce. Among these, some reports have focused on phenotypic identification, based on its morphological structure and morphometric characteristics. This fungus is known to control economically important citrus crop pests.

Entomopathogenic fungi tested in planta on pepper and in field on sorghum, to control commercially important species of aphids.

Given the aphids high reproductive capacity, assessing their biocontrol by using entomopathogenic fungi is crucial; to determine their potential, fungi were tested in planta and in field conditions. Significant decrease of Myzus persicae (Sulzer) population was observed in planta after applying Beauveria bassiana (strain 7R), Trichoderma gamsii (strain Z) or Metarhizium brunneum (strain Meta Br1) at 1 × 10 or 1 × 10 conidia/mL on pepper plants. Significant differences of aphids' populations were detected between fungus concentration and control (F = 68.

In Vitro Antitumor Activity of Endophytic and Rhizosphere Gram-Positive Bacteria from (S. Watson) Greene against L5178Y-R Lymphoma Cells.

Plant-associated microorganisms represent a potential source of new antitumor compounds. The aim of the present study was to isolate endophytic and rhizosphere Gram-positive bacteria from and produce extracts with antitumor activity. Methanol and ethyl acetate extracts were obtained from 28 d bacterial fermentation, after which murine L5178Y-R lymphoma cells growth inhibition was evaluated at concentrations ranging from 15.

Antimicrobial Effect of Calcium Hydroxide Combined with Electrolyzed Superoxidized Solution at Neutral pH on Growth.

Objective: To evaluate the effect of the combination of calcium hydroxide (Ca(OH)) and a novel electrolyzed superoxidized solution at neutral pH, known as OxOral® on growth in root canals.

Methods: Sixty human teeth were used, from which root canals were infected and randomly divided into the following treatment groups: saline solution, saline solution plus Ca(OH), OxOral®, and OxOral® plus Ca(OH).

Results: A permanent reduction in bacterial growth was observed at days 1, 6, 12, and 18 after OxOral® plus Ca(OH) treatment from 4.

In Vitro Tumor Cell Growth Inhibition Induced by (DC.) S. Arias and Terrazas Endophytic Fungi Extracts.

Endophytic fungi have become potential sources of antitumor agents, particularly against antineoplastic-resistant cancer cells, with marginal or nil adverse effects for the oncological patient. Endophytic fungi were isolated from stems of the cactus, commonly found in Mexico. Methanol extracts were then obtained from fungus liquid cultures and their effects on tumor cell growth against murine lymphoma (L5178Y-R), human colorectal adenocarcinoma (HT-29), and human breast cancer (MCF-7) cells were evaluated at concentrations ranging from 31 µg/mL to 250 µg/mL via the colorimetric 3- [4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazoliumbromide reduction assay, using monkey kidney epithelial (MA-104) and human peripheral mononuclear (PBMC) cells as controls.

Mycelial compatibility, anastomosis, and nucleus numbers of eight Mexican strains isolated from .

Background: Among entomopathogenic fungi, has been recognized as potential biocontrol agent against the Asian citrus psyllid (Hemiptera: Liviidae). Nevertheless, this fungus is poorly characterized. Previous molecular studies have shown high sequence similarities among strains, but significant differences in virulence.